MG1655
W3110
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Gene Report : hcaE
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
J Bacteriol. 1998;180(11):2915-23.
Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12.
Diaz E, Ferrandez A, Garcia JL. ( 9603882 )
     Deletion OCL55 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  hcaE
Related strains
     Strains Search Search strains by gene name:  hcaE   Search strains by all related name:  hcaE b2538 digA ECK2535 hcaA hcaA1 JW2522 phdC1 yfhU

General information  (Go to Linear View:)
 Gene Name hcaE  
 Alternative name b2538,digA,ECK2535,hcaA,hcaA1,JW2522,phdC1,yfhU  
 Location, Length 2,667,054 - 2,668,415 (  +  ) ;   57.48 min ; 1362 (bp) ,   453 (aa) Go to Linear View
 Product 3-phenylpropionate dioxygenase, large (alpha) subunit  
 Operon Name hcaEFCBD  
 Note GO_process: GO:0016052 - carbohydrate catabolic process  
 Function enzyme; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0005506 ; iron ion binding ( hcaE )
GO:0005515 ; protein binding ( hcaE )
GO:0006118 ; electron transport ( hcaE )
GO:0006725 ; aromatic compound metabolic process ( hcaE )
GO:0008695 ; 3-phenylpropionate dioxygenase activity ( hcaE )
GO:0016491 ; oxidoreductase activity ( hcaE )
GO:0016702 ; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen ( hcaE )
GO:0019439 ; aromatic compound catabolic process ( hcaE )
GO:0046872 ; metal ion binding ( hcaE )
GO:0051536 ; iron-sulfur cluster binding ( hcaE )
GO:0051537 ; 2 iron, 2 sulfur cluster binding ( hcaE )
 PID 1788888  
 EC number
  (KEGG Pathway)   		1.14.12.19  

 Linear View (Whole Mode)
View Location
   2655.0  –  2680.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP hcaE (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Biphenyl Dioxygenase (Bpha1a2) Derived From Rhodococcus Sp. Strain Rha1  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 3-phenylpropionate dioxygenase subunit alpha (Digoxigenin subunit alpha)  
         PSI-BLAST 3-phenylpropionate dioxygenase subunit alpha (Digoxigenin subunit alpha)
    nr     (database updated : 2007.02.20 )
         BLAST large terminal subunit of phenylpropionate dioxygenase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    RING_HYDROXYL_ALPHA     

Other Cross-Reference
    COG COG4638PR 
    EcoCyc hcaE 

Sequences
Amino acid
FASTA format 
0001 MTTPSDLNIY QLIDTQNGRV TPRIYTDPDI YQLELERIFG RCWLFLAHES QIPKPGDFFN TYMGEDAVVV 
0071 VRQKDGSIKA FLNQCRHRAM RVSYADCGNT RAFTCPYHGW SYGINGELID VPLEPRAYPQ GLCKSHWGLN 
0141 EVPCVESYKG LIFGNWDTSA PGLRDYLGDI AWYLDGMLDR REGGTEIVGG VQKWVINCNW KFPAEQFASD 
0211 QYHALFSHAS AVQVLGAKDD GSDKRLGDGQ TARPVWETAK DALQFGQDGH GSGFFFTEKP DANVWVDGAV 
0281 SSYYRETYAE AEQRLGEVRA LRLAGHNNIF PTLSWLNGTA TLRVWHPRGP DQVEVWAFCI TDKAASDEVK 
0351 AAFENSATRA FGPAGFLEQD DSENWCEIQK LLKGHRARNS KLCLEMGLGQ EKRRDDGIPG ITNYIFSETA 
0421 ARGMYQRWAD LLSSESWQEV LDKTAAYQQE VMK
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             GGTAGAAATT AACTATTTCA CATATTAGCA 
-070 ACCAACCAGC AACATCCTTA TGGCACAAAA ATAGAAGGTC AATACATCTT ATCTTTCAGG ATTAAAAAAT 
0001 atgaccacac cctcagattt gaacatttac caactgattg acacccaaaa tggtcgggtc actccgcgta 
0071 tttataccga cccggatatt taccaactgg agcttgagcg tattttcggt cgttgctggc tatttctcgc 
0141 ccacgaaagc cagatcccaa aacccggtga tttctttaac acctacatgg gagaagatgc ggttgtcgta 
0211 gtgcgtcaga aagacggcag catcaaggcg tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt 
0281 atgcagattg cggcaacact cgcgccttta cctgcccgta tcacggctgg tcttatggca ttaacggcga 
0351 gttgatcgat gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg gggactaaac 
0421 gaagttcctt gtgtggagag ttataaaggg ctaatttttg gcaactggga taccagcgca ccgggcctgc 
0491 gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt cgcgaaggcg gcaccgaaat 
0561 tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg aaattcccgg cagagcagtt cgccagtgac 
0631 cagtatcatg ctctgttcag ccatgcttct gccgttcagg tattaggggc gaaagatgat ggcagcgata 
0701 agcgcctcgg tgatggacaa accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca 
0771 ggacggtcac ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt 
0841 tcaagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc ctgcgcctgg 
0911 cgggtcataa caatattttc cccacgcttt catggctaaa cggcactgcc acactccgcg tctggcatcc 
0981 gcgcggccct gatcaagttg aagtgtgggc gttctgtatt actgacaaag ccgcctccga tgaagttaaa 
1051 gccgcttttg aaaacagcgc cactcgtgct tttggtcctg ctggttttct cgagcaggat gactcggaga 
1121 actggtgtga aatccagaaa ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg 
1191 gcttggtcag gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc 
1261 gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg ctcgataaaa 
1331 ccgccgctta ccagcaggag gtgatgaaat gaGTGCGCAA GTTTCACTAG AGTTACATCA CCGCATTAGC 
1401 CAGTTTCTCT TTCACGAAGC CAGCTTACTG GACGACTGGA AATTTCGTGA CTGGCTGGCG CA