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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
|
OCR26
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
deoD
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Related strains
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Strains Search
|
Search strains by gene name:
deoD
Search strains by all related name:
deoD b4384 ECK4376 JW4347 pup
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General information (Go to Linear View: )
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Gene Name
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deoD
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Alternative name
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b4384,ECK4376,JW4347,pup
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Location, Length
|
4,618,906 - 4,619,625
(
+
)
;
99.55
min
;
720
(bp) ,
239
(aa)
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Product
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purine-nucleoside phosphorylase
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Operon Name
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deoCABD
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Note
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GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion
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Function
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enzyme; Salvage of nucleosides and nucleotides
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Gene Ontology
|
GO:0003824
;
catalytic activity ( deoD )
GO:0004731
;
purine-nucleoside phosphorylase activity ( deoD )
GO:0005515
;
protein binding ( deoD )
GO:0005624
;
membrane fraction ( deoD )
GO:0006139
;
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process ( deoD )
GO:0009116
;
nucleoside metabolic process ( deoD )
GO:0016740
;
transferase activity ( deoD )
GO:0016757
;
transferase activity, transferring glycosyl groups ( deoD )
GO:0042278
;
purine nucleoside metabolic process ( deoD )
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PID
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1790844
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EC number
(KEGG Pathway)
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2.4.2.1
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
deoD
(
EG10222
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
DEOD_ECOLI
(
P09743
)
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- Protein name
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Purine nucleoside phosphorylase
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- Synonyms
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EC 2.4.2.1, Inosine phosphorylase, PNP
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- Gene name
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Name=deoD; Synonyms=pup; OrderedLocusNames=b4384, z5986, ECs5343;
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MMBR References
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Gene. 1982;17(3):291-8.
The cloning of the Escherichia coli K-12 deoxyribonucleoside operon.
Fischer M, Short SA.
(
6286410
)
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Mol Gen Genet. 1969;104(4):351-9.
A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli.
Ahmad SI, Pritchard RH.
(
4904508
)
|
Sov. Genet. (Engl. Transl. Genetika) 1986;22:963-972.
Genetic study of Escherichia coli K-12 mutants defective for structural and regulatory genes of second purine nucleoside phosphorylase.
Kocharyan, S. M., and M. A. Melkumyan.
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Nucleic Acids Res. 1987;15:5125-5140
Analysis of the terminator region after the deoCABD operon of Escherichia coli K-12 using a new class of single copy number operon-fusion vectors.
Larsen, J. E., B. Albrechtsen, and P. Valentin-Hansen.
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Mol Gen Genet. 1979;176(3):361-8.
Cloning the trpR gene.
Roeder W, Somerville RL.
(
160491
)
|
EMBO J. 1982;1:317-322.
The structure of tandem regulatory regions in the deo operon of Escherichia coli K12.
Valentin-Hansen, P., H. Aiba, and D. Schumperli.
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J Mol Biol. 1979;133(1):1-17.
Evidence for the existence of three promoters for the deo operon of Escherichia coli K12 in vitro.
Valentin-Hansen P, Hammer-Jespersen K, Buxton RS.
(
231107
)
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| Sequences |
Amino acid
FASTA format
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0001 MATPHINAEM GDFADVVLMP GDPLRAKYIA ETFLEDAREV NNVRGMLGFT GTYKGRKISV MGHGMGIPSC
0071 SIYTKELITD FGVKKIIRVG SCGAVLPHVK LRDVVIGMGA CTDSKVNRIR FKDHDFAAIA DFDMVRNAVD
0141 AAKALGIDAR VGNLFSADLF YSPDGEMFDV MEKYGILGVE MEAAGIYGVA AEFGAKALTI CTVSDHIRTH
0211 EQTTAAERQT TFNDMIKIAL ESVLLGDKE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ATTTTGGTAC TTCTGATATG GAATATGGCA
-070 AAGCCATGTT CTGATGGATT TGGGCGGAGC GTTGACTCCG CCTTTGTTAT GTCACAAAAA GGATAAAACA
0001 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca ggcgacccgc
0071 tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg aacaacgttc gcggtatgct
0141 gggcttcacc ggtacttaca aaggccgcaa aatttccgta atgggtcacg gtatgggtat cccgtcctgc
0211 tccatctaca ccaaagaact gatcaccgat ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg
0281 cagttctgcc gcacgtaaaa ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa
0351 ccgcatccgt tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat
0421 gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc tactctccgg
0491 acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa atggaagcgg ctggtatcta
0561 cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc tgcaccgtat ctgaccacat ccgcactcac
0631 gagcagacca ctgccgctga gcgtcagact accttcaacg acatgatcaa aatcgcactg gaatccgttc
0701 tgctgggcga taaagagtaa TTGTGTTTCG CTGCAAGGCG ATTGCCTTGT GAAGCCGGAG CGGGAGACTG
0771 CTCCGGCTTT TTAGTATCTA TTCATTTTTC TCTCCAGCTT GAATATTTTC
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