MG1655
W3110
Search for
Japanese | English
Top Genes Deletions Circular Linear Motif Contigs BLAST Reference SequenceCutter Download Strains TEC
Gene Report : melA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Nucleic Acids Res. 1997;25(9):1685-93.
DNA binding and DNA bending by the MelR transcription activator protein from Escherichia coli.
Bourgerie SJ, Michan CM, Thomas MS, Busby SJ, Hyde EI. ( 9108148 )
     Deletion 4 (LD)  ,  OCR13,15-3 (LD 4E_CRS) (D)  ,  OCR13,15-4 (LD 4E_P/O) (D)  ,  OCR15 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  melA
Related strains
     Strains Search Search strains by gene name:  melA   Search strains by all related name:  melA b4119 ECK4112 JW4080 mel-7

General information  (Go to Linear View:)
 Gene Name melA  
 Alternative name b4119,ECK4112,JW4080,mel-7  
 Location, Length 4,339,934 - 4,341,289 (  +  ) ;   93.54 min ; 1356 (bp) ,   451 (aa) Go to Linear View
 Product alpha-galactosidase, NAD(P)-binding  
 Operon Name melAB  
 Note alpha-galactosidase; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0009435 - NAD biosynthetic process  
 Function enzyme; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0003824 ; catalytic activity ( melA )
GO:0004553 ; hydrolase activity, hydrolyzing O-glycosyl compounds ( melA )
GO:0004557 ; alpha-galactosidase activity ( melA )
GO:0005488 ; binding ( melA )
GO:0005975 ; carbohydrate metabolic process ( melA )
GO:0008152 ; metabolic process ( melA )
GO:0016787 ; hydrolase activity ( melA )
GO:0016798 ; hydrolase activity, acting on glycosyl bonds ( melA )
GO:0030145 ; manganese ion binding ( melA )
GO:0046872 ; metal ion binding ( melA )
 PID 1790560  
 EC number
  (KEGG Pathway)   		3.2.1.22  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene melA ( EG10577 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) AGAL_ECOLI ( P06720 )
    -  Protein name Alpha-galactosidase  
    -  Synonyms EC 3.2.1.22, Melibiase  
    -  Gene name Name=melA; Synonyms=mel-7; OrderedLocusNames=b4119;  

 Linear View (Whole Mode)
View Location
   4330.0  –  4355.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP melA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Alpha-Glucosidase A, Agla, From Thermotoga Maritima In Complex With Maltose And Nad+  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Alpha-galactosidase  
         PSI-BLAST Alpha-galactosidase
    nr     (database updated : 2007.02.20 )
         BLAST alpha-galactosidase, NAD(P)-binding [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    GLYCOSYL_HYDROL_F4     

Other Cross-Reference
    COG COG1486G 
    EcoCyc melA 

MMBR References
J Biol Chem. 1984;259:1807-1812
Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli.
Hanatani, M., H. Yazyu, S. Shiota-Niiya, Y. Moriya, H. Kanazawa, M. Futai, and T. Tsuchiya.
Biochem J. 1996;318 (Pt 2):443-9.
Location of essential sequence elements at the Escherichia coli melAB promoter.
Keen J, Williams J, Busby S. ( 8809031 )
J Bacteriol. 1957;73(4):544-52.
Genetic control of raffinose utilization in Escherichia coli.
Lester G, Bonner DM. ( 13428689 )
Nucleic Acids Res. 1987;15:2213-2220
Nucleotide sequence of the melA gene, coding for alpha-galactosidase in Escherichia coli K-12.
Liljestrom, P. L., and P. Liljestrom.
Biochem Biophys Res Commun. 1984;121(1):41-6.
Nucleotide sequence of the promoter region of the melibiose operon of Escherichia coli.
Shimamoto T, Yazyu H, Futai M, Tsuchiya T. ( 6329200 )
Gene. 1987;59(2-3):253-63.
Organisation of the regulatory region of the Escherichia coli melibiose operon.
Webster C, Kempsell K, Booth I, Busby S. ( 2830169 )

Sequences
Amino acid
FASTA format 
0001 MMSAPKITFI GAGSTIFVKN ILGDVFHREA LKTAHIALMD IDPTRLEESH IVVRKLMDSA GASGKITCHT 
0071 QQKEALEDAD FVVVAFQIGG YEPCTVTDFE VCKRHGLEQT IADTLGPGGI MRALRTIPHL WQICEDMTEV 
0141 CPDATMLNYV NPMAMNTWAM YARYPHIKQV GLCHSVQGTA EELARDLNID PATLRYRCAG INHMAFYLEL 
0211 ERKTADGSYV NLYPELLAAY EAGQAPKPNI HGNTRCQNIV RYEMFKKLGY FVTESSEHFA EYTPWFIKPG 
0281 REDLIERYKV PLDEYPKRCV EQLANWHKEL EEYKKASRID IKPSREYAST IMNAIWTGEP SVIYGNVRND 
0351 GLIDNLPQGC CVEVACLVDA NGIQPTKVGT LPSHLAALMQ TNINVQTLLT EAILTENRDR VYHAAMMDPH 
0421 TAAVLGIDEI YALVDDLIAA HGDWLPGWLH R
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             GCAGGATCTG AGTTTATGGG AATGCTCAAC 
-070 CTGGAAGCCG GAGGTTTTCT GCAGATTCGC CTGCCATGAT GAAGTTATTC AAGCAAGCCA GGAGATCTGC 
0001 atgatgtctg cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat attcttggtg 
0071 atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac attgacccca cccgcctgga 
0141 agagtcgcat attgtggtgc gtaagctgat ggattcagca ggggccagcg gcaaaatcac ctgccacacc 
0211 caacagaaag aagccttaga ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt 
0281 gcacggtgac tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc 
0351 gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat gacggaagtc 
0421 tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac ctgggcgatg tatgcccgct 
0491 atccgcatat caaacaggtc gggctgtgcc attcggtgca gggaacggcg gaagagttgg cgcgtgacct 
0561 caatatcgac ccagctacgc tgcgttaccg ttgcgcaggt atcaaccata tggcgtttta cctggagctg 
0631 gagcgcaaaa ccgccgacgg cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc 
0701 aggcaccgaa gccgaatatt catggcaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa 
0771 gctgggctat ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat taagccaggt 
0841 cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc gagcagctgg 
0911 cgaactggca taaagagctg gaggagtata aaaaagcctc ccggattgat attaaaccgt cacgggaata 
0981 tgccagcaca atcatgaacg ctatctggac tggcgagccg agtgtgattt acggcaacgt ccgtaacgat 
1051 ggtttgattg ataacctgcc acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc 
1121 agccgaccaa agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac 
1191 gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat ggacccgcat 
1261 actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct gattgccgcc cacggcgact 
1331 ggctgccagg ctggttgcac cgttaaAACG CGACTAAACG CTACTGCGCC GGGGGATTTA TTCCGGCGCA 
1401 CACCTCTGAC GATACCAATA ACAGAAGGCG GGCGTTGGTA ACAGCGACCC GATACC