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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1973;115(3):752-61.
Synthesis of unmethylated deoxyribonucleic acid in a dnaB temperature-sensitive mutant of Escherichia coli starved for methionine.
Bouche JC.
(
4580566
)
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Deletion
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OCR12-18
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
dnaB
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Related strains
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Strains Search
|
Search strains by gene name:
dnaB
Search strains by all related name:
dnaB b4052 ECK4044 groP grpA grpD JW4012
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General information (Go to Linear View: )
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Gene Name
|
dnaB
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Alternative name
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b4052,ECK4044,groP,grpA,grpD,JW4012
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Location, Length
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4,262,337 - 4,263,752
(
+
)
;
91.87
min
;
1416
(bp) ,
471
(aa)
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Product
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replicative DNA helicase
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Operon Name
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dnaB
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Note
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replicative DNA helicase; part of primosome; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication
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Function
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factor; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( dnaB )
GO:0003677
;
DNA binding ( dnaB )
GO:0003678
;
DNA helicase activity ( dnaB )
GO:0004386
;
helicase activity ( dnaB )
GO:0005524
;
ATP binding ( dnaB )
GO:0005658
;
alpha DNA polymerase:primase complex ( dnaB )
GO:0006260
;
DNA replication ( dnaB )
GO:0006269
;
DNA replication, synthesis of RNA primer ( dnaB )
GO:0016787
;
hydrolase activity ( dnaB )
GO:0017111
;
nucleoside-triphosphatase activity ( dnaB )
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PID
|
1790486
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EC number
(KEGG Pathway)
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3.6.1.-
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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dnaB
(
EG10236
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
4
more]
)
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Entry name(Acc.no)
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DNAB_ECOLI
(
P03005
)
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- Protein name
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Replicative DNA helicase
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- Synonyms
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EC 3.6.1.-
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- Gene name
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OrderedLocusNames=b4052, SF4154, S3577;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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dnaB
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Crystal Structure Of The Helicase Domain Of The Gene4 Protein Of Bacteriophage T7
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Replicative DNA helicase
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PSI-BLAST
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Ribose import ATP-binding protein rbsA
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nr
(database updated :
2007.02.20
)
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BLAST
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replicative DNA helicase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
ATP_GTP_A
LEUCINE_ZIPPER
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MMBR References
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Proc Natl Acad Sci U S A. 1971;68:2977-2981
Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase.
Georgopoulos, C.
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Mol Gen Genet. 1995;248(6):695-702.
Expression of the dnaB gene of Escherichia coli is inducible by replication-blocking DNA damage in a recA-independent manner.
Kleinsteuber S, Quinones A.
(
7476872
)
|
J Biol Chem. 1980;255:9413-9420
Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycerol-3-phosphate acyltransferase.
Lightner, V. A., T. J. Larsen, P. Tailleur, G. D. Kantor, C. R. H. Raetz, R. M. Bell, and P. Modrich.
|
J Biol Chem. 1984;259:97-101
Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli.
Nakayama, N., N. Arai, M. W. Bond, Y. Kaziro, and K.-I. Arai.
|
Eur J Biochem. 1973;35(2):331-7.
Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12.
Paetz W, Nass G.
(
4577856
)
|
J Mol Biol. 1977;113(1):1-25.
Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12.
Saito H, Uchida H.
(
328896
)
|
Genetics 1981;98:677-689
High frequency of genetic duplications in the dnaB region of the Escherichia coli K12 chromosome.
Sclafani, R. A., and J. A. Wechsler.
|
J Biol Chem. 1996;271:21398-21405
The interaction between helicase and primase sets the replication fork clock.
Tougu, K., and K. J. Marians.
|
Mol Gen Genet. 1996;252(6):755-60.
The grpD55 locus of Escherichia coli appears to be an allele of dnaB.
Bull HJ, Hayes S.
