MG1655
W3110
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Gene Report : araD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Carcinogenesis. 1993;14(2):303-5.
A method for selection of forward mutations in supF gene carried by shuttle-vector plasmids.
Ariza RR, Roldan-Arjona T, Hera C, Pueyo C. ( 8435871 )
     Deletion OCR29 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  araD
Related strains
     Strains Search Search strains by gene name:  araD   Search strains by all related name:  araD b0061 ECK0062 JW0060

General information  (Go to Linear View:)
 Gene Name araD  
 Alternative name b0061,ECK0062,JW0060  
 Location, Length 65,855 - 66,550 (  -  ) ;   1.42 min ; 696 (bp) ,   231 (aa) Go to Linear View
 Product L-ribulose-5-phosphate 4-epimerase  
 Operon Name araBAD  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function enzyme; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0005975; carbohydrate metabolic process ( araD )
GO:0008270; zinc ion binding ( araD )
GO:0008742; L-ribulose-phosphate 4-epimerase activity ( araD )
GO:0016853; isomerase activity ( araD )
GO:0019568; arabinose catabolic process ( araD )
GO:0019572; L-arabinose catabolic process ( araD )
GO:0046872; metal ion binding ( araD )
 PID 1786247  
 EC number
  (KEGG Pathway)
 
5.1.3.4  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) ARAD_ECOLI ( P08203 )
    -  Protein name L-ribulose-5-phosphate 4-epimerase  
    -  Synonyms EC 5.1.3.4, Phosphoribulose isomerase  
    -  Gene name Name=araD; OrderedLocusNames=b0061;  

 Linear View (Whole Mode)
View Location
   55.0  –  80.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP araD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of L-Ribulose-5-Phosphate 4-Epimerase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST L-ribulose-5-phosphate 4-epimerase  
         PSI-BLAST L-ribulose-5-phosphate 4-epimerase
    nr     (database updated : 2007.02.20 )
         BLAST L-ribulose-5-phosphate 4-epimerase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0235G 
    EcoCyc araD 

MMBR References
Gene. 1986;47(2-3):231-44.
The organization of the araBAD operon of Escherichia coli.
Lee N, Gielow W, Martin R, Hamilton E, Fowler A. ( 3549454 )
Nucleic Acids Res. 1990;18(22):6722
Nucleotide sequence of the araD gene of Escherichia coli K12 encoding the L-ribulose 5-phosphate 4-epimerase.
Mineno J, Fukui H, Ishino Y, Kato I, Shinagawa H. ( 2251150 )

Sequences
Amino acid
FASTA format
0001 MLEDLKRQVL EANLALPKHN LVTLTWGNVS AVDRERGVFV IKPSGVDYSV MTADDMVVVS IETGEVVEGT 
0071 KKPSSDTPTH RLLYQAFPSI GGIVHTHSRH ATIWAQAGQS IPATGTTHAD YFYGTIPCTR KMTDAEINGE 
0141 YEWETGNVIV ETFEKQGIDA AQMPGVLVHS HGPFAWGKNA EDAVHNAIVL EEVAYMGIFC RQLAPQLPDM 
0211 QQTLLDKHYL RKHGAKAYYG Q

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             cattcaacgc ctgatgcgac gctggcgcgt 
-070 cttatcaggc ctacacgctg cgattttgta ggccggataa gcaaagcgca tccggcacga aggagtcaac 
0001 atgttagaag atctcaaacg ccaggtatta gaagccaacc tggcgctgcc aaaacacaac ctggtcacgc 
0071 tcacatgggg caacgtcagc gccgttgatc gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga 
0141 ttacagcgtc atgaccgctg acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg 
0211 aaaaagccct cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg 
0281 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa ccggcaccac 
0351 ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg acgcagaaat caacggcgaa 
0421 tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg aaaaacaggg tatcgatgca gcgcaaatgc 
0491 ccggcgttct ggtccattcc cacggcccgt ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc 
0561 catcgtgctg gaagaggtcg cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg 
0631 cagcaaacgc tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac 
0701 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg gattttcagc 
0771 gtggcgcagg caggttttat cttaac