MG1655
W3110
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Gene Report : proP
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion 4 (LD)  ,  OCR13,15-3 (LD 4E_CRS) (D)  ,  OCR13,15-4 (LD 4E_P/O) (D)  ,  OCR14 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  proP
Related strains
     Strains Search Search strains by gene name:  proP   Search strains by all related name:  proP b4111 ECK4104 JW4072

General information  (Go to Linear View:)
 Gene Name proP  
 Alternative name b4111,ECK4104,JW4072  
 Location, Length 4,328,525 - 4,330,027 (  +  ) ;   93.29 min ; 1503 (bp) ,   500 (aa) Go to Linear View
 Product proline/glycine betaine transporter  
 Operon Name proP  
 Note low-affinity transport system; proline permease II; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006561 - proline biosynthetic process; GO_process: GO:0006970 - response to osmotic stress  
 Function transport; Transport of small molecules: Amino acids, amines  
 Gene Ontology GO:0005215; transporter activity ( proP )
GO:0005886; plasma membrane ( proP )
GO:0006810; transport ( proP )
GO:0015293; symporter activity ( proP )
GO:0016020; membrane ( proP )
GO:0016021; integral to membrane ( proP )
 PID 1790550  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) PROP_ECOLI ( P30848 )
    -  Protein name Proline/betaine transporter  
    -  Synonyms Proline porter II, PPII  
    -  Gene name Name=proP; OrderedLocusNames=b4111, z5713, ECs5093;  

 Linear View (Whole Mode)
View Location
   4320.0  –  4345.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP proP (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Lactose Permease  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Proline/betaine transporter (Proline porter II) (PPII)  
         PSI-BLAST Hexose transporter 2
    nr     (database updated : 2007.02.20 )
         BLAST low-affinity transport system; proline permease II [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    SUGAR_TRANSPORT_1     

Other Cross-Reference
    EcoCyc proP 

MMBR References
J Bacteriol. 1985;164(1):434-45.
Identification of osmoresponsive genes in Escherichia coli: evidence for participation of potassium and proline transport systems in osmoregulation.
Gowrishankar J. ( 2995318 )
J Bacteriol. 1986;166(1):331-3.
proP-mediated proline transport also plays a role in Escherichia coli osmoregulation.
Gowrishankar J. ( 3082857 )
Mol Gen Genet. 1986;205(2):225-33.
Binding protein dependent transport of glycine betaine and its osmotic regulation in Escherichia coli K12.
May G, Faatz E, Villarejo M, Bremer E. ( 2949137 )
J Bacteriol. 1995;177:144-151
Two different Escherichia coli proP promoters respond to osmotic and growth phase signals.
Mellies, J., A. Wise, and M. Villarejo.
J Bacteriol. 1980;141(3):1064-70.
Identification and mapping of a second proline permease Salmonella typhimurium.
Menzel R, Roth J. ( 6245058 )
Biochim Biophys Acta. 1995;1245(1):116-20.
Competitive accumulation of betaines by Escherichia coli K-12 and derivative strains lacking betaine porters.
Randall K, Lever M, Peddie BA, Chambers ST. ( 7654759 )
J Bacteriol. 1983;156(2):481-6.
Two proline porters in Escherichia coli K-12.
Stalmach ME, Grothe S, Wood JM. ( 6355059 )
J Bacteriol. 1995;177:5222-5231
Fis activates the RpoS-dependent stationary-phase expression of proP in Escherichia coli.
Xu, J., and R. C. Johnson.
J Mol Biol. 1993;229(1):268-76.
Isolation and sequencing of Escherichia coli gene proP reveals unusual structural features of the osmoregulatory proline/betaine transporter, ProP.
Culham DE, Lasby B, Marangoni AG, Milner JL, Steer BA, van Nues RW, Wood JM. ( 8421314 )

