MG1655
W3110
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Gene Report : malK
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR12 (D)  ,  DE(xylE-malB)262  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  malK
Related strains
     Strains Search Search strains by gene name:  malK   Search strains by all related name:  malK b4035 ECK4027 JW3995 o371

General information  (Go to Linear View:)
 Gene Name malK  
 Alternative name b4035,ECK4027,JW3995,o371  
 Location, Length 4,244,807 - 4,245,922 (  +  ) ;   91.49 min ; 1116 (bp) ,   371 (aa) Go to Linear View
 Product fused maltose transport subunit, ATP-bindingcomponent of ABC superfamily/regulatory protein  
 Operon Name malK-lamB-malM  
 Note ATP-binding component of transport system for maltose; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0000166 ; nucleotide binding ( malK )
GO:0005215 ; transporter activity ( malK )
GO:0005351 ; sugar:hydrogen ion symporter activity ( malK )
GO:0005524 ; ATP binding ( malK )
GO:0005886 ; plasma membrane ( malK )
GO:0006810 ; transport ( malK )
GO:0008643 ; carbohydrate transport ( malK )
GO:0015423 ; maltose-transporting ATPase activity ( malK )
GO:0015609 ; maltooligosaccharide-importing ATPase activity ( malK )
GO:0016020 ; membrane ( malK )
GO:0016787 ; hydrolase activity ( malK )
GO:0016820 ; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances ( malK )
GO:0016887 ; ATPase activity ( malK )
GO:0017111 ; nucleoside-triphosphatase activity ( malK )
GO:0043190 ; ATP-binding cassette (ABC) transporter complex ( malK )
 PID 1790467  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) MALK_ECOLI ( P02914 ; Q47348 )
    -  Protein name Maltose/maltodextrin transport ATP-binding protein malK.  
    -  Synonyms  
    -  Gene name Name=malK; OrderedLocusNames=b4035, z5633, ECs5018;  

 Linear View (Whole Mode)
View Location
   4235.0  –  4260.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP malK (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of The Atp-Bound E. Coli Malk  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Maltose/maltodextrin import ATP-binding protein malK  
         PSI-BLAST Maltose/maltodextrin import ATP-binding protein malK
    nr     (database updated : 2007.02.20 )
         BLAST maltose/maltodextrin transport ATP-binding protein malK [Escherichia coli UTI89]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A    ABC_TRANSPORTER     

Other Cross-Reference
    COG COG3839G 
    EcoCyc malK 

MMBR References
J Bacteriol. 1980;144(1):7-11.
Methyl-alpha-maltoside and 5-thiomaltose: analogs transported by the Escherichia coli maltose transport system.
Ferenci T. ( 6998971 )
Nucleic Acids Res. 1982;10:7449-7458
Sequence of the malK gene in E. coli K12.
Gilson, E., H. Nikaido, and M. Hofnung.
J Bacteriol. 1993;175:6546-6552
Functional exchangeability of the ABC proteins of the periplasmic binding protein-dependent transport systems Ugp and Mal of Escherichia coli.
Hekstra, D., and J. Tommassen.
J Mol Biol. 1983;170(4):861-82.
Mutations in the promoter regions of the malEFG and malK-lamB operons of Escherichia coli K12.
Bedouelle H. ( 6417341 )
Mol Gen Genet. 1982;185(1):82-7.
A DNA sequence containing the control regions of the malEFG and malK-lamB operons in Escherichia coli K12.
Bedouelle H, Hofnung M. ( 6283312 )
J Mol Biol. 1982;161(4):519-31.
Promoters of the malEFG and malK-lamB operons in Escherichia coli K12.
Bedouelle H, Schmeissner U, Hofnung M, Rosenberg L. ( 6185687 )
J. Biochem. (Tokyo) 1983;94:243-247
Nucleotide sequence of the regulatory region of malB operons in E. coli.
Ohsumi, M., T. Sekiya, S. Nishimura, and M. Ohki.
J Bacteriol. 1978;133(3):1358-67.
Permease-specific mutations in Salmonella typhimurium and Escherichia coli that release the glycerol, maltose, melibiose, and lactose transport systems from regulation by the phosphoenolpyruvate:sugar phosphotransferase system.
Saier MH Jr, Straud H, Massman LS, Judice JJ, Newman MJ, Feucht BU. ( 346569 )
Genetics 1972;71:207-216
On some genetic aspects of phage lambda resistance in E. coli K12.
Thirion, J. P., and M. Hofnung.
Mol Gen Genet. 1981;184(3):479-83.
rho Mutations restore lamB expression in E. coli K12 strains with an inactive malB region.
Colonna B, Hofnung M. ( 6278260 )

