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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCR11
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
purD
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Related strains
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Strains Search
|
Search strains by gene name:
purD
Search strains by all related name:
purD adth b4005 ECK3997 JW3969
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General information (Go to Linear View: )
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Gene Name
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purD
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Alternative name
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adth,b4005,ECK3997,JW3969
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Location, Length
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4,202,665 - 4,203,954
(
-
)
;
90.58
min
;
1290
(bp) ,
429
(aa)
|
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Product
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phosphoribosylglycinamide synthetase phosphoribosylamine-glycine ligase
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Operon Name
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purHD
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Note
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phosphoribosylglycinamide synthetase = GAR synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006164 - purine nucleotide biosynthetic process
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Function
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enzyme; Purine ribonucleotide biosynthesis
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Gene Ontology
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GO:0003824
;
catalytic activity ( purD )
GO:0004637
;
phosphoribosylamine-glycine ligase activity ( purD )
GO:0005524
;
ATP binding ( purD )
GO:0006164
;
purine nucleotide biosynthetic process ( purD )
GO:0009113
;
purine base biosynthetic process ( purD )
GO:0016874
;
ligase activity ( purD )
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PID
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1790438
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EC number
(KEGG Pathway)
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6.3.4.13
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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PUR2_ECOLI
(
P15640
)
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- Protein name
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Phosphoribosylamine--glycine ligase
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- Synonyms
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EC 6.3.4.13, GARS, Glycinamide ribonucleotide synthetase, Phosphoribosylglycinamide synthetase
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- Gene name
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Name=purD; OrderedLocusNames=b4005;
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MMBR References
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Mol Microbiol. 1990;4(3):381-92.
Purine biosynthesis in Escherichia coli K12: structure and DNA sequence studies of the purHD locus.
Flannigan KA, Hennigan SH, Vogelbacker HH, Gots JS, Smith JM.
(
2192230
)
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Mol Gen Genet. 1989;216(2-3):475-83.
The effect of DnaA protein levels and the rate of initiation at oriC on transcription originating in the ftsQ and ftsA genes: in vivo experiments.
Masters M, Paterson T, Popplewell AG, Owen-Hughes T, Pringle JH, Begg KJ.
(
2546042
)
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J Biol Chem. 1989;264:21239-21246
Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli.
Aiba, H., and K. Mizobuchi.
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Biochemistry. 1990;29(1):218-27.
Glycinamide ribonucleotide synthetase from Escherichia coli: cloning, overproduction, sequencing, isolation, and characterization.
Cheng YS, Shen Y, Rudolph J, Stern M, Stubbe J, Flannigan KA, Smith JM.
(
2182115
)
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| Sequences |
Amino acid
FASTA format
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0001 MKVLVIGNGG REHALAWKAA QSPLVETVFV APGNAGTALE PALQNVAIGV TDIPALLDFA QNEKIDLTIV
0071 GPEAPLVKGV VDTFRAAGLK IFGPTAGAAQ LEGSKAFTKD FLARHKIPTA EYQNFTEVEP ALAYLREKGA
0141 PIVIKADGLA AGKGVIVAMT LEEAEAAVHD MLAGNAFGDA GHRIVIEEFL DGEEASFIVM VDGEHVLPMA
0211 TSQDHKRVGD KDTGPNTGGM GAYSPAPVVT DDVHQRTMER IIWPTVKGMA AEGNTYTGFL YAGLMIDKQG
0281 NPKVIEFNCR FGDPETQPIM LRMKSDLVEL CLAACESKLD EKTSEWDERA SLGVVMAAGG YPGDYRTGDV
0351 IHGLPLEEVA GGKVFHAGTK LADDEQVVTN GGRVLCVTAL GHTVAEAQKR AYALMTDIHW DDCFCRKDIG
0421 WRAIEREQN
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 gttctatccg tgatgacgaa gtgattgccg
-070 ccgccgacga gcacggtatt gcgatgctct tcaccgacat gcgccacttc cgccattaat ggagcaatag
0001 atgaaagtat tagtgattgg taacggcggg cgcgagcacg cgctggcctg gaaagcggcc cagtcgccgc
0071 tggttgagac tgtttttgtt gctccgggta atgcaggcac tgcactggaa cccgcgctgc aaaacgttgc
0141 tattggcgtg accgatatcc cggcgctgtt ggatttcgca caaaacgaaa agattgatct gaccatcgtc
0211 ggcccggaag cgccgctggt gaaaggcgtg gtcgatacct tccgcgccgc cgggctgaaa atcttcggcc
0281 caaccgcagg tgcggcccaa ctggaaggct caaaagcgtt taccaaagat ttcctggccc gccataagat
0351 ccctacggcg gaataccaga acttcaccga ggtagaacct gcgctggcgt atctgcgtga gaaaggcgcg
0421 ccaatcgtca ttaaagcgga cggtctggct gccgggaaag gcgttatcgt ggcgatgacg ctggaagaag
0491 cggaagcggc tgttcacgat atgctggcgg gcaacgcttt tggcgacgcg ggtcatcgca tcgttatcga
0561 agagttcctc gatggcgaag aagcgagctt tatcgtgatg gtggacggcg agcatgtgct gccgatggct
0631 accagccagg atcacaaacg cgtaggcgat aaagataccg gaccaaacac cggcgggatg ggcgcttact
0701 cccccgcgcc ggtagtaacc gatgacgttc atcagcgcac catggaacgt attatctggc caaccgtgaa
0771 aggcatggcg gcggaaggca acacctacac cggttttctc tacgcgggcc tgatgatcga caaacagggc
0841 aatccgaagg ttattgaatt taactgccgc tttggcgatc cggaaaccca gccgattatg ctgcgcatga
0911 agtccgatct ggttgagctc tgcctggcgg cctgtgaaag caaactggac gagaaaacgt ccgagtggga
0981 tgaacgcgct tctctcggcg tggtgatggc tgcgggtgga tatccgggtg attaccgcac cggtgacgtg
1051 atccacggcc tgccgctgga agaagtggca ggcggcaaag tgttccacgc gggcacaaaa ctggcggatg
1121 acgagcaggt agtgaccaac ggcgggcgcg tactgtgcgt caccgcgctg ggtcataccg tggcagaagc
1191 gcagaaacgc gcctatgcct taatgaccga tatccactgg gacgactgct tctgccggaa agatatcggc
1261 tggcgcgcta tcgaacgcga gcagaactaa cgcgacagtt ttgccaatag cgttttgcgc gtgatcccta
1331 actgacgggc ggcttcggtt ttgttgccgc ccgttttctc cagcgccgcc