MG1655
W3110
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Gene Report : malE
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR12 (D)  ,  DE(xylE-malB)262  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  malE
Related strains
     Strains Search Search strains by gene name:  malE   Search strains by all related name:  malE b4034 ECK4026 f396 JW3994 malJ

General information  (Go to Linear View:)
 Gene Name malE  
 Alternative name b4034,ECK4026,f396,JW3994,malJ  
 Location, Length 4,243,252 - 4,244,442 (  -  ) ;   91.46 min ; 1191 (bp) ,   396 (aa) Go to Linear View
 Product maltose transporter subunit  
 Operon Name malEFG  
 Note periplasmic maltose-binding protein; substrate recognition for transport and chemotaxis; periplasmic-binding component of ABC superfamily; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0006457 - protein folding  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0005215 ; transporter activity ( malE )
GO:0005351 ; sugar:hydrogen ion symporter activity ( malE )
GO:0005363 ; maltose transmembrane transporter activity ( malE )
GO:0006810 ; transport ( malE )
GO:0008643 ; carbohydrate transport ( malE )
GO:0015768 ; maltose transport ( malE )
GO:0042597 ; periplasmic space ( malE )
 PID 1790466  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene malE ( EG10554 )  
    Number of removed 26 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) MALE_ECOLI ( P02928 )
    -  Protein name Maltose-binding periplasmic protein precursor  
    -  Synonyms Maltodextrin-binding protein, MMBP  
    -  Gene name Name=malE; OrderedLocusNames=b4034, z5632, ECs5017;  

 Linear View (Whole Mode)
View Location
   4230.0  –  4255.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP malE (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain P, The Crystal Structure Of The Argonaute2 Paz Domain (As A Mbp Fusion)  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Maltose-binding periplasmic protein precursor (Maltodextrin-binding protein) (MMBP)  
         PSI-BLAST Maltose-binding periplasmic protein precursor (Maltodextrin-binding protein) (MMBP)
    nr     (database updated : 2007.02.20 )
         BLAST periplasmic maltose-binding protein [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    PROKAR_LIPOPROTEIN    SBP_BACTERIAL_1     

Other Cross-Reference
    COG COG2182G 
    EcoCyc malE 

MMBR References
J Biol Chem. 1984;259:10606-10613
Sequence of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12.
Duplay, P., H. Bedouelle, A. Fowler, I. Zabin, W. Saurin, and M. Hofnung.
J Bacteriol. 1980;144(1):7-11.
Methyl-alpha-maltoside and 5-thiomaltose: analogs transported by the Escherichia coli maltose transport system.
Ferenci T. ( 6998971 )
J Bacteriol. 1993;175:4036-4044
prlA supression of defective export of maltose-binding protein in secB mutants of Escherichia coli.
Francetic, O., M. P. Hanson, and C. A. Kumamoto.
J Mol Biol. 1983;170(4):861-82.
Mutations in the promoter regions of the malEFG and malK-lamB operons of Escherichia coli K12.
Bedouelle H. ( 6417341 )
Mol Gen Genet. 1982;185(1):82-7.
A DNA sequence containing the control regions of the malEFG and malK-lamB operons in Escherichia coli K12.
Bedouelle H, Hofnung M. ( 6283312 )
Nature. 1980;285(5760):78-81.
Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli.
Bedouelle H, Bassford PJ Jr, Fowler AV, Zabin I, Beckwith JR, Hofnung M. ( 6990274 )
J Mol Biol. 1982;161(4):519-31.
Promoters of the malEFG and malK-lamB operons in Escherichia coli K12.
Bedouelle H, Schmeissner U, Hofnung M, Rosenberg L. ( 6185687 )
J. Biochem. (Tokyo) 1983;94:243-247
Nucleotide sequence of the regulatory region of malB operons in E. coli.
Ohsumi, M., T. Sekiya, S. Nishimura, and M. Ohki.
J Biol Chem. 1996;271:8046-8052
Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies.
Betton, J., and M. Hofnung.
J Mol Biol. 1996;262(2):140-50.
Probing the structural role of an alpha beta loop of maltose-binding protein by mutagenesis: heat-shock induction by loop variants of the maltose-binding protein that form periplasmic inclusion bodies.
Betton JM, Boscus D, Missiakas D, Raina S, Hofnung M. ( 8831785 )
J Bacteriol. 1977;132(1):166-73.
Xylitol and D-arabitol toxicities due to derepressed fructose, galactitol, and sorbitol phosphotransferases of Escherichia coli.
Reiner AM. ( 334721 )
J Mol Biol. 1996;264(5):852-62.
On the role of the multiple regulatory elements involved in the activation of the Escherichia coli malEp promoter.
Richet E. ( 9000616 )
J Bacteriol. 1989;171(9):4640-7.
Mutations that improve export of maltose-binding protein in SecB- cells of Escherichia coli.
Collier DN, Bassford PJ Jr. ( 2670890 )

Sequences
Amino acid
FASTA format
0001 MKIKTGARIL ALSALTTMMF SASALAKIEE GKLVIWINGD KGYNGLAEVG KKFEKDTGIK VTVEHPDKLE 
0071 EKFPQVAATG DGPDIIFWAH DRFGGYAQSG LLAEITPDKA FQDKLYPFTW DAVRYNGKLI AYPIAVEALS 
0141 LIYNKDLLPN PPKTWEEIPA LDKELKAKGK SALMFNLQEP YFTWPLIAAD GGYAFKYENG KYDIKDVGVD 
0211 NAGAKAGLTF LVDLIKNKHM NADTDYSIAE AAFNKGETAM TINGPWAWSN IDTSKVNYGV TVLPTFKGQP 
0281 SKPFVGVLSA GINAASPNKE LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA 
0351 QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QTRITK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gggcgtaggg gcaaggagga tggaaagagg 
-070 ttgccgtata aagaaactag agtccgttta ggtgttttca cgagcacttc accaacaagg accatagatt 
0001 atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt tccgcctcgg 
0071 ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc 
0141 tgaagtcggt aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa 
0211 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac gaccgctttg 
0281 gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc 
0351 gtttacctgg gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg 
0421 ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg ctggataaag 
0491 aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat 
0561 tgctgctgac gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat 
0631 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg aatgcagaca 
0701 ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc 
0771 atggtccaac atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca 
0841 tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc gaacaaagag ctggcgaaag 
0911 agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc 
0981 cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc 
1051 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt actgcggtga 
1121 tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg cagactcgta tcaccaagta 
1191 atgctgtgaa atgccggatg cggcgtgaac gccttgtccg gcctacaaaa ccgaaacgta tgtaggcctg 
1261 ataagacgcg tcagcgtcgc atcaggcagt t