|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
J Bacteriol. 1990;172(6):3146-51.
Escherichia coli rna gene encoding RNase I: cloning, overexpression, subcellular distribution of the enzyme, and use of an rna deletion to identify additional RNases.
Zhu LQ, Gangopadhyay T, Padmanabha KP, Deutscher MP.
(
2188952
)
|
|
|
Deletion
|
OCR45,46-1
(D)
,
OCR45,46-2
(D)
,
OCR45,46-3
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
rna
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
rna
Search strains by all related name:
rna b0611 ECK0604 JW0603 rnsA
|
General information (Go to Linear View: )
|
|
Gene Name
|
rna
|
|
Alternative name
|
b0611,ECK0604,JW0603,rnsA
|
|
Location, Length
|
643,420 - 644,226
(
-
)
;
13.87
min
;
807
(bp) ,
268
(aa)
|
|
Product
|
ribonuclease I
|
|
Operon Name
|
rna
|
|
Note
|
RNase I, cleaves phosphodiester bond between any two nucleotides; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006401 - RNA catabolic process
|
|
Function
|
enzyme; Degradation of RNA
|
|
Gene Ontology
|
GO:0003723
;
RNA binding ( rna )
GO:0004518
;
nuclease activity ( rna )
GO:0004519
;
endonuclease activity ( rna )
GO:0004521
;
endoribonuclease activity ( rna )
GO:0005737
;
cytoplasm ( rna )
GO:0008847
;
Enterobacter ribonuclease activity ( rna )
GO:0016787
;
hydrolase activity ( rna )
GO:0042597
;
periplasmic space ( rna )
|
|
PID
|
1786828
|
EC number
(KEGG Pathway)
|
3.1.27.6
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
rna
(
EG10856
)
|
|
Number of removed
|
23 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
RNI_ECOLI
(
P21338
;
P77101
)
|
|
- Protein name
|
Ribonuclease I precursor
|
|
- Synonyms
|
EC 3.1.27.6, Enterobacter ribonuclease, RNase I
|
|
- Gene name
|
Name=rna; Synonyms=rnsA; OrderedLocusNames=b0611;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
rna
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Rnase Le
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Ribonuclease I precursor
|
|
PSI-BLAST
|
Ribonuclease I precursor
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
Enterobacter ribonuclease [Escherichia coli B]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
RNASE_T2_1
RNASE_T2_2
|
|
MMBR References
|
Gene. 1990;95(1):1-7.
Cloning and sequencing the gene encoding Escherichia coli ribonuclease I: exact physical mapping using the genome library.
Meador J 3rd, Kennell D.
(
2253883
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKAFWRNAAL LAVSLLPFSS ANALALQAKQ YGDFDRYVLA LSWQTGFCQS QHDRNRNERD ECRLQTETTN
0071 KADFLTVHGL WPGLPKSVAA RGVDERRWMR FGCATRPIPN LPEARASRMC SSPETGLSLE TAAKLSEVMP
0141 GAGGRSCLER YEYAKHGACF GFDPDAYFGT MVRLNQEIKE SEAGKFLADN YGKTVSRRDF DAAFAKSWGK
0211 ENVKAVKLTC QGNPAYLTEI QISIKADAIN APLSANSFLP QPHPGNCGKT FVIDKAGY
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 cgctgaataa atcatcacgc ccgccaggta
-070 agcccacctg gcgggctttt ttatgattta atagatagta ttcagtgtat gtatgagttc cacacccatt
0001 atgaaagcat tctggcgtaa cgccgcgttg ctcgcggttt ctctgcttcc cttctcttct gccaacgcct
0071 tagcgttgca ggcaaaacag tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc aaaccggatt
0141 ttgccagagt caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac
0211 aaagctgatt ttctgaccgt acatggtctg tggccaggat tgcctaaatc ggttgctgcc cgtggtgttg
0281 atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat ctaccagaag cgcgcgccag
0351 ccgaatgtgt tcatcgccgg aaaccggatt atcactggaa acggccgcta aactaagtga agtcatgcca
0421 ggagctggcg gacgttcctg cctggaacgc tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc
0491 cggacgcata cttcggtacg atggtacgcc tgaatcaaga aattaaagag agcgaagccg gaaaattcct
0561 tgcggataat tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct ttgccaaaag ctggggaaaa
0631 gagaacgtga aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt cagatctcga
0701 tcaaagctga cgccatcaac gctccgcttt ctgcaaactc attcttgcca caacctcacc caggtaactg
0771 tggcaaaacc tttgtgattg ataaagcggg ttattaatca tctgatttac aaaacacgct aattcgtctt
0841 ccataaacca aggcgattaa gatttataca taatgcgctg cattgaggaa ttccctgaat gtgacgc
|
