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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
|
Proc Natl Acad Sci U S A. 1986;83(13):4650-4.
Nucleotide sequence and expression of the selenocysteine-containing polypeptide of formate dehydrogenase (formate-hydrogen-lyase-linked) from Escherichia coli.
Zinoni F, Birkmann A, Stadtman TC, Bock A.
(
2941757
)
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Deletion
|
4
(LD)
,
OCR13
(D)
,
OCR13,15-3 (LD 4E_CRS)
(D)
,
OCR13,15-4 (LD 4E_P/O)
(D)
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|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
fdhF
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
fdhF
Search strains by all related name:
fdhF b4079 ECK4072 JW4040
|
General information (Go to Linear View: )
|
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Gene Name
|
fdhF
|
|
Alternative name
|
b4079,ECK4072,JW4040
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Location, Length
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4,295,242 - 4,297,389
(
-
)
;
92.58
min
;
2148
(bp) ,
715
(aa)
|
|
Product
|
formate dehydrogenase-H, selenopolypeptide subunit
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Operon Name
|
fdhF
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Note
|
selenopolypeptide subunit of formate dehydrogenase H; GO_process: GO:0006113 - fermentation
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Function
|
enzyme; Energy metabolism, carbon: Anaerobic respiration
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Gene Ontology
|
GO:0005488
;
binding ( fdhF )
GO:0005506
;
iron ion binding ( fdhF )
GO:0006118
;
electron transport ( fdhF )
GO:0008430
;
selenium binding ( fdhF )
GO:0008863
;
formate dehydrogenase activity ( fdhF )
GO:0009326
;
formate dehydrogenase complex ( fdhF )
GO:0015942
;
formate metabolic process ( fdhF )
GO:0016491
;
oxidoreductase activity ( fdhF )
GO:0030151
;
molybdenum ion binding ( fdhF )
GO:0046872
;
metal ion binding ( fdhF )
GO:0051536
;
iron-sulfur cluster binding ( fdhF )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( fdhF )
|
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PID
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3868721
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EC number
(KEGG Pathway)
|
1.2.1.2
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|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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fdhF
(
EG10285
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
FDHF_ECOLI
(
P07658
;
P78137
)
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- Protein name
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Formate dehydrogenase H
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- Synonyms
|
EC 1.2.1.2, Formate-hydrogen-lyase-linked, selenocysteine-containing polypeptide, Formate dehydrogenase-H alpha subunit, FDH-H
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- Gene name
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Name=fdhF; OrderedLocusNames=b4079;
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MMBR References
|
Arch Microbiol. 1985;141(4):359-63.
The seleno-polypeptide of formic dehydrogenase (formate hydrogen-lyase linked) from Escherichia coli: genetic analysis.
Pecher A, Zinoni F, Bock A.
(
3160320
)
|
Arch Microbiol. 1987;148(1):44-51.
Factors affecting transcriptional regulation of the formate-hydrogen-lyase pathway of Escherichia coli.
Birkmann A, Zinoni F, Sawers G, Bock A.
(
2443100
)
|
J Bacteriol. 1995;177:4857-4864
Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli.
Rosentel, J. K., F. Healy, J. A. Maupin-Furlow, J. H. Lee, and K. T. Shanmugam.
|
J Gen Microbiol. 1987;133(9):2421-6.
Characterization of the product of the cloned fdhF gene of Escherichia coli.
Wu LF, Mandrand-Berthelot MA.
(
2834507
)
|
Mol Gen Genet. 1987;209(1):129-34.
Regulation of the fdhF gene encoding the selenopolypeptide for benzyl viologen-linked formate dehydrogenase in Escherichia coli.
Wu LF, Mandrand-Berthelot MA.
(
3118141
)
|
Proc Natl Acad Sci U S A. 1986;83:4650-4654
Nucleotide sequence and expression of the selenocysteine-containing polypeptide of formate dehydrogenase (formate-hydrogen-lyase-linked) from Escherichia coli.
Zinoni, F., A. Birkmann, T. C. Stadtman, and A. Bock.
|
Proc Natl Acad Sci U S A. 1987;84:3156-3160
Cotranslational insertion of selenocysteine into formate dehydrogenase from Escherichia coli directed by a UGA codon.
Zinoni, F., A. Birkmann, W. Leinfelder, and A. Bock.
|
Mol Microbiol. 1992;6(6):781-5.
Overproduction of a selenocysteine-containing polypeptide in Escherichia coli: the fdhF gene product.
Chen GT, Axley MJ, Hacia J, Inouye M.