Sequences
Amino acid
FASTA format
0001 MASVQLQNVT KAWGEVVVSK DINLDIHEGE FVVFVGPSGC GKSTLLRMIA GLETITSGDL FIGEKRMNDT 
0071 PPAERGVGMV FQSYALYPHL SVAENMSFGL KLAGAKKEVI NQRVNQVAEV LQLAHLLDRK PKALSGGQRQ 
0141 RVAIGRTLVA EPSVFLLDEP LSNLDAALRV QMRIEISRLH KRLGRTMIYV THDQVEAMTL ADKIVVLDAG 
0211 RVAQVGKPLE LYHYPADRFV AGFIGSPKMN FLPVKVTATA IDQVQVELPM PNRQQVWLPV ESRDVQVGAN 
0281 MSLGIRPEHL LPSDIADVIL EGEVQVVEQL GNETQIHIQI PSIRQNLVYR QNDVVLVEEG ATFAIGLPPE 
0351 RCHLFREDGT ACRRLHKEPG V

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             AAGCCAGGGG GTGGAGGATT TAAGCCATCT 
-070 CCTGATGACG CATAGTCAGC CCATCATGAA TGTTGCTGTC GATGACAGGT TGTTACAAAG GGAGAAGGGC 
0001 atggcgagcg tacagctgca aaatgtaacg aaagcctggg gcgaggtcgt ggtatcgaaa gatatcaatc 
0071 tcgatatcca tgaaggtgaa ttcgtggtgt ttgtcggacc gtctggctgc ggtaaatcga ctttactgcg 
0141 catgattgcc gggcttgaga cgatcaccag cggcgacctg ttcatcggtg agaaacggat gaatgacact 
0211 ccgccagcag aacgcggcgt tggtatggtg tttcagtctt acgcgctcta tccccacctg tcagtagcag 
0281 aaaacatgtc atttggcctg aaactggctg gcgcaaaaaa agaggtgatt aaccaacgcg ttaaccaggt 
0351 ggcggaagtg ctacaactgg cgcatttgct ggatcgcaaa ccgaaagcgc tctccggtgg tcagcgtcag 
0421 cgtgtggcga ttggccgtac gctggtggcc gagccaagcg tatttttgct cgatgaaccg ctctccaacc 
0491 tcgatgctgc actgcgtgtg caaatgcgta tcgaaatctc ccgtctgcat aaacgcctgg gccgcacaat 
0561 gatttacgtc acccacgatc aggtcgaagc gatgacgctg gccgacaaaa tcgtggtgct ggacgccggt 
0631 cgcgtggcgc aggttgggaa accgctggag ctgtaccact atccggcaga ccgttttgtc gccggattta 
0701 tcggttcgcc aaagatgaac ttcctgccgg taaaagtgac cgccaccgca atcgatcaag tgcaggtgga 
0771 gctgccgatg ccaaatcgtc agcaagtctg gctgccagtt gaaagccgtg atgtccaggt tggagccaat 
0841 atgtcgctgg gtattcgccc ggaacatcta ctgccgagtg atatcgctga cgtcatcctt gagggtgaag 
0911 ttcaggtcgt cgagcaactc ggcaacgaaa ctcaaatcca tatccagatc ccttccattc gtcaaaacct 
0981 ggtgtaccgc cagaacgacg tggtgttggt agaagaaggt gccacattcg ctatcggcct gccgccagag 
1051 cgttgccatc tgttccgtga ggatggcact gcatgtcgtc gactgcataa ggagccgggc gtttaaGCAC 
1121 CCCACAAAAC ACACAAAGCC TGTCACAGGT GATGTGAAAA AAGAAAAGCA ATGACTCAGG AGATAGAATG 
1191 ATGATTACTC TGCGCAAACT TCCTCT