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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCR43
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
purK
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Related strains
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Strains Search
|
Search strains by gene name:
purK
Search strains by all related name:
purK b0522 ECK0515 JW0511 purE2
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General information (Go to Linear View: )
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Gene Name
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purK
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Alternative name
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b0522,ECK0515,JW0511,purE2
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Location, Length
|
550,750 - 551,817
(
-
)
;
11.87
min
;
1068
(bp) ,
355
(aa)
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Product
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N5-carboxyaminoimidazole ribonucleotide synthase
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Operon Name
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purEK
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Note
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phosphoribosylaminoimidazole carboxylase = AIR carboxylase, CO(2)-fixing subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006164 - purine nucleotide biosynthetic process
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Function
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enzyme; Purine ribonucleotide biosynthesis
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Gene Ontology
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GO:0000166
;
nucleotide binding ( purK )
GO:0003824
;
catalytic activity ( purK )
GO:0004638
;
phosphoribosylaminoimidazole carboxylase activity ( purK )
GO:0005524
;
ATP binding ( purK )
GO:0006164
;
purine nucleotide biosynthetic process ( purK )
GO:0006189
;
'de novo' IMP biosynthetic process ( purK )
GO:0009320
;
phosphoribosylaminoimidazole carboxylase complex ( purK )
GO:0016829
;
lyase activity ( purK )
GO:0016831
;
carboxy-lyase activity ( purK )
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PID
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1786733
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EC number
(KEGG Pathway)
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4.1.1.21
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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purK
(
EG10796
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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PURK_ECOLI
(
P09029
)
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- Protein name
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Phosphoribosylaminoimidazole carboxylase ATPase subunit
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- Synonyms
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EC 4.1.1.21, AIR carboxylase, AIRC
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- Gene name
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Name=purK; OrderedLocusNames=b0522;
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MMBR References
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Gene. 1986;44(1):55-62.
Molecular cloning and characterization of the purE operon of Escherichia coli.
Kamholz J, Keyhani J, Gots JS.
(
3021590
)
|
J Bacteriol. 1989;171(1):205-12.
Nucleotide sequence analysis of the purEK operon encoding 5'-phosphoribosyl-5-aminoimidazole carboxylase of Escherichia coli K-12.
Tiedeman AA, Keyhani J, Kamholz J, Daum HA 3rd, Gots JS, Smith JM.
(
2464576
)
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| Sequences |
Amino acid
FASTA format
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0001 MKQVCVLGNG QLGRMLRQAG EPLGIAVWPV GLDAEPAAVP FQQSVITAEI ERWPETALTR ELARHPAFVN
0071 RDVFPIIADR LTQKQLFDKL HLPTAPWQLL AERSEWPAVF DRLGELAIVK RRTGGYDGRG QWRLRANETE
0141 QLPAECYGEC IVEQGINFSG EVSLVGARGF DGSTVFYPLT HNLHQDGILR TSVAFPQANA QQQAQAEEML
0211 SAIMQELGYV GVMAMECFVT PQGLLINELA PRVHNSGHWT QNGASISQFE LHLRAITDLP LPQPVVNNPS
0281 VMINLIGSDV NYDWLKLPLV HLHWYDKEVR PGRKVGHLNL TDSDTSRLTA TLEALIPLLP PEYASGVIWA
0351 QSKFG
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttcttgcgac tcatgataaa gaactgcacc
-070 agcgtctgaa tgactggcgc aaagcccaga ccgacgaagt gctggaaaac ccggacccgc gaggtgcggc
0001 atgaaacagg tttgcgtcct cggtaacggg cagttaggcc gtatgctgcg tcaggcaggc gaaccgttag
0071 gcattgctgt ctggccagtc gggctggacg ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac
0141 cgctgagata gaacgctggc cggaaaccgc attaacccgc gagctggcgc gccatccggc ctttgtgaac
0211 cgcgatgtgt tcccgattat tgctgaccgt ctgactcaga agcagctttt cgataagctc cacctgccga
0281 ctgcaccgtg gcagttactt gccgaacgca gcgagtggcc tgcggtgttt gatcgtttag gtgagctggc
0351 gattgttaag cgtcgcactg gtggttatga cggtcgcggt caatggcgtt tacgcgcaaa tgaaaccgaa
0421 cagttaccgg cagagtgtta cggcgaatgt attgtcgagc agggcattaa cttctctggt gaagtgtcgc
0491 tggttggcgc gcgcggcttt gatggcagca ccgtgtttta tccgctgacg cataacctgc atcaggacgg
0561 tattttgcgc accagcgtcg cttttccgca ggccaacgca cagcagcagg cgcaagccga agagatgctg
0631 tcggcgatta tgcaggagct gggctatgtg ggcgtgatgg cgatggagtg ttttgtcacc ccgcaaggtc
0701 tgttgatcaa cgaactggca ccgcgtgtgc ataacagcgg tcactggaca caaaacggtg ccagcatcag
0771 ccagtttgag ctgcatctgc gggcgattac cgatctgccg ttaccgcaac cagtggtgaa taatccgtcg
0841 gtgatgatca atctgattgg tagcgatgtg aattatgact ggctgaaact gccgctggtg catctgcact
0911 ggtacgacaa agaagtccgt ccggggcgta aagtggggca tctgaatttg accgacagcg acacatcgcg
0981 tctgactgcg acgctggaag ccttaatccc gctgctgccg ccggaatatg ccagcggcgt gatttgggcg
1051 cagagtaagt tcggttaact ggtgctctat tcttgccgga tgcggcgtaa acgccttatc cggcctaccg
1121 atccggtacc cattgtaggc ctgataagat gcgtcaagca tcgcatca
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