|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCR40
(D)
,
OCR40-2
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
clpX
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
clpX
Search strains by all related name:
clpX b0438 ECK0432 JW0428 lopC
|
General information (Go to Linear View: )
|
|
Gene Name
|
clpX
|
|
Alternative name
|
b0438,ECK0432,JW0428,lopC
|
|
Location, Length
|
456,650 - 457,924
(
+
)
;
9.84
min
;
1275
(bp) ,
424
(aa)
|
|
Product
|
ATPase and specificity subunit of ClpX-ClpP ATP-dependent serine protease
|
|
Operon Name
|
clpPX-lon
|
|
Note
|
ATP-dependent specificity component of clpP serine protease, chaperone; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006457 - protein folding
|
|
Function
|
enzyme; Degradation of proteins, peptides, glyco
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( clpX )
GO:0005515
;
protein binding ( clpX )
GO:0005524
;
ATP binding ( clpX )
GO:0006457
;
protein folding ( clpX )
GO:0006950
;
response to stress ( clpX )
GO:0008270
;
zinc ion binding ( clpX )
GO:0015031
;
protein transport ( clpX )
GO:0016887
;
ATPase activity ( clpX )
GO:0017111
;
nucleoside-triphosphatase activity ( clpX )
GO:0019538
;
protein metabolic process ( clpX )
GO:0046872
;
metal ion binding ( clpX )
GO:0046983
;
protein dimerization activity ( clpX )
GO:0051082
;
unfolded protein binding ( clpX )
|
|
PID
|
1786642
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
clpX
(
EG10159
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
CLPX_ECOLI
(
P33138
)
|
|
- Protein name
|
ATP-dependent Clp protease ATP-binding subunit clpX.
|
|
- Synonyms
|
|
|
- Gene name
|
OrderedLocusNames=b0438, c0554, z0543, ECs0492, SF0383, S0389;
|
|
MMBR References
|
Proc Natl Acad Sci U S A. 1996;93:10291-10296
Regulation of SOS mutagenesis by proteolysis.
Frank, E. G., D. G. Ennis, M. Gonzalez, A. S. Levine, and R. Woodgate.
|
J Biol Chem. 1993;268:22618-22626
ClpX, an alternative subunit for the ATP-dependent Clp protease of Escherichia coli. Sequence and in vivo activities.
Gottesman, S., W. P. Clark, V. de Crecy-Lagard, and M. R. Maurizi.
|
Genes Dev. 1995;9:2399-2408
Disassembly of the Mu transposase tetramer by the ClpX chaperone.
Levchenko, I., L. Luo, and T. A. Baker.
|
J Biol Chem. 1993;268:22609-22617
Isolation and characterization of ClpX, a new ATP-dependent specificity component of the Clp protease of Escherichia coli.
Wojkowiak, D., C. Georgopoulos, and M. Zylicz.
|
Biochem Biophys Res Commun. 1994;203(2):798-804.
clpX encoding an alternative ATP-binding subunit of protease Ti (Clp) can be expressed independently from clpP in Escherichia coli.
Yoo SJ, Seol JH, Kang MS, Ha DB, Chung CH.
(
8093059
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTDKRKDGSG KLLYCSFCGK SQHEVRKLIA GPSVYICDEC VDLCNDIIRE EIKEVAPHRE RSALPTPHEI
0071 RNHLDDYVIG QEQAKKVLAV AVYNHYKRLR NGDTSNGVEL GKSNILLIGP TGSGKTLLAE TLARLLDVPF
0141 TMADATTLTE AGYVGEDVEN IIQKLLQKCD YDVQKAQRGI VYIDEIDKIS RKSDNPSITR DVSGEGVQQA
0211 LLKLIEGTVA AVPPQGGRKH PQQEFLQVDT SKILFICGGA FAGLDKVISH RVETGSGIGF GATVKAKSDK
0281 ASEGELLAQV EPEDLIKFGL IPEFIGRLPV VATLNELSEE ALIQILKEPK NALTKQYQAL FNLEGVDLEF
0351 RDEALDAIAK KAMARKTGAR GLRSIVEAAL LDTMYDLPSM EDVEKVVIDE SVIDGQSKPL LIYGKPEAQQ
0421 ASGE
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TACTTATCCA GGGCGGCACA ACGCTGTAAG
-070 CGGCTTGCGC CTGAGAATGG CATTTGCGTC GTCGTGTGCG GCACAAAGAA CAAAGAAGAG GTTTTGACCC
0001 atgacagata aacgcaaaga tggctcaggc aaattgctgt attgctcttt ttgcggcaaa agccagcatg
0071 aagtgcgcaa gctgattgcc ggtccatccg tgtatatctg cgacgaatgt gttgatttat gtaacgacat
0141 cattcgcgaa gagattaaag aagttgcacc gcatcgtgaa cgcagtgcgc taccgacgcc gcatgaaatt
0211 cgcaaccacc tggacgatta cgttatcggc caggaacagg cgaaaaaagt gctggcggtc gcggtataca
0281 accattacaa acgtctgcgc aacggcgata ccagcaatgg cgtcgagttg ggcaaaagta acattctgct
0351 gatcggtccg accggttccg gtaaaacgct gctggctgaa acgctggcgc gcctgctgga tgttccgttc
0421 accatggccg acgcgactac actgaccgaa gccggttatg tgggtgaaga cgttgaaaac atcattcaga
0491 agctgttgca gaaatgcgac tacgatgtcc agaaagcaca gcgtggtatt gtctacatcg atgaaatcga
0561 caagatttct cgtaagtcag acaacccgtc cattacccga gacgtttccg gtgaaggcgt acagcaggca
0631 ctgttgaaac tgatcgaagg tacggtagct gctgttccac cgcaaggtgg gcgtaaacat ccgcagcagg
0701 aattcttgca ggttgatacc tctaagatcc tgtttatttg tggcggtgcg tttgccggtc tggataaagt
0771 gatttcccac cgtgtagaaa ccggctccgg cattggtttt ggcgcgacgg taaaagcgaa gtccgacaaa
0841 gcaagcgaag gcgagctgct ggcgcaggtt gaaccggaag atctgatcaa gtttggtctt atccctgagt
0911 ttattggtcg tctgccggtt gtcgcaacgt tgaatgaact gagcgaagaa gctctgattc agatcctcaa
0981 agagccgaaa aacgccctga ccaagcagta tcaggcgctg tttaatctgg aaggcgtgga tctggaattc
1051 cgtgacgagg cgctggatgc tatcgctaag aaagcgatgg cgcgtaaaac cggtgcccgt ggcctgcgtt
1121 ccatcgtaga agccgcactg ctcgatacca tgtacgatct gccgtccatg gaagacgtcg aaaaagtggt
1191 tatcgacgag tcggtaattg atggtcaaag caaaccgttg ctgatttatg gcaagccgga agcgcaacag
1261 gcatctggtg aataaTTAAC CATTCCCATA CAATTAGTTA ACCAAAAAGG GGGGATTTTA TCTCCCCTTT
1331 AATTTTTCCT CTATTCTCGG CGTTGAATGT GGGGGAAACA TCCCC
|
