MG1655
W3110
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Gene Report : malF
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR12 (D)  ,  DE(xylE-malB)262  ,  OCR12-9 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  malF
Related strains
     Strains Search Search strains by gene name:  malF   Search strains by all related name:  malF b4033 ECK4025 JW3993 malJ

General information  (Go to Linear View:)
 Gene Name malF  
 Alternative name b4033,ECK4025,JW3993,malJ  
 Location, Length 4,241,554 - 4,243,098 (  -  ) ;   91.42 min ; 1545 (bp) ,   514 (aa) Go to Linear View
 Product maltose transporter subunit  
 Operon Name malEFG  
 Note part of maltose permease, periplasmic; membrane component of ABC superfamily; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols  
 Gene Ontology GO:0005215 ; transporter activity ( malF )
GO:0005351 ; sugar:hydrogen ion symporter activity ( malF )
GO:0005886 ; plasma membrane ( malF )
GO:0006810 ; transport ( malF )
GO:0008643 ; carbohydrate transport ( malF )
GO:0016020 ; membrane ( malF )
GO:0016021 ; integral to membrane ( malF )
 PID 1790465  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) MALF_ECOLI ( P02916 )
    -  Protein name Maltose transport system permease protein malF.  
    -  Synonyms  
    -  Gene name Name=malF; OrderedLocusNames=b4033;  

 Linear View (Whole Mode)
View Location
   4230.0  –  4255.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP malF (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
        PSI-BLAST  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Maltose transport system permease protein malF  
         PSI-BLAST Maltose transport system permease protein malF
    nr     (database updated : 2007.02.20 )
         BLAST maltose transporter subunit [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    BPD_TRANSP_INN_MEMBR     

Other Cross-Reference
    COG COG1175G 
    EcoCyc malF 

MMBR References
J Bacteriol. 1996;178:2255-2262
Characterization of transmembrane domains 6, 7, and 8 of MalF by mutational analysis.
Ehrle, R., C. Pick, R. Ulrich, E. Hofmann, and M. Ehrmann.
J Biol Chem. 1984;259:10896-10903
The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region.
Froschauer, S., and J. R. Beckwith.
J Biol Chem. 1991;266:20870-20876
Membrane insertion of the Escherichia coli MalF protein in cells with impaired secretion machinery.
McGovern, K., and J. Beckwith.
J Bacteriol. 1978;133(3):1358-67.
Permease-specific mutations in Salmonella typhimurium and Escherichia coli that release the glycerol, maltose, melibiose, and lactose transport systems from regulation by the phosphoenolpyruvate:sugar phosphotransferase system.
Saier MH Jr, Straud H, Massman LS, Judice JJ, Newman MJ, Feucht BU. ( 346569 )

Sequences
Amino acid
FASTA format
0001 MDVIKKKHWW QSDALKWSVL GLLGLLVGYL VVLMYAQGEY LFAITTLILS SAGLYIFANR KAYAWRYVYP 
0071 GMAGMGLFVL FPLVCTIAIA FTNYSSTNQL TFERAQEVLL DRSWQAGKTY NFGLYPAGDE WQLALSDGET 
0141 GKNYLSDAFK FGGEQKLQLK ETTAQPEGER ANLRVITQNR QALSDITAIL PDGNKVMMSS LRQFSGTQPL 
0211 YTLDGDGTLT NNQSGVKYRP NNQIGFYQSI TADGNWGDEK LSPGYTVTTG WKNFTRVFTD EGIQKPFLAI 
0281 FVWTVVFSLI TVFLTVAVGM VLACLVQWEA LRGKAVYRVL LILPYAVPSF ISILIFKGLF NQSFGEINMM 
0351 LSALFGVKPA WFSDPTTART MLIIVNTWLG YPYMMILCMG LLKAIPDDLY EASAMDGAGP FQNFFKITLP 
0421 LLIKPLTPLM IASFAFNFNN FVLIQLLTNG GPDRLGTTTP AGYTDLLVNY TYRIAFEGGG GQDFGLAAAI 
0491 ATLIFLLVGA LAIVNLKATR MKFD

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             aacgtatgta ggcctgataa gacgcgtcag 
-070 cgtcgcatca ggcagttgtt gtcggataag gcgtgaaagc cttatccgtc ctggaatgag gaagaacccc 
0001 atggatgtca ttaaaaagaa acattggtgg caaagcgacg cgctgaaatg gtcagtgcta ggtctgctcg 
0071 gcctgctggt gggttacctt gttgttttaa tgtacgcaca aggggaatac ctgttcgcca ttaccacgct 
0141 gatattgagt tcagcggggc tgtatatttt cgccaatcgt aaagcctacg cctggcgcta tgtttacccg 
0211 ggaatggctg gaatgggatt attcgtcctc ttccctctgg tctgcaccat cgccattgcc ttcaccaact 
0281 acagcagcac taaccagctg acttttgaac gtgcgcagga agtgttgtta gatcgctcct ggcaagcagg 
0351 caaaacctat aactttggtc tttacccggc gggcgatgag tggcaactgg cgctcagcga cggcgaaacc 
0421 ggcaaaaatt acctctccga cgcttttaaa tttggcggcg agcaaaaact gcaactgaaa gaaacgaccg 
0491 cccagcccga aggcgaacgc gcgaatctgc gcgtgattac ccagaatcgt caggcgctga gtgacattac 
0561 cgccattctg ccggatggca acaaagtgat gatgagctcc ctgcgccagt tttctggcac gcagccgctc 
0631 tacacactcg acggtgacgg cacgttgacg aataatcaga gcggcgtgaa atatcgtccg aataaccaaa 
0701 ttggctttta ccagtccatt accgccgacg gcaactgggg tgatgaaaag ctaagccccg gttacaccgt 
0771 gaccaccggc tggaaaaact ttacccgcgt ctttaccgac gaaggcattc agaaaccgtt cctcgccatt 
0841 ttcgtctgga ccgtggtgtt ctcgctgatc actgtctttt taacggtggc ggtcggcatg gttctggcgt 
0911 gtctggtgca gtgggaagcg ttgcgcggca aagcggtcta tcgcgtcctg ctgattctgc cctacgcggt 
0981 gccatcgttc atttcaatct tgattttcaa agggttgttt aaccagagct tcggtgaaat caacatgatg 
1051 ttgagcgcgc tgtttggcgt gaagcccgcc tggttcagcg atccgaccac cgcccgcacg atgctaatta 
1121 tcgtcaatac ctggctgggt tatccgtaca tgatgatcct ctgcatgggc ttgctgaaag cgattccgga 
1191 cgatttgtat gaagcctcag caatggatgg cgcaggtccg ttccagaact tctttaagat tacgctgccg 
1261 ctgctgatta aaccgctgac gccgctgatg atcgccagct tcgcctttaa ctttaacaac ttcgtgctga 
1331 ttcaactgtt aaccaacggc ggcccggatc gtcttggcac gaccacgcca gccggttata ccgacctgct 
1401 tgttaactac acctaccgca tcgcttttga aggcggcggg ggtcaggact tcggtctggc ggcagcaatt 
1471 gccacgctga tcttcctgct ggtgggtgcg ctggcgatag tgaacctgaa agccacgcga atgaagtttg 
1541 attaagggag ataacaaaaa tggcaatggt ccaaccgaaa tcgcaaaaag ctcgtttatt tattactcac 
1611 ctgctactgc tactttttat cgcagcgatt atgtt