|
PEC Original Annotations
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|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Nucleic Acids Res. 1997;25(9):1685-93.
DNA binding and DNA bending by the MelR transcription activator protein from Escherichia coli.
Bourgerie SJ, Michan CM, Thomas MS, Busby SJ, Hyde EI.
(
9108148
)
|
|
|
Deletion
|
4
(LD)
,
OCR13,15-3 (LD 4E_CRS)
(D)
,
OCR13,15-4 (LD 4E_P/O)
(D)
,
OCR15
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
melB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
melB
Search strains by all related name:
melB b4120 ECK4113 JW4081 mel-4
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General information (Go to Linear View: )
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|
Gene Name
|
melB
|
|
Alternative name
|
b4120,ECK4113,JW4081,mel-4
|
|
Location, Length
|
4,341,392 - 4,342,813
(
+
)
;
93.57
min
;
1422
(bp) ,
473
(aa)
|
|
Product
|
melibiose:sodium symporter
|
|
Operon Name
|
melAB
|
|
Note
|
melibiose permease II; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process
|
|
Function
|
transport; Transport of small molecules: Carbohydrates, organic acids, alcohols
|
|
Gene Ontology
|
GO:0005215
;
transporter activity ( melB )
GO:0005351
;
sugar:hydrogen ion symporter activity ( melB )
GO:0005886
;
plasma membrane ( melB )
GO:0006810
;
transport ( melB )
GO:0006814
;
sodium ion transport ( melB )
GO:0008643
;
carbohydrate transport ( melB )
GO:0015293
;
symporter activity ( melB )
GO:0016020
;
membrane ( melB )
GO:0016021
;
integral to membrane ( melB )
|
|
PID
|
345297180
|
EC number
(KEGG Pathway)
|
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
MELB_ECOLI
(
P02921
)
|
|
- Protein name
|
Melibiose carrier protein
|
|
- Synonyms
|
Thiomethylgalactoside permease II, Melibiose permease, Na+ (Li+)/melibiose symporter, Melibiose transporter
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- Gene name
|
Name=melB; Synonyms=mel-4; OrderedLocusNames=b4120;
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Homology Analysis
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BLAST
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|
Bacteria
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|
GTOP
|
melB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Melibiose carrier protein
|
|
PSI-BLAST
|
High-affinity glucose transporter SNF3
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
melibiose:sodium symporter [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
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PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
NA_GALACTOSIDE_SYMP
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|
MMBR References
|
J Biol Chem. 1984;259:1807-1812
Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli.
Hanatani, M., H. Yazyu, S. Shiota-Niiya, Y. Moriya, H. Kanazawa, M. Futai, and T. Tsuchiya.
|
Biochem J. 1996;318 (Pt 2):443-9.
Location of essential sequence elements at the Escherichia coli melAB promoter.
Keen J, Williams J, Busby S.
(
8809031
)
|
J Bacteriol. 1957;73(4):544-52.
Genetic control of raffinose utilization in Escherichia coli.
Lester G, Bonner DM.
(
13428689
)
|
EMBO J. 1996;15:5202-5208
In vivo membrane assembly of the E. coli polytopic protein, melibiose permease, occurs via a Sec-independent process which requires the protonmotive force.
Bassilana, M., and C. Gwizdek.
|
J Biol Chem. 1985;259:4320-4326
Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli.
Yazyu, H., S. Shiota-Niiya, T. Shimamoto, H. Kanazawa, M. Futai, and T. Tsuchiya.