(
8917321
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAGNKPFNKQ QAEPRERDPQ VAGLKVPPHS IEAEQSVLGG LMLDNERWDD VAERVVADDF YTRPHRHIFT
0071 EMARLQESGS PIDLITLAES LERQGQLDSV GGFAYLAELS KNTPSAANIS AYADIVRERA VVREMISVAN
0141 EIAEAGFDPQ GRTSEDLLDL AESRVFKIAE SRANKDEGPK NIADVLDATV ARIEQLFQQP HDGVTGVNTG
0211 YDDLNKKTAG LQPSDLIIVA ARPSMGKTTF AMNLVENAAM LQDKPVLIFS LEMPSEQIMM RSLASLSRVD
0281 QTKIRTGQLD DEDWARISGT MGILLEKRNI YIDDSSGLTP TEVRSRARRI AREHGGIGLI MIDYLQLMRV
0351 PALSDNRTLE IAEISRSLKA LAKELNVPVV ALSQLNRSLE QRADKRPVNS DLRESGSIEQ DADLIMFIYR
0421 DEVYHENSDL KGIAEIIIGK QRNGPIGTVR LTFNGQWSRF DNYAGPQYDD E
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TTCAATTCGT GTTGCCATGT GTCCTCCAGA
-070 ACGTATCGTC AGGGTCTGCT TCATATGATA AAGTTTCGAC CCATTCTTTA TCTCGGTAAC TCCATTCACT
0001 atggcaggaa ataaaccctt caacaaacag caggctgaac cccgcgaacg cgatccacaa gttgccgggc
0071 tgaaagtgcc tccgcactcg atcgaagcgg agcagtcggt gttgggcggt ttaatgctag ataacgaacg
0141 ctgggatgat gtagccgagc gtgtggtagc agacgatttt tacacccgcc cacaccgtca tatctttact
0211 gaaatggcgc gtttgcagga aagcggtagc cctatcgatc tgattactct tgcggaatcg ctggaacgcc
0281 aggggcaact cgatagcgtc ggtggttttg cttatctggc agagctgtca aaaaatacgc caagtgcggc
0351 taacatcagt gcctatgcgg acatcgtgcg tgaacgtgcc gttgtccgtg agatgatctc ggttgcgaat
0421 gagattgccg aagctggttt tgatccgcag gggcgtacca gcgaagatct gctggatctg gctgaatccc
0491 gcgtctttaa aattgccgaa agtcgtgcga acaaagacga agggccgaag aacatcgccg atgtgctcga
0561 cgcaaccgtg gcgcgtattg agcagttgtt tcagcagcca cacgatggcg ttaccggggt aaacaccggt
0631 tatgacgatc tcaacaaaaa aaccgctggc ttgcagccgt cggatttgat catcgtcgcc gcgcgtccgt
0701 cgatgggtaa aacaacattt gcgatgaacc tcgtcgaaaa cgcggcgatg ttgcaggata aaccggtact
0771 tatcttctcg ctggagatgc catcagaaca gatcatgatg cgttctctgg cgtcgctgtc gcgcgttgac
0841 cagactaaaa tccgtaccgg gcagctcgat gacgaagact gggcgcgcat ttccggcacc atgggtattt
0911 tgctcgaaaa acgcaatatc tatatcgatg actcctccgg cctgacgcca acggaagtgc gttcccgcgc
0981 acgccgtatt gcccgtgaac acggcggcat cgggcttatc atgatcgact acctgcaact gatgcgcgta
1051 ccggcgcttt ccgataaccg tacgctggaa attgcagaaa tctctcgctc gctgaaagca ctggcgaaag
1121 aactgaacgt gccggtggtg gcgctgtccc agttgaaccg ttctctggaa caacgtgccg acaaacgccc
1191 ggtcaactcc gacctgcgtg aatctggctc tatcgagcag gatgcggact tgatcatgtt tatctatcgt
1261 gatgaggtgt atcacgaaaa cagtgattta aaaggcatcg cggaaattat tatcggtaaa caacgtaacg
1331 gcccaatcgg gacggtacgc ctgaccttta acggtcaatg gtcgcgcttc gacaactatg cggggccgca
1401 gtacgacgac gaataaTAAT TATTTTATGA ATTAGGTAAT TAAAGCAAAC ACTTATCAAG GAACACAAAT
1471 GCAAGCGGCA ACTGTTGTGA TTAACCGCCG CGCTCTGCGA CACAAC
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