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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCR12
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
lexA
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Related strains
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Strains Search
|
Search strains by gene name:
lexA
Search strains by all related name:
lexA b4043 ECK4035 exrA JW4003 o202b spr tsl umuA
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General information (Go to Linear View: )
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Gene Name
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lexA
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Alternative name
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b4043,ECK4035,exrA,JW4003,o202b,spr,tsl,umuA
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Location, Length
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4,255,138 - 4,255,746
(
+
)
;
91.71
min
;
609
(bp) ,
202
(aa)
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Product
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DNA-binding transcriptional repressor of SOS regulon
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Operon Name
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lexA-dinF
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Note
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regulator for SOS(lexA) regulon; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0006281 - DNA repair; GO_process: GO:0006350 - transcription; GO_process: GO:0009432 - SOS response
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Function
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regulator; Global regulatory functions
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Gene Ontology
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GO:0003677
;
DNA binding ( lexA )
GO:0005515
;
protein binding ( lexA )
GO:0005886
;
plasma membrane ( spr )
GO:0006260
;
DNA replication ( lexA )
GO:0006281
;
DNA repair ( lexA )
GO:0006350
;
transcription ( lexA )
GO:0006355
;
regulation of transcription, DNA-dependent ( lexA )
GO:0006508
;
proteolysis ( lexA )
GO:0006974
;
response to DNA damage stimulus ( lexA )
GO:0008992
;
repressor LexA activity ( lexA )
GO:0009432
;
SOS response ( lexA )
GO:0016020
;
membrane ( spr )
GO:0016787
;
hydrolase activity ( lexA )
GO:0045449
;
regulation of transcription ( lexA )
GO:0045892
;
negative regulation of transcription, DNA-dependent ( lexA )
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PID
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1790476
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EC number
(KEGG Pathway)
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3.4.21.88
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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LEXA_ECOLI
(
P03033
)
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- Protein name
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LexA repressor
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- Synonyms
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EC 3.4.21.88
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- Gene name
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OrderedLocusNames=b4043, c5014, z5642, ECs5026, SF4162, S3569;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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spr
(
homologous genes of other bacterias
)
lexA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Lexa S119a Mutant
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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LexA repressor
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PSI-BLAST
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LexA repressor
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nr
(database updated :
2007.02.20
)
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BLAST
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LexA [unidentified cloning vector]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
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MMBR References
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J Biol Chem. 1996;271:23874-23883
Interaction of Escherichia coli RecA protein with LexA repressor. II. Inhibition of DNA strand exchange by the uncleavable LexA S119A repressor argues that recombination and SOS induction are competitive processes.
Harmon, F. G., W. M. Rehrauer, and S. C. Kowalczykowski.
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J Bacteriol. 1997;179:1931-1939
sfi-independent filamentation in Escherichia coli is lexA dependent and requires DNA damage for induction.
Hill, T. M., B. Sharma, M. Valjavec-Gratian, and J. Smith.
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Cell. 1981;23(3):689-97.
Nucleotide sequence of the lexA gene of E. coli.
Horii T, Ogawa T, Ogawa H.
(
7013987
)
|
Proc Natl Acad Sci U S A. 1981;78:4199-4203
Purified lexA protein is a repressor of the recA and lexA genes.
Little, J. W., D. W. Mount, and C. R. Yanisch-Perron.
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Gene. 1980;10(3):237-47.
Isolation of recombinant plasmids and phage carrying the lexA gene of Escherichia coli K-12.
Little JW.
(
6254842
)
|
Nucleic Acids Res. 1981;9:4149-4161
Nucleotide sequence of the lexA gene of Escherichia coli K-12.
Markham, B. E., J. W. Little, and D. W. Mount.
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Nucleic Acids Res. 1981;9:529-543
Organization of the lexA gene of Escherichia coli and nucleotide sequence of the regulatory region.
Miki, T., Y. Ebina, F. Kishi, and A. Nakazawa.
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Proc Natl Acad Sci U S A. 1996;93:11528-11533
Mutant LexA proteins with specific defects in autodigestion.
Shepley, D. P., and J. W. Little.
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Gene. 1982;18(1):77-85.
Effect of lexA and ssb genes, present on a uvrA recombinant plasmid, on the UV survival of Escherichia coli K-12.
Brandsma JA, Stoorvogel J, van Sluis CA, van de Putte P.
(
6286420
)
|
Proc Natl Acad Sci U S A. 1980;77:1932-1936
The lexA gene product represses its own promoter.
Brent, R., and M. Ptashne.
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Proc Natl Acad Sci U S A. 1981;78:4204-4208
Mechanism of action of the lexA gene product.
Brent, R., and M. Ptashne.
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| Sequences |
Amino acid
FASTA format
|
0001 MKALTARQQE VFDLIRDHIS QTGMPPTRAE IAQRLGFRSP NAAEEHLKAL ARKGVIEIVS GASRGIRLLQ
0071 EEEEGLPLVG RVAAGEPLLA QQHIEGHYQV DPSLFKPNAD FLLRVSGMSM KDIGIMDGDL LAVHKTQDVR
0141 NGQVVVARID DEVTVKRLKK QGNKVELLPE NSEFKPIVVD LRQQSFTIEG LAVGVIRNGD WL
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AATTCGATAA ATCTCTGGTT TATTGTGCAG
-070 TTTATGGTTC CAAAATCGCC TTTTGCTGTA TATACTCACA GCATAACTGT ATATACACCC AGGGGGCGGA
0001 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
0071 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
0141 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg tctgttgcag
0211 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc acttctggcg caacagcata
0281 ttgaaggtca ttatcaggtc gatccttcct tattcaagcc gaatgctgat ttcctgctgc gcgtcagcgg
0351 gatgtcgatg aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt
0421 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata
0491 aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
0561 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaaC ATATCTCTGA GACCGCGATG
0631 CCGCCTGGCG TCGCGGTTTG TTTTTCATCT CTCTTCATCA GGCTTGTCTG CATGGCATTC CTCACTTCAT
0701 CTGATAAAG
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