|
| Sequences |
Amino acid
FASTA format
|
0001 MSISMTTKLS YGFGAFGKDF AIGIVYMYLM YYYTDVVGLS VGLVGTLFLV ARIWDAINDP IMGWIVNATR
0071 SRWGKFKPWI LIGTLANSVI LFLLFSAHLF EGTTQIVFVC VTYILWGMTY TIMDIPFWSL VPTITLDKRE
0141 REQLVPYPRF FASLAGFVTA GVTLPFVNYV GGGDRGFGFQ MFTLVLIAFF IVSTIITLRN VHEVFSSDNQ
0211 PSAEGSHLTL KAIVALIYKN DQLSCLLGMA LAYNVASNII TGFAIYYFSY VIGDADLFPY YLSYAGAANL
0281 VTLVFFPRLV KSLSRRILWA GASILPVLSC GVLLLMALMS YHNVVLIVIA GILLNVGTAL FWVLQVIMVA
0351 DIVDYGEYKL HVRCESIAYS VQTMVVKGGS AFAAFFIAVV LGMIGYVPNV EQSTQALLGM QFIMIALPTL
0421 FFMVTLILYF RFYRLNGDTL RRIQIHLLDK YRKVPPEPVH ADIPVGAVSD VKA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CGCGACTAAA CGCTACTGCG CCGGGGGATT
-070 TATTCCGGCG CACACCTCTG ACGATACCAA TAACAGAAGG CGGGCGTTGG TAACAGCGAC CCGATACCCT
0001 atgagcattt caatgactac aaaactcagt tatggatttg gagcgttcgg gaaggatttt gcgatcggca
0071 ttgtgtatat gtacctcatg tattactaca ccgatgtcgt cgggctgtct gtgggtttgg tcggtacttt
0141 gtttctggtg gcgaggatct gggatgctat taacgatccg attatgggat ggattgtaaa tgctacgcga
0211 tcgcgatggg gtaagttcaa accctggatc ctgatcggta cgttggcaaa ctctgtaatc ttatttctcc
0281 tctttagtgc gcatctgttt gaaggtacta ctcagattgt ctttgtttgc gtgacctaca tcctctgggg
0351 catgacttac accattatgg atattccctt ctggtcgctg gttccaacca tcacgctcga taaacgtgag
0421 cgcgaacaac tggttcctta tccgcgtttt tttgccagtc tggcaggctt tgttacggca ggtgtgacgc
0491 taccatttgt taattatgtc ggcggtggcg atcggggatt tggctttcag atgttcactc tggtactgat
0561 cgcctttttt attgtttcaa ccatcatcac tctgcgcaat gtgcatgaag tcttttcgtc agacaatcaa
0631 ccgtctgctg aaggaagcca tctgacactt aaagccatcg ttgcgctaat ttataaaaac gatcagcttt
0701 catgcctctt gggtatggct cttgcttata atgtagccag caacattatt accggctttg ctatctatta
0771 tttctcatat gttatcggtg atgcggattt gttcccctat tatctgtcgt atgcgggagc tgctaacctg
0841 gtgacgttag tattcttccc acgcttagtt aaatcattat cccgacgcat tttatgggcc ggagcatcta
0911 ttcttccggt gttaagctgt ggtgttctcc tgttaatggc attaatgagc tatcacaacg tcgtcctcat
0981 tgtgattgcg ggtattttgc tgaatgtggg aacggcgctt ttctgggtat tacaggtcat catggtggca
1051 gatatcgttg attacggtga atataaactg cacgtacgct gtgaaagtat cgcttactcc gtgcagacta
1121 tggtggtgaa gggcggttca gcctttgcgg cttttttcat tgcggttgtg ttagggatga ttggctatgt
1191 accgaatgtt gaacagtcta cgcaagccct attaggtatg cagtttatta tgattgctct accaactctg
1261 tttttcatgg taacgctgat tctctacttc cgtttctatc gcctcaatgg tgacacgctg cgcaggatcc
1331 agatccatct gctggataaa tatcgcaaag taccgcccga gcctgttcat gctgatattc cggtcggtgc
1401 agtgagtgat gtgaaagcct gaCGTGGACA ATAGCTAACG AAAATGGCCT GATGCGATAT GTTTATCAGG
1471 CCACGTCTTA ATGTAATGCT TTGGATTTGC AAATTTTGTA GACCGGATAA GG
|
