|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
essential
|
|
References (PMID)
|
|
|
Deletion
|
OCL60,61-1
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
ligA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
ligA
Search strains by all related name:
ligA b2411 dnaL ECK2406 JW2403 lig ligA lop pdeC
|
General information (Go to Linear View: )
|
|
Gene Name
|
ligA
|
|
Alternative name
|
b2411,dnaL,ECK2406,JW2403,lig,ligA,lop,pdeC
|
|
Location, Length
|
2,526,183 - 2,528,198
(
-
)
;
54.45
min
;
2016
(bp) ,
671
(aa)
|
|
Product
|
DNA ligase, NAD(+)-dependent
|
|
Operon Name
|
ligA-ypeB
|
|
Note
|
DNA ligase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006281 - DNA repair
|
|
Function
|
enzyme; DNA - replication, repair,restriction/modification
|
|
Gene Ontology
|
GO:0003677
;
DNA binding ( ligA )
GO:0003911
;
DNA ligase (NAD+) activity ( ligA )
GO:0005622
;
intracellular ( ligA )
GO:0006259
;
DNA metabolic process ( ligA )
GO:0006260
;
DNA replication ( ligA )
GO:0006281
;
DNA repair ( ligA )
GO:0006974
;
response to DNA damage stimulus ( ligA )
GO:0016874
;
ligase activity ( ligA )
GO:0043565
;
sequence-specific DNA binding ( ligA )
|
|
PID
|
1788750
|
EC number
(KEGG Pathway)
|
6.5.1.2
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
ligA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Nad+-Dependent Dna Ligase From T. Filiformis
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
DNA ligase
|
|
PSI-BLAST
|
DNA ligase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
NAD-dependent DNA ligase LigA [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
|
|
MMBR References
|
Proc Natl Acad Sci U S A. 1970;67:1580-1587
DNA ligase mutant of Escherichia coli.
Gellert, M., and M. L. Bullock.
|
Mol Gen Genet. 1986;204(1):1-7.
Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.
Ishino Y, Shinagawa H, Makino K, Tsunasawa S, Sakiyama F, Nakata A.
(
3018436
)
|
Biochem Biophys Res Commun. 1996;226(2):498-505.
Prokaryotic DNA ligases unwind superhelical DNA.
Ivanchenko M, van Holde K, Zlatanova J.
(
8806663
)
|
Proc Natl Acad Sci U S A. 1977;74:3485-3489
Large-scale automated isolation of Escherichia coli mutants with thermosensitive DNA replication.
Sevastopoulis, C. G., C. T. Wehr, and D. A. Glaser.
|
| Sequences |
Amino acid
FASTA format
|
0001 MESIEQQLTE LRTTLRHHEY LYHVMDAPEI PDAEYDRLMR ELRELETKHP ELITPDSPTQ RVGAAPLAAF
0071 SQIRHEVPML SLDNVFDEES FLAFNKRVQD RLKNNEKVTW CCELKLDGLA VSILYENGVL VSAATRGDGT
0141 TGEDITSNVR TIRAIPLKLH GENIPARLEV RGEVFLPQAG FEKINEDARR TGGKVFANPR NAAAGSLRQL
0211 DPRITAKRPL TFFCYGVGVL EGGELPDTHL GRLLQFKKWG LPVSDRVTLC ESAEEVLAFY HKVEEDRPTL
0281 GFDIDGVVIK VNSLAQQEQL GFVARAPRWA VAFKFPAQEQ MTFVRDVEFQ VGRTGAITPV ARLEPVHVAG
0351 VLVSNATLHN ADEIERLGLR IGDKVVIRRA GDVIPQVVNV VLSERPEDTR EVVFPTHCPV CGSDVERVEG
0421 EAVARCTGGL ICGAQRKESL KHFVSRRAMD VDGMGDKIID QLVEKEYVHT PADLFKLTAG KLTGLERMGP