(
1533438
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKKVVTVCPY CASGCKINLV VDNGKIVRAE AAQGKTNQGT LCLKGYYGWD FINDTQILTP RLKTPMIRRQ
0071 RGGKLEPVSW DEALNYVAER LSAIKEKYGP DAIQTTGSSR GTGNETNYVM QKFARAVIGT NNVDCCARVU
0141 HGPSVAGLHQ SVGNGAMSNA INEIDNTDLV FVFGYNPADS HPIVANHVIN AKRNGAKIIV CDPRKIETAR
0211 IADMHIALKN GSNIALLNAM GHVIIEENLY DKAFVASRTE GFEEYRKIVE GYTPESVEDI TGVSASEIRQ
0281 AARMYAQAKS AAILWGMGVT QFYQGVETVR SLTSLAMLTG NLGKPHAGVN PVRGQNNVQG ACDMGALPDT
0351 YPGYQYVKDP ANREKFAKAW GVESLPAHTG YRISELPHRA AHGEVRAAYI MGEDPLQTDA ELSAVRKAFE
0421 DLELVIVQDI FMTKTASAAD VILPSTSWGE HEGVFTAADR GFQRFFKAVE PKWDLKTDWQ IISEIATRMG
0491 YPMHYNNTQE IWDELRHLCP DFYGATYEKM GELGFIQWPC RDTSDADQGT SYLFKEKFDT PNGLAQFFTC
0561 DWVAPIDKLT DEYPMVLSTV REVGHYSCRS MTGNCAALAA LADEPGYAQI NTEDAKRLGI EDEALVWVHS
0631 RKGKIITRAQ VSDRPNKGAI YMTYQWWIGA CNELVTENLS PITKTPEYKY CAVRVEPIAD QRAAEQYVID
0701 EYNKLKTRLR EAALA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ccgtaatatc agggaatgac cccacataaa
-070 atgtggcata aaagatgcat actgtagtcg agagcgcgta tgcgtgattt gattaactgg agcgagaccg
0001 atgaaaaaag tcgtcacggt ttgcccctat tgcgcatcag gttgcaaaat caacctggtc gtcgataacg
0071 gcaaaatcgt ccgggcggag gcagcgcagg ggaaaaccaa ccagggtacc ctgtgtctga agggttatta
0141 tggctgggac ttcattaacg atacccagat cctgaccccg cgcctgaaaa cccccatgat ccgtcgccag
0211 cgtggcggca aactcgaacc tgtttcctgg gatgaggcac tgaattacgt tgccgagcgc ctgagcgcca
0281 tcaaagagaa gtacggtccg gatgccatcc agacgaccgg ctcctcgcgt ggtacgggta acgaaaccaa
0351 ctatgtaatg caaaaatttg cgcgcgccgt tattggtacc aataacgttg actgctgcgc tcgtgtctga
0421 cacggcccat cggttgcagg tctgcaccaa tcggtcggta atggcgcaat gagcaatgct attaacgaaa
0491 ttgataatac cgatttagtg ttcgttttcg ggtacaaccc ggcggattcc cacccaatcg tggcgaatca
0561 cgtaattaac gctaaacgta acggggcgaa aattatcgtc tgcgatccgc gcaaaattga aaccgcgcgc
0631 attgctgaca tgcacattgc actgaaaaac ggctcgaaca tcgcgctgtt gaatgcgatg ggccatgtca
0701 ttattgaaga aaatctgtac gacaaagcgt tcgtcgcttc acgtacagaa ggctttgaag agtatcgtaa
0771 aatcgttgaa ggctacacgc cggagtcggt tgaagatatc accggcgtca gcgccagtga gattcgtcag
0841 gcggcacgga tgtatgccca ggcgaaaagc gccgccatcc tgtggggcat gggtgtaacc cagttctacc
0911 agggcgtgga aaccgtgcgt tctctgacca gcctcgcgat gctgaccggt aacctcggta agccgcatgc
0981 gggtgttaac ccggttcgtg gtcagaacaa cgttcagggt gcctgcgata tgggcgcgct gccggatacg
1051 tatccgggat accagtacgt gaaagatccg gctaaccgcg agaaattcgc caaagcctgg ggcgtggaaa
1121 gcctgccagc gcataccggc tatcgcatca gcgagctgcc gcaccgcgca gcgcatggcg aagtgcgtgc
1191 cgcgtacatt atgggcgaag atccgctaca aactgacgcg gagctgtcgg cagtacgtaa agcctttgaa
1261 gatctggaac tggttatcgt tcaggacatc tttatgacca aaaccgcgtc ggcggcggat gttattttac
1331 cgtcaacgtc gtggggcgag catgaaggcg tgtttactgc ggctgaccgt ggcttccagc gtttcttcaa
1401 ggcggttgaa ccgaaatggg atctgaaaac ggactggcaa atcatcagtg aaatcgccac ccgtatgggt
1471 tatccgatgc actacaacaa cacccaggag atctgggatg agttgcgtca tctgtgcccg gatttctacg
1541 gtgcgactta cgagaaaatg ggcgaactgg gcttcattca gtggccttgc cgcgatactt cagatgccga
1611 tcaggggact tcttatctgt ttaaagagaa gtttgatacc ccgaacggtc tggcgcagtt cttcacctgc
1681 gactgggtag cgccaatcga caaactcacc gacgagtacc cgatggtact gtcaacggtg cgtgaagttg
1751 gtcactactc ttgccgttcg atgaccggta actgtgcggc actggcggcg ctggctgatg aacctggcta
1821 cgcacaaatc aataccgaag acgccaaacg tctgggtatt gaagatgagg cattggtttg ggtgcactcg
1891 cgtaaaggca aaattatcac ccgtgcgcag gtcagcgatc gtccgaacaa aggggcgatt tacatgacct
1961 accagtggtg gattggtgcc tgtaacgagc tggttaccga aaacttaagc ccgattacga aaacgccgga
2031 gtacaaatac tgcgccgttc gcgtcgagcc gatcgccgat cagcgcgccg ccgagcagta cgtgattgac
2101 gagtacaaca agttgaaaac tcgcctgcgc gaagcggcac tggcgtaata ccgtcctttc tacagcctcc
2171 tttcggaggc tgttttttta tccattcgaa ctctttatac tggttactcc ctacccaatc gtattatcaa
2241 aatgaaaa
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