|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
essential
|
|
References (PMID)
|
Mol Gen Genet. 1997;255(4):376-81.
Effects of starvation for heme on the synthesis of porphyrins in Escherichia coli.
Nakayashiki T, Inokuchi H.
(
9267433
)
|
|
|
Deletion
|
OCR42
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
hemH
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
hemH
Search strains by all related name:
hemH b0475 ECK0469 JW0464 popA visA
|
General information (Go to Linear View: )
|
|
Gene Name
|
hemH
|
|
Alternative name
|
b0475,ECK0469,JW0464,popA,visA
|
|
Location, Length
|
497,279 - 498,241
(
+
)
;
10.72
min
;
963
(bp) ,
320
(aa)
|
|
Product
|
ferrochelatase
|
|
Operon Name
|
hemH
|
|
Note
|
ferrochelatase: final enzyme of heme biosynthesis
|
|
Function
|
enzyme; Biosynthesis of cofactors, carriers: Heme, porphyrin
|
|
Gene Ontology
|
GO:0004325
;
ferrochelatase activity ( hemH )
GO:0005506
;
iron ion binding ( hemH )
GO:0005737
;
cytoplasm ( hemH )
GO:0006779
;
porphyrin biosynthetic process ( hemH )
GO:0006783
;
heme biosynthetic process ( hemH )
GO:0016829
;
lyase activity ( hemH )
GO:0046872
;
metal ion binding ( hemH )
|
|
PID
|
1786681
|
EC number
(KEGG Pathway)
|
4.99.1.1
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
hemH
(
EG10431
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
HEMZ_ECOLI
(
P23871
;
P78232
)
|
|
- Protein name
|
Ferrochelatase
|
|
- Synonyms
|
EC 4.99.1.1, Protoheme ferro-lyase, Heme synthetase
|
|
- Gene name
|
Name=hemH; Synonyms=popA, visA; OrderedLocusNames=b0475;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
hemH
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Human Ferrochelatase
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Ferrochelatase
|
|
PSI-BLAST
|
Ferrochelatase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
ferrochelatase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
FERROCHELATASE
|
|
MMBR References
|
J Bacteriol. 1993;175:2154-2156
The Escherichia coli visA gene encodes ferrochelatase, the final enzyme of the heme biosynthetic pathway.
Frustaci, J. M., and M. R. O'Brian.
|
J Mol Biol. 1991;219(3):393-8.
Isolation and characterization of visible light-sensitive mutants of Escherichia coli K12.
Miyamoto K, Nakahigashi K, Nishimura K, Inokuchi H.
(
2051480
)
|
J Bacteriol. 1992;174:7352-7359
Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone.
Nakahigashi, K., K. Miyamoto, K. Nishimura, and H. Inokuchi.
|
Proc Natl Acad Sci U S A. 1991;88:10520-10524
Photosensitivity of a protoporphyrin-accumulating, light-sensitive mutant (visA) of Escherichia coli K-12.
Nakahigashi, K., K. Nishimura, K. Miyamoto, and H. Inokuchi.
|
| Sequences |
Amino acid
FASTA format
|
0001 MRQTKTGILL ANLGTPDAPT PEAVKRYLKQ FLSDRRVVDT SRLLWWPLLR GVILPLRSPR VAKLYASVWM
0071 EGGSPLMVYS RQQQQALAQR LPEMPVALGM SYGSPSLESA VDELLAEHVD HIVVLPLYPQ FSCSTVGAVW
0141 DELARILARK RSIPGISFIR DYADNHDYIN ALANSVRASF AKHGEPDLLL LSYHGIPQRY ADEGDDYPQR
0211 CRTTTRELAS ALGMAPEKVM MTFQSRFGRE PWLMPYTDET LKMLGEKGVG HIQVMCPGFA ADCLETLEEI
0281 AEQNREVFLG AGGKKYEYIP ALNATPEHIE MMANLVAAYR
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCGCTATTCC TTTTTCTGAT TTGACCTCTC
-070 ACAGCAATTA GTTCTTCTTC CTCACTTTTC CGCTACAATT ATCAACAAGT TGAATCGATA AGAGGCGGTA
0001 atgcgtcaga ctaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg
0071 taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt tatggtggcc
0141 attgctgcgc ggcgtgattt tgccgctgcg ctcgccgcgt gtggcgaagc tgtatgcctc tgtctggatg
0211 gaaggtggct cgccgctgat ggtttacagc cgccagcaac agcaggcgct ggcacaacgt ttaccggaga
0281 tgcccgtagc gctgggaatg agctacggct cgccatcact ggaaagcgcc gtagatgaac tcctggcaga
0351 gcatgtagat catattgtgg tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg
0421 gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttatacgt gattacgccg
0491 ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt gccaaacatg gcgaaccgga
0561 tctgctactg ctctcttatc atggcattcc ccagcgttat gcagatgaag gcgatgatta cccgcaacgt
0631 tgccgcacaa cgactcgtga actggcttcc gcattgggga tggcaccgga aaaagtgatg atgacctttc
0701 agtcgcgctt tggtcgggaa ccctggctga tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa
0771 aggcgtaggt catattcagg tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt
0841 gccgagcaaa accgtgaggt cttcctcggt gccggcggga aaaaatatga atatattccg gcgcttaatg
0911 ccacgccgga acatatcgaa atgatggcta atcttgttgc cgcgtatcgc taaAGCTGAG CGGTAAAGAA
0981 CTGAGCGCCG TCGCGAAGAG CCTCGTCGGC GGTTTTCATC ATCCGTGAAT AATGCAAAAA GGCGTGCAGC
1051 GTGCCTGGGT AGA
|
