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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Gene. 1984;30(1-3):219-26.
Molecular cloning and characterization of the gene for Escherichia coli valyl-tRNA synthetase.
Skogman SG, Nilsson J.
(
6392024
)
|
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Deletion
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OCR19,20-1
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
valS
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Related strains
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Strains Search
|
Search strains by gene name:
valS
Search strains by all related name:
valS b4258 ECK4251 f951 JW4215 val-act
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General information (Go to Linear View: )
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Gene Name
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valS
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Alternative name
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b4258,ECK4251,f951,JW4215,val-act
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Location, Length
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4,479,005 - 4,481,860
(
-
)
;
96.54
min
;
2856
(bp) ,
951
(aa)
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Product
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valyl-tRNA synthetase
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Operon Name
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holC-valS
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Note
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valine tRNA synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006418 - tRNA aminoacylation for protein translation
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Function
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enzyme; Aminoacyl tRNA synthetases, tRNA modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( valS )
GO:0004812
;
aminoacyl-tRNA ligase activity ( valS )
GO:0004832
;
valine-tRNA ligase activity ( valS )
GO:0005515
;
protein binding ( valS )
GO:0005524
;
ATP binding ( valS )
GO:0005737
;
cytoplasm ( valS )
GO:0006412
;
translation ( valS )
GO:0006418
;
tRNA aminoacylation for protein translation ( valS )
GO:0006438
;
valyl-tRNA aminoacylation ( valS )
GO:0016874
;
ligase activity ( valS )
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PID
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1790708
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EC number
(KEGG Pathway)
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6.1.1.9
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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valS
(
EG11067
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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SYV_ECOLI
(
P07118
;
P78142
)
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- Protein name
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Valyl-tRNA synthetase
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- Synonyms
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EC 6.1.1.9, Valine--tRNA ligase, ValRS
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- Gene name
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Name=valS; OrderedLocusNames=b4258;
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MMBR References
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Nucleic Acids Res. 1987;15(21):9081-2.
Nucleotide sequence of Escherichia coli valyl-tRNA synthetase gene valS.
Hartlein M, Frank R, Madern D.
(
3317277
)
|
J Biol Chem. 1988;263:857-867
Valyl-tRNA synthetase gene of Escherichia coli K-12. Molecular genetic characterization.
Heck, J. D., and G. W. Hatfield.
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J Biol Chem. 1988;263:868-877
Valyl-tRNA synthetase gene of Escherichia coli K12. Primary structure and homology within a family of aminoacyl-tRNA synthetases.
Heck, J. D., and G. W. Hatfield.
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J Bacteriol. 1979;139(1):165-75.
Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase.
Baer M, Low KB, Soll D.
(
378953
)
|
Gene. 1984;30(1-3):219-26.
Molecular cloning and characterization of the gene for Escherichia coli valyl-tRNA synthetase.
Skogman SG, Nilsson J.
(
6392024
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MEKTYNPQDI EQPLYEHWEK QGYFKPNGDE SQESFCIMIP PPNVTGSLHM GHAFQQTIMD TMIRYQRMQG
0071 KNTLWQVGTD HAGIATQMVV ERKIAAEEGK TRHDYGREAF IDKIWEWKAE SGGTITRQMR RLGNSVDWER
0141 ERFTMDEGLS NAVKEVFVRL YKEDLIYRGK RLVNWDPKLR TAISDLEVEN RESKGSMWHI RYPLADGAKT
