MG1655
W3110
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Gene Report : thiI
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR40 (D)  ,  OCR40-4 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  thiI
Related strains
     Strains Search Search strains by gene name:  thiI   Search strains by all related name:  thiI b0423 ECK0417 JW0413 nuvA o482 yajJ yajK

General information  (Go to Linear View:)
 Gene Name thiI  
 Alternative name b0423,ECK0417,JW0413,nuvA,o482,yajJ,yajK  
 Location, Length 440,773 - 442,221 (  +  ) ;   9.50 min ; 1449 (bp) ,   482 (aa) Go to Linear View
 Product tRNA s(4)U8 sulfurtransferase  
 Operon Name thiI  
 Note GO_process: GO:0009228 - thiamin biosynthetic process; GO_process: GO:0009451 - RNA modification  
 Function enzyme; tRNA modification  
 Gene Ontology GO:0003723 ; RNA binding ( thiI )
GO:0005737 ; cytoplasm ( thiI )
GO:0009228 ; thiamin biosynthetic process ( thiI )
 PID 1786625  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) THII_ECOLI ( P77718 )
    -  Protein name Thiamine biosynthesis protein thiI.  
    -  Synonyms  
    -  Gene name Name=thiI; OrderedLocusNames=b0423;  

 Linear View (Whole Mode)
View Location
   430.0  –  455.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP thiI (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Bacillus Anthracis Thii, A Trna- Modifying Enzyme Containing The Predicted Rna-Binding Thump Domain  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Thiamine biosynthesis protein thiI  
         PSI-BLAST GMP synthase [glutamine-hydrolyzing]
    nr     (database updated : 2007.02.20 )
         BLAST thiamine biosynthesis protein ThiI [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0301H  COG0607P 
    EcoCyc thiI 

MMBR References
Nucleic Acids Res. 1998;26:2606-2610
Identification of a gene involved in the generation of 4-thiouridine in tRNA.
Mueller, E. G., C. J. Buck, P. M. Palenchar, L. E. Barnhart, and J. L. Paulson.
J Biol Chem. 1997;272:15702-15707
Characterization of thiL, encoding thiamin-monophosphate kinase, in Salmonella typhimurium.
Webb, E., and D. Downs.

Sequences
Amino acid
FASTA format
0001 MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI EVRAKDENQR LAIRDALTRI 
0071 PGIHHILEVE DVPFTDMHDI FEKALVQYRD QLEGKTFCVR VKRRGKHDFS SIDVERYVGG GLNQHIESAR 
0141 VKLTNPDVTV HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV LSLISGGFDS GVSSYMLMRR GCRVHYCFFN 
0211 LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV AINFEPVVGE ILEKIDDGQM GVILKRMMVR AASKVAERYG 
0281 VQALVTGEAL GQVSSQTLTN LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS 
0351 PTVKAVKSKI EAEEEKFDFS ILDKVVEEAN NVDIREIAQQ TEQEVVEVET VNGFGPNDVI LDIRSIDEQE 
0421 DKPLKVEGID VVSLPFYKLS TKFGDLDQNK TWLLWCERGV MSRLQALYLR EQGFNNVKVY RP

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             TTAGTGGTAT ACTTCCGCGC CTGGATGCAG 
-070 CCGCAGGTGT GGGCTGCTGT ATTTTTCCCT ATACAAGTCG CTTAAGGCTT GCCAACGAAC CATTGCCGCC 
0001 atgaagttta tcattaaatt gttcccggaa atcaccatca aaagccaatc tgtgcgcttg cgctttataa 
0071 aaatccttac cgggaacatt cgtaacgttt taaagcacta tgatgagacg ctcgctgtcg tccgccactg 
0141 ggataacatc gaagttcgcg caaaagatga aaaccagcgt ctggctattc gcgacgctct gacccgtatt 
0211 ccgggtatcc accatattct cgaagtcgaa gacgtgccgt ttaccgacat gcacgatatt ttcgagaaag 
0281 cgttggttca gtatcgcgat cagctggaag gcaaaacctt ctgcgtacgc gtgaagcgcc gtggcaaaca 
0351 tgattttagc tcgattgatg tggaacgtta cgtcggcggc ggtttaaatc agcatattga atccgcgcgc 
0421 gtgaagctga ccaatccgga tgtgactgtc catctggaag tggaagacga tcgtctcctg ctgattaaag 
0491 gccgctacga aggtattggc ggtttcccga tcggcaccca ggaagatgtg ctgtcgctca tttccggtgg 
0561 tttcgactcc ggtgtttcca gttatatgtt gatgcgtcgc ggctgccgcg tgcattactg cttctttaac 
0631 ctcggcggcg cggcgcatga aattggcgtg cgtcaggtgg cgcattatct gtggaaccgc tttggcagct 
0701 cccaccgcgt gcgttttgtc gctattaatt tcgaaccggt cgtcggggaa attctcgaga aaatcgacga 
0771 cggtcagatg ggcgttatcc tcaaacgtat gatggtgcgt gccgcatcta aagtggctga acgttacggc 
0841 gtacaggcgc tggtcaccgg cgaagcgctc ggccaggtgt ccagccagac gctgaccaac ctgcgcctga 
0911 ttgataacgt ctccgacacg ctgatcctgc gtccgctgat ctcttacgac aaagagcaca tcatcaacct 
0981 ggcccgccag attggcaccg aagactttgc tcgcacgatg ccggaatatt gtggtgtgat ctccaaaagc 
1051 ccgacggtga aagcagttaa atcgaagatt gaagcggaag aagagaagtt cgacttcagc attctcgata 
1121 aagtggttga ggaagcgaat aacgttgata tccgcgaaat cgcccagcag accgagcagg aagtggtgga 
1191 agtggaaacc gtcaatggct tcggcccgaa cgacgtgatc ctcgatatcc gttctatcga tgaacaggaa 
1261 gataagccac tgaaagtcga agggattgat gtggtttctc tgccgttcta taaactgagc accaaatttg 
1331 gcgatctcga ccagaacaaa acctggctgc tgtggtgtga gcgcggggtg atgagccgtc tgcaggcgct 
1401 ctatctgcgc gagcagggct ttaacaatgt gaaggtatat cgcccgtaaT TTGTGGTTTT TACGTCGCAT 
1471 CTGGTCAGAT GCGACGTTTG CCGCATCCGA CACTACTCGT AATAATTATA AATCCCTGCC GCCATCACCA 
1541 GTTGTGATG