MG1655
W3110
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Gene Report : dgkA
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR12 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  dgkA
Related strains
     Strains Search Search strains by gene name:  dgkA   Search strains by all related name:  dgkA b4042 ECK4034 JW4002

General information  (Go to Linear View:)
 Gene Name dgkA  
 Alternative name b4042,ECK4034,JW4002  
 Location, Length 4,254,660 - 4,255,028 (  +  ) ;   91.70 min ; 369 (bp) ,   122 (aa) Go to Linear View
 Product diacylglycerol kinase  
 Operon Name dgkA  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0008654 - phospholipid biosynthetic process  
 Function enzyme; Fatty acid and phosphatidic acid biosynthesis  
 Gene Ontology GO:0004143 ; diacylglycerol kinase activity ( dgkA )
GO:0005886 ; plasma membrane ( dgkA )
GO:0008654 ; phospholipid biosynthetic process ( dgkA )
GO:0016020 ; membrane ( dgkA )
GO:0016021 ; integral to membrane ( dgkA )
GO:0016301 ; kinase activity ( dgkA )
GO:0016740 ; transferase activity ( dgkA )
 PID 1790475  
 EC number
  (KEGG Pathway)
 
2.7.1.107  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene dgkA ( EG10224 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) KDGL_ECOLI ( P00556 )
    -  Protein name Diacylglycerol kinase  
    -  Synonyms EC 2.7.1.107, DAGK, Diglyceride kinase, DGK  
    -  Gene name OrderedLocusNames=b4042, c5013, z5641, ECs5025, SF4163, S3568;  

 Linear View (Whole Mode)
View Location
   4245.0  –  4270.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP dgkA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
        PSI-BLAST  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Diacylglycerol kinase (DAGK) (Diglyceride kinase) (DGK)  
         PSI-BLAST Diacylglycerol kinase (DAGK) (Diglyceride kinase) (DGK)
    nr     (database updated : 2007.02.20 )
         BLAST diacylglycerol kinase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    DAGK_PROKAR     

Other Cross-Reference
    EcoCyc dgkA 

MMBR References
J Biol Chem. 1983;258:10856-10861
The DNA sequences encoding plsB and dgk loci of Escherichia coli.
Lightner, V. A., R. M. Bell, and P. Modrich.
J Biol Chem. 1980;255:9413-9420
Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycerol-3-phosphate acyltransferase.
Lightner, V. A., T. J. Larsen, P. Tailleur, G. D. Kantor, C. R. H. Raetz, R. M. Bell, and P. Modrich.

Sequences
Amino acid
FASTA format
0001 MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDAITRVLLI SSVMLVMIVE 
0071 ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIVAV ITWCILLWSH FG

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             GTGGTATCCG CTCATGTTTC GCGCGGCGCT 
-070 ACGCAAACCC GAATCATCGG ATTTAACGGT ACACTGATAT TGACGCTCAT AATGTAAAAA GGTTCTTTCA 
0001 atggccaata ataccactgg attcacccga attatcaaag ctgctggcta ttcctggaaa ggtttacgcg 
0071 ctgcatggat caacgaagcg gcattccgtc aggaaggcgt agcggtattg ttggcggtgg tcatcgcctg 
0141 ctggctggat gtggacgcga ttacccgcgt gctgcttatc agctccgtga tgctggtgat gattgtggaa 
0211 atcctcaata gcgccatcga agcagtggtt gaccgaattg gctctgaata ccatgagctt tccggacgcg 
0281 caaaagatat gggatccgct gcggtgctga ttgccattat cgtcgccgtg attacctggt gcattctgtt 
0351 atggtcgcat tttggataaC CCTTCCAGAA TTCGATAAAT CTCTGGTTTA TTGTGCAGTT TATGGTTCCA 
0421 AAATCGCCTT TTGCTGTATA TACTCACAGC ATAACTGTAT ATACACCCA