|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
J Bacteriol. 1988;170(1):386-92.
Glyoxylate bypass operon of Escherichia coli: cloning and determination of the functional map.
Chung T, Klumpp DJ, LaPorte DC.
(
2826399
)
|
|
|
Deletion
|
OCR11
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
aceA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
aceA
Search strains by all related name:
aceA b4015 ECK4007 icl JW3975
|
General information (Go to Linear View: )
|
|
Gene Name
|
aceA
|
|
Alternative name
|
b4015,ECK4007,icl,JW3975
|
|
Location, Length
|
4,215,132 - 4,216,436
(
+
)
;
90.85
min
;
1305
(bp) ,
434
(aa)
|
|
Product
|
isocitrate lyase
|
|
Operon Name
|
aceBAK
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006097 - glyoxylate cycle
|
|
Function
|
enzyme; Central intermediary metabolism: Glyoxylate bypass
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( aceA )
GO:0004451
;
isocitrate lyase activity ( aceA )
GO:0005737
;
cytoplasm ( aceA )
GO:0006097
;
glyoxylate cycle ( aceA )
GO:0006099
;
tricarboxylic acid cycle ( aceA )
GO:0008152
;
metabolic process ( aceA )
GO:0016829
;
lyase activity ( aceA )
GO:0019752
;
carboxylic acid metabolic process ( aceA )
|
|
PID
|
1790445
|
EC number
(KEGG Pathway)
|
4.1.3.1
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
aceA
(
EG10022
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
ACEA_ECOLI
(
P05313
)
|
|
- Protein name
|
Isocitrate lyase
|
|
- Synonyms
|
EC 4.1.3.1, Isocitrase, Isocitratase, ICL
|
|
- Gene name
|
Name=aceA; Synonyms=icl; OrderedLocusNames=b4015, c4972;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
aceA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of The Isocitrate Lyase From The A219c Mutant Of Escherichia Coli
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Isocitrate lyase (Isocitrase) (Isocitratase) (ICL)
|
|
PSI-BLAST
|
Isocitrate lyase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
isocitrate lyase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
RNP_1
ISOCITRATE_LYASE
|
|
MMBR References
|
J Bacteriol. 1982;149(1):173-80.
Genetic regulation of the glyoxylate shunt in Escherichia coli K-12.
Maloy SR, Nunn WD.
(
7033207
)
|
J Bacteriol. 1988;170(10):4528-36.
Isolation, hyperexpression, and sequencing of the aceA gene encoding isocitrate lyase in Escherichia coli.
Matsuoka M, McFadden BA.
(
3049537
)
|
Nucleic Acids Res. 1988;16(12):5689
Nucleotide sequence of the aceA gene coding for isocitrate lyase in Escherichia coli.
Rieul C, Bleicher F, Duclos B, Cortay JC, Cozzone AJ.
(
3290857
)
|
J Bacteriol. 1993;175:4572-4575
Relative expression of the products of the glyoxylate bypass operon: contributions of transcription and translation.
Chung, T., E. Resnik, C. S. Stueland, and D. C. LaPorte.
|
Biochimie. 1989;71(9-10):1043-9.
Utilization of acetate in Escherichia coli: structural organization and differential expression of the ace operon.
Cortay JC, Bleicher F, Duclos B, Cenatiempo Y, Gautier C, Prato JL, Cozzone AJ.
(
2512996
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKTRTQQIEE LQKEWTQPRW EGITRPYSAE DVVKLRGSVN PECTLAQLGA AKMWRLLHGE SKKGYINSLG
0071 ALTGGQALQQ AKAGIEAVYL SGWQVAADAN LAASMYPDQS LYPANSVPAV VERINNTFRR ADQIQWSAGI
0141 EPGDPRYVDY FLPIVADAEA GFGGVLNAFE LMKAMIEAGA AAVHFEDQLA SVKKCGHMGG KVLVPTQEAI
0211 QKLVAARLAA DVTGVPTLLV ARTDADAADL ITSDCDPYDS EFITGERTSE GFFRTHAGIE QAISRGLAYA
0281 PYADLVWCET STPDLELARR FAQAIHAKYP GKLLAYNCSP SFNWQKNLDD KTIASFQQQL SDMGYKFQFI
0351 TLAGIHSMWF NMFDLANAYA QGEGMKHYVE KVQQPEFAAA KDGYTFVSHQ QEVGTGYFDK VTTIIQGGTS
0421 SVTALTGSTE ESQF
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TGGAACAGAT CACCACTTCC GATGAGTTAA
-070 TTGATTTCCT GACCCTGCCA GGCTACCGCC TGTTAGCGTA AACCACCACA TAACTATGGA GCATCTGCAC
0001 atgaaaaccc gtacacaaca aattgaagaa ttacagaaag agtggactca accgcgttgg gaaggcatta
0071 ctcgcccata cagtgcggaa gatgtggtga aattacgcgg ttcagtcaat cctgaatgca cgctggcgca
0141 actgggcgca gcgaaaatgt ggcgtctgct gcacggtgag tcgaaaaaag gctacatcaa cagcctcggc
0211 gcactgactg gcggtcaggc gctgcaacag gcgaaagcgg gtattgaagc agtctatctg tcgggatggc
0281 aggtagcggc ggacgctaac ctggcggcca gcatgtatcc ggatcagtcg ctctatccgg caaactcggt
0351 gccagctgtg gtggagcgga tcaacaacac cttccgtcgt gccgatcaga tccaatggtc cgcgggcatt
0421 gagccgggcg atccgcgcta tgtcgattac ttcctgccga tcgttgccga tgcggaagcc ggttttggcg
0491 gtgtcctgaa tgcctttgaa ctgatgaaag cgatgattga agccggtgca gcggcagttc acttcgaaga
0561 tcagctggcg tcagtgaaga aatgcggtca catgggcggc aaagttttag tgccaactca ggaagctatt
0631 cagaaactgg tcgcggcgcg tctggcagct gacgtgacgg gcgttccaac cctgctggtt gcccgtaccg
0701 atgctgatgc ggcggatctg atcacctccg attgcgaccc gtatgacagc gaatttatta ccggcgagcg
0771 taccagtgaa ggcttcttcc gtactcatgc gggcattgag caagcgatca gccgtggcct ggcgtatgcg
0841 ccatatgctg acctggtctg gtgtgaaacc tccacgccgg atctggaact ggcgcgtcgc tttgcacaag
0911 ctatccacgc gaaatatccg ggcaaactgc tggcttataa ctgctcgccg tcgttcaact ggcagaaaaa
0981 cctcgacgac aaaactattg ccagcttcca gcagcagctg tcggatatgg gctacaagtt ccagttcatc
1051 accctggcag gtatccacag catgtggttc aacatgtttg acctggcaaa cgcctatgcc cagggcgagg
1121 gtatgaagca ctacgttgag aaagtgcagc agccggaatt tgccgccgcg aaagatggct ataccttcgt
1191 atctcaccag caggaagtgg gtacaggtta cttcgataaa gtgacgacta ttattcaggg cggcacgtct
1261 tcagtcaccg cgctgaccgg ctccactgaa gaatcgcagt tctaaGCAAC AACAACCGTT GCTGACTGTA
1331 GGCCGGATAA GGCGTTCACG CCGCATCCGG CAATCGGTGC ACGATGCCTG ATGCGACGCT TGCGCGTCTT
1401 ATCAT
|
