MG1655
W3110
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Gene Report : purH
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR11 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  purH
Related strains
     Strains Search Search strains by gene name:  purH   Search strains by all related name:  purH b4006 ECK3998 JW3970

General information  (Go to Linear View:)
 Gene Name purH  
 Alternative name b4006,ECK3998,JW3970  
 Location, Length 4,203,966 - 4,205,555 (  -  ) ;   90.61 min ; 1590 (bp) ,   529 (aa) Go to Linear View
 Product fused IMPcyclohydrolase/phosphoribosylaminoimidazolecarboxamide formyltransferase  
 Operon Name purHD  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006164 - purine nucleotide biosynthetic process; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion  
 Function enzyme; Purine ribonucleotide biosynthesis  
 Gene Ontology GO:0003824 ; catalytic activity ( purH )
GO:0003937 ; IMP cyclohydrolase activity ( purH )
GO:0004643 ; phosphoribosylaminoimidazolecarboxamide formyltransferase activity ( purH )
GO:0005737 ; cytoplasm ( purH )
GO:0006164 ; purine nucleotide biosynthetic process ( purH )
GO:0006188 ; IMP biosynthetic process ( purH )
GO:0016740 ; transferase activity ( purH )
GO:0016787 ; hydrolase activity ( purH )
 PID 1790439  
 EC number
  (KEGG Pathway)
 
2.1.2.3   3.5.4.10  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene purH ( EG10795 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PUR9_ECOLI ( P15639 )
    -  Protein name Bifunctional purine biosynthesis protein purH  
    -  Synonyms  
    -  Gene name Name=purH; OrderedLocusNames=b4006;  

 Linear View (Whole Mode)
View Location
   4195.0  –  4220.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP purH (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Human Atic In Complex With Xmp  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Bifunctional purine biosynthesis protein purH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase  
         PSI-BLAST Bifunctional purine biosynthesis protein purH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase
    nr     (database updated : 2007.02.20 )
         BLAST bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION     

Other Cross-Reference
    EcoCyc purH 

MMBR References
Mol Microbiol. 1990;4(3):381-92.
Purine biosynthesis in Escherichia coli K12: structure and DNA sequence studies of the purHD locus.
Flannigan KA, Hennigan SH, Vogelbacker HH, Gots JS, Smith JM. ( 2192230 )
Mol Gen Genet. 1989;216(2-3):475-83.
The effect of DnaA protein levels and the rate of initiation at oriC on transcription originating in the ftsQ and ftsA genes: in vivo experiments.
Masters M, Paterson T, Popplewell AG, Owen-Hughes T, Pringle JH, Begg KJ. ( 2546042 )
J Biol Chem. 1989;264:21239-21246
Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli.
Aiba, H., and K. Mizobuchi.
J Bacteriol. 1991;173(11):3274-5.
Fine mapping of the rrnE, purHD, and hydGH operons on the Escherichia coli chromosome.
Peterson JW, Chopra AK, Prasad R. ( 2045358 )

Sequences
Amino acid
FASTA format
0001 MQQRRPVRRA LLSVSDKAGI VEFAQALSAR GVELLSTGGT ARLLAEKGLP VTEVSDYTGF PEMMDGRVKT 
0071 LHPKVHGGIL GRRGQDDAIM EEHQIQPIDM VVVNLYPFAQ TVAREGCSLE DAVENIDIGG PTMVRSAAKN 
0141 HKDVAIVVKS SDYDAIIKEM DDNEGSLTLA TRFDLAIKAF EHTAAYDSMI ANYFGSMVPA YHGESKEAAG 
0211 RFPRTLNLNF IKKLDMRYGE NSHQQAAFYI EENVKEASVA TATQVQGKAL SYNNIADTDA ALECVKEFAE 
0281 PACVIVKHAN PCGVAIGNSI LDAYDRAYKT DPTSAFGGII AFNRELDAET AQAIISRQFV EVIIAPSASE 
0351 EALKITAAKQ NVRVLTCGQW GERVPGLDFK RVNGGLLVQD RDLGMVGAEE LRVVTKRQPS EQELRDALFC 
0421 WKVAKFVKSN AIVYAKNNMT IGIGAGQMSR VYSAKIAGIK AADEGLEVKG SSMASDAFFP FRDGIDAAAA 
0491 AGVTCVIQPG GSIRDDEVIA AADEHGIAML FTDMRHFRH