0211 ADGKDYLVVA TTRPETLLGD TGVAVNPEDP RYKDLIGKYV ILPLVNRRIP IVGDEHADME KGTGCVKITP
0281 AHDFNDYEVG KRHALPMINI LTFDGDIRES AQVFDTKGNE SDVYSSEIPA EFQKLERFAA RKAVVAAVDA
0351 LGLLEEIKPH DLTVPYGDRG GVVIEPMLTD QWYVRADVLA KPAVEAVENG DIQFVPKQYE NMYFSWMRDI
0421 QDWCISRQLW WGHRIPAWYD EAGNVYVGRN EDEVRKENNL GADVVLRQDE DVLDTWFSSA LWTFSTLGWP
0491 ENTDALRQFH PTSVMVSGFD IIFFWIARMI MMTMHFIKDE NGKPQVPFHT VYMTGLIRDD EGQKMSKSKG
0561 NVIDPLDMVD GISLPELLEK RTGNMMQPQL ADKIRKRTEK QFPNGIEPHG TDALRFTLAA LASTGRDINW
0631 DMKRLEGYRN FCNKLWNASR FVLMNTEGQD CGFNGGEMTL SLADRWILAE FNQTIKAYRE ALDSFRFDIA
0701 AGILYEFTWN QFCDWYLELT KPVMNGGTEA ELRGTRHTLV TVLEGLLRLA HPIIPFITET IWQRVKVLCG
0771 ITADTIMLQP FPQYDASQVD EAALADTEWL KQAIVAVRNI RAEMNIAPGK PLELLLRGCS ADAERRVNEN
0841 RGFLQTLARL ESITVLPADD KGPVSVTKII DGAELLIPMA GLINKEDELA RLAKEVAKIE GEISRIENKL
0911 ANEGFVARAP EAVIAKEREK LEGYAEAKAK LIEQQAVIAA L
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 acttcgttcc ttatgaagat tctctgaaac
-070 aactggcgcg cgaacgctat aaagcctacc gcgtggctgg tttcaacctg aatacggcaa cctggaaata
0001 atggaaaaga catataaccc acaagatatc gaacagccgc tttacgagca ctgggaaaag cagggctact
0071 ttaagcctaa tggcgatgaa agccaggaaa gtttctgcat catgatcccg ccgccgaacg tcaccggcag
0141 tttgcatatg ggtcacgcct tccagcaaac catcatggat accatgatcc gctatcagcg catgcagggc
0211 aaaaacaccc tgtggcaggt cggtactgac cacgccggga tcgctaccca gatggtcgtt gagcgcaaga
0281 ttgccgcaga agaaggtaaa acccgtcacg actacggccg cgaagctttc atcgacaaaa tctgggaatg
0351 gaaagcggaa tctggcggca ccattacccg tcagatgcgc cgtctcggca actccgtcga ctgggagcgt
0421 gaacgcttca ccatggacga aggcctgtcc aatgcggtga aagaagtttt cgttcgtctg tataaagaag
0491 acctgattta ccgtggcaaa cgcctggtaa actgggatcc gaaactgcgc accgctatct ctgacctgga
0561 agtcgaaaac cgcgaatcga aaggttcgat gtggcacatc cgctatccgc tggctgacgg tgcgaaaacc
0631 gcagacggta aagattatct ggtggtcgcg actacccgtc cagaaaccct gctgggcgat actggcgtag
0701 ccgttaaccc ggaagatccg cgttacaaag atctgattgg caaatatgtc attctgccgc tggttaaccg
0771 tcgtattccg atcgttggcg acgaacacgc cgacatggaa aaaggcaccg gctgcgtgaa aatcactccg
0841 gcgcacgact ttaacgacta tgaagtgggt aaacgtcacg ccctgccgat gatcaacatc ctgacctttg
0911 acggcgatat ccgtgaaagc gcccaggtgt tcgataccaa aggtaacgaa tctgacgttt attccagcga
0981 aatccctgca gagttccaga aactggagcg ttttgctgca cgtaaagcag tcgttgccgc agttgacgcg
1051 cttggcctgc tggaagaaat taaaccgcac gacctgaccg ttccttacgg cgaccgtggc ggcgtagtta
1121 tcgaaccaat gctgaccgac cagtggtacg tgcgtgccga tgtcctggcg aaaccggcgg ttgaagcggt
1191 tgagaacggc gacattcagt tcgtaccgaa gcagtacgaa aacatgtact tctcctggat gcgcgatatt
1261 caggactggt gtatctctcg tcagttgtgg tggggtcacc gtatcccggc atggtatgac gaagcgggta
1331 acgtttatgt tggccgcaac gaagacgaag tgcgtaaaga aaataacctc ggtgctgatg ttgtcctgcg
1401 tcaggacgaa gacgttctcg atacctggtt ctcttctgcg ctgtggacct tctctaccct tggctggccg
1471 gaaaataccg acgccctgcg tcagttccac ccaaccagcg tgatggtatc tggtttcgac atcattttct
1541 tctggattgc ccgcatgatc atgatgacca tgcacttcat caaagatgaa aatggcaaac cgcaggtgcc
1611 gttccacacc gtttacatga ccggcctgat tcgtgatgac gaaggccaga agatgtccaa atccaagggt
1681 aacgttatcg acccactgga tatggttgac ggtatttcgc tgccagaact gctggaaaaa cgtaccggca
1751 atatgatgca gccgcagctg gcggacaaaa tccgtaagcg caccgagaag cagttcccga acggtattga
1821 gccgcacggt actgacgcgc tgcgcttcac cctggcggcg ctggcgtcta ccggtcgtga catcaactgg
1891 gatatgaagc gtctggaagg ttaccgtaac ttctgtaaca agctgtggaa cgccagccgc tttgtgctga
1961 tgaacacaga aggtcaggat tgcggcttca acggcggcga aatgacgctg tcgctggcgg accgctggat
2031 tctggcggag ttcaaccaga ccatcaaagc gtaccgcgaa gcgctggaca gcttccgctt cgatatcgcc
2101 gcaggcattc tgtatgagtt cacctggaac cagttctgtg actggtatct cgagctgacc aagccggtaa
2171 tgaacggtgg caccgaagca gaactgcgcg gtactcgcca tacgctggtg actgtactgg aaggtctgct
2241 gcgcctcgcg catccgatca ttccgttcat caccgaaacc atctggcagc gtgtgaaagt actttgcggt
2311 atcactgccg acaccatcat gctgcagccg ttcccgcagt acgatgcatc tcaggttgat gaagccgcac
2381 tggccgacac cgaatggctg aaacaggcga tcgttgcggt acgtaacatc cgtgcagaaa tgaacatcgc
2451 gccgggcaaa ccgctggagc tgctgctgcg tggttgcagc gcggatgcag aacgtcgcgt aaatgaaaac
2521 cgtggcttcc tgcaaaccct ggcgcgtctg gaaagtatca ccgtgctgcc tgccgatgac aaaggtccgg
2591 tttccgttac gaagatcatc gacggtgcag agctgctgat cccgatggct ggcctcatca acaaagaaga
2661 tgagctggcg cgtctggcga aagaagtggc gaagattgaa ggtgaaatca gccgtatcga gaacaaactg
2731 gcgaacgaag gctttgtcgc ccgcgcaccg gaagcggtca tcgcgaaaga gcgtgagaag ctggaaggct
2801 atgcggaagc gaaagcgaaa ctgattgaac agcaggctgt tatcgccgcg ctgtaatcaa aacacagtga
2871 tgaaaacgaa ggccggagca tgctccggcc ttttttatct cttacagaaa tggtaaagaa ggcataactc
2941 cccgtgaaat actggc
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