0491 KSAQNVVNAL EKAKETTFAR FLYALGIREV GEATAAGLAA YFGTLEALEA ASIEELQKVP DVGIVVASHV
0561 HNFFAEESNR NVISELLAEG VHWPAPIVIN AEEIDSPFAG KTVVLTGSLS QMSRDDAKAR LVELGAKVAG
0631 SVSKKTDLVI AGEAAGSKLA KAQELGIEVI DEAEMLRLLG S
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 atccgcgaag tcaaagacgc caacgcctga
-070 tacacttaag gcaaattaac tcctcttcga acccccgctt gtcgggggtt tttagcattg atggtgcgat
0001 atggaatcaa tcgaacaaca actgacagaa ctgcgaacga cgcttcgcca tcatgaatat ctttatcatg
0071 tgatggatgc gccggaaatt cccgacgctg aatacgacag gctgatgcgc gaactgcgcg agctggaaac
0141 caaacatcca gaactgatta cgcctgattc gcctactcaa cgtgtaggcg ctgcgccgct ggcggctttc
0211 agccagatac gccatgaagt accaatgctg tcactggata acgtttttga tgaagaaagc tttcttgctt
0281 tcaacaaacg tgtgcaggac cgtctgaaaa acaacgagaa agtcacctgg tgctgtgagc tgaagctgga
0351 tggtcttgcc gtcagtattc tgtatgaaaa tggcgtttta gtcagtgccg cgacccgtgg cgatggcacc
0421 accggggaag atatcacgtc taatgtgcgt actattcgcg ccattccgct gaagctgcac ggagagaata
0491 tcccggcgcg tctggaagtg cgtggtgaag tgttcctgcc gcaggcgggg ttcgaaaaga ttaacgaaga
0561 tgcgcgacgc acgggcggga aagtgtttgc taacccacgt aatgcggcag ctggttcact gcgtcagctt
0631 gatccgcgta ttacagcgaa gcgaccgctc acttttttct gctatggcgt tggtgttctg gaaggtggcg
0701 agctgccgga tactcatctt ggccgtttac tgcaatttaa aaagtggggg ttgccggtca gcgatcgggt
0771 aacgctttgt gaatcggcgg aagaagtgct ggcgttctat cacaaagtgg aagaagaccg cccgacgctg
0841 ggctttgata tcgacggcgt ggtgattaag gtcaactcac tggcacagca ggagcagctt ggctttgtcg
0911 cgcgtgcccc gcgctgggcg gtagcgttta aattcccggc gcaggagcag atgacctttg tgcgtgacgt
0981 cgagtttcag gttgggcgta ctggcgcgat tacgcctgtt gcgcgtctgg aacctgtcca tgttgcaggc
1051 gtgctggtga gtaacgcaac cttacacaat gcggatgaaa tcgaacgtct tggtttacgc attggcgata
1121 aagtggtgat tcgccgcgct ggcgacgtga tcccgcaggt ggttaacgtc gtgctttctg aacgcccgga
1191 agatacccgt gaggttgtat tcccgacgca ttgtccggta tgtggttctg acgttgagcg tgtggaaggt
1261 gaagcggttg cccgctgtac cggtggcctg atttgcggtg cgcagcgtaa agagtcgctg aaacactttg
1331 tttcccgccg tgcgatggat gttgacggaa tgggcgacaa aatcatcgat cagctggttg aaaaagaata
1401 tgtccatact ccggcagatc tgttcaaact caccgcaggc aaactgaccg gactggagcg tatggggcca
1471 aaatcggcac aaaacgtggt taacgcgctg gaaaaagcga aagaaaccac ctttgctcgc ttcctctatg
1541 cacttggcat ccgtgaagtc ggcgaggcca ccgcagcagg tctggcggca tatttcggca cgctggaagc
1611 gctggaagcc gcttcgattg aagagctgca aaaggtgcct gatgttggca ttgtcgttgc atcccacgtt
1681 cacaacttct ttgccgaaga aagcaaccgc aatgtcatca gcgagctgtt ggcggaaggt gttcactggc
1751 ctgcgccgat cgttatcaac gcggaagaga ttgacagccc gtttgctggt aaaaccgtgg tgcttacggg
1821 cagcttaagc cagatgtcgc gtgatgacgc taaagctcga ctggtcgaac tgggcgcgaa agtcgcgggc
1891 agcgtgtcga agaaaaccga tctggtgata gcgggtgaag ctgcaggatc taaactggcg aaggcgcagg
1961 aactgggcat tgaagtcatc gacgaagcgg aaatgctgcg tttgctgggt agctgagatg gaaaaagagc
2031 agctgattga aatagccaat acgataatgc cgtttggcaa atacaaaggg cgtcgcttaa tcgacctgcc
2101 ggaggagtat ctgctg
|