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             cgggcgaaaa ataaggatgc cccgttaggg 
-070 gcgttagctg agtttttcgc gaaaaattca gctaacgctc tctgtaatag tcaaatccag gggatttacc 
0001 atgcaacaac gtcgtccagt ccgccgcgct ctgctcagtg tttctgacaa agccggtatc gtcgaattcg 
0071 cccaggcact ttccgcacgc ggtgtggagc tgctgtctac agggggcact gcccgtctgt tagcagaaaa 
0141 aggtctgccg gtaaccgaag tttccgatta caccggtttc ccggagatga tggatggacg cgtgaagacc 
0211 ctgcatccga aagtacatgg tggcattctg ggccgtcgcg gccaggacga tgccattatg gaagaacatc 
0281 agatccagcc tatcgatatg gtggttgtta acctgtatcc gttcgcccag accgtggccc gtgaaggttg 
0351 ctcgctggaa gatgcggttg agaacatcga tatcggcggc ccaacgatgg tgcgctccgc cgccaagaac 
0421 cataaagatg tcgcaatcgt ggtgaagagc agcgactatg acgccattat taaagagatg gatgacaacg 
0491 aaggatcgct gacgcttgca acccgtttcg acctcgccat caaagccttc gaacacactg ccgcctacga 
0561 cagcatgatt gccaactact tcggcagcat ggttccggct taccacggtg aaagcaaaga agccgccggt 
0631 cgcttcccac gcacgctgaa cctgaacttc attaagaagc tggatatgcg ttacggcgag aacagccacc 
0701 agcaggctgc cttctatata gaagagaatg tgaaagaagc ctccgttgct accgcaaccc aggttcaggg 
0771 taaagccctc tcttataaca acatcgccga taccgatgcg gcgctggagt gcgtgaaaga gttcgccgag 
0841 ccggcatgtg tgattgtgaa gcacgccaac ccttgcggcg tggctatcgg caattccatt cttgatgctt 
0911 acgatcgcgc gtacaaaacc gacccaacct ccgcattcgg cggcatcatt gcctttaacc gcgagctgga 
0981 tgcggaaacc gcacaggcca tcatttctcg tcagtttgtc gaagtgatta ttgcgccgtc cgccagcgaa 
1051 gaagccctga aaatcaccgc cgccaaacag aacgtacgcg ttctgacctg cggtcagtgg ggcgagcgtg 
1121 ttccgggcct cgatttcaaa cgcgtgaacg gcggtctgct ggttcaggat cgtgacctgg ggatggtcgg 
1191 tgcggaagaa ctgcgcgtgg tgaccaaacg tcagccgagc gaacaggaac tgcgtgatgc gctgttctgc 
1261 tggaaggtgg cgaagtttgt gaaatccaac gctatcgtct atgccaaaaa caatatgact atcggcattg 
1331 gcgcgggcca gatgagccgc gtgtactccg caaaaatcgc cggtattaaa gcggccgatg aaggcctgga 
1401 agtgaaaggt tcctcgatgg cttctgacgc gttcttcccg ttccgcgacg gtattgatgc cgccgccgct 
1471 gcgggcgtga cctgcgtaat ccagcctggc ggttctatcc gtgatgacga agtgattgcc gccgccgacg 
1541 agcacggtat tgcgatgctc ttcaccgaca tgcgccactt ccgccattaa tggagcaata gatgaaagta 
1611 ttagtgattg gtaacggcgg gcgcgagcac gcgctggcct ggaaagcggc ccagtcgccg ctggttgaga 
1681 ctgtttttgt