Sequences
Amino acid
FASTA format
0001 MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD PSVQMVAALA 
0071 TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYD TIGIWAPILL LICKMAQGFS 
0141 VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA GVVVLISTIV GEANFLDWGW RIPFFIALPL 
0211 GIIGLYLRHA LEETPAFQQH VDKLEQGDRE GLQDGPKVSF KEIATKYWRS LLTCIGLVIA TNVTYYMLLT 
0281 YMPSYLSHNL HYSEDHGVLI IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV 
0351 IGLIFAGLLM LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA GLTPTLAAWL VESSQNLMMP 
0421 AYYLMVVAVV GLITGVTMKE TANRPLKGAT PAASDIQEAK EILVEHYDNI EQKIDDIDHE IADLQAKRTR 
0491 LVQQHPRIDE 

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             GTTACAGAGA TTGCATCCTG CAATTCCCGC 
-070 TCCCCTTTTG CGGCCGTCGC GCTGATTTTT CTGGCGTTTG CGGAAATGGG CCAACTCTGC GAGGAAAGCT 
0001 atgctgaaaa ggaaaaaagt aaaaccgatt acccttcgtg atgtcaccat tattgatgac ggtaaactgc 
0071 gtaaagccat taccgcagca tcactgggta atgcaatgga atggttcgat tttggtgttt atggttttgt 
0141 tgcttacgca ttaggtaaag tttttttccc gggggctgac cccagcgtgc agatggttgc tgcacttgcc 
0211 actttctccg ttccctttct gattcgaccg cttggcggac tcttctttgg tatgttgggc gataaatatg 
0281 gtcgccagaa gatcctcgct atcactattg tgattatgtc gatcagtacg ttctgtattg gcttaatacc 
0351 gtcctacgac acgattggta tttgggcacc gattctgctg ttgatctgta agatggcaca aggtttctcg 
0421 gtcggcggtg aatataccgg ggcgtcgata tttgttgcgg aatactcccc tgaccgtaaa cgtggcttta 
0491 tgggcagctg gctggacttc ggttctattg ccgggtttgt gctgggtgcg ggcgtggtgg tgttaatttc 
0561 gaccattgtc ggcgaagcga acttcctcga ttggggctgg cgtattccgt tctttatcgc tctgccgtta 
0631 gggattatcg ggctttacct gcgccatgcg ctggaagaga ctccggcgtt ccagcagcat gtcgataaac 
0701 tggaacaggg cgaccgtgaa ggtttgcagg atggcccgaa agtctcgttt aaagagattg ccactaaata 
0771 ctggcgcagc ctgttgacat gtattggtct ggtaattgcc accaacgtga cttactacat gttgctgacc 
0841 tatatgccga gttatttgtc gcataacctg cattactccg aagaccacgg ggtgctgatt attatcgcca 
0911 ttatgatcgg tatgctgttt gtccagccgg tgatgggctt gctgagtgac cgttttggcc gtcgtccgtt 
0981 tgtgctactt ggtagtgttg ccctgtttgt gttggcgatc ccggcgttta ttctgattaa cagtaacgtc 
1051 atcggcctga tttttgccgg gttactgatg ctggcggtga tccttaactg ctttacgggc gttatggctt 
1121 ctaccttgcc agcgatgttc ccgacgcata tccgttacag cgcgctggcg gcggcattta atatttcggt 
1191 gctggttgcc ggtctgacgc caacgctggc ggcctggctg gtcgaaagct cgcagaatct gatgatgcct 
1261 gcctattacc tgatggtagt ggcggtggtt ggtttaatca ccggcgtaac catgaaagag acggcaaatc 
1331 gtccgttgaa aggtgcgaca ccggcggcgt cagatataca ggaagcgaag gaaattctcg tcgagcatta 
1401 cgataatatc gagcagaaaa tcgatgatat tgaccacgag attgccgatt tgcaggcgaa acgtacccgc 
1471 ctggtgcagc aacatccgcg aattgatgaa taaGCTGAAA CGGATGGCCT GATGTGACGC TGTCTTATCA 
1541 GGCCAATTGA ACTCTTAAGG TTCACTTAAT CTCTGACGCG CATACTCTCC TCCAGGTTAA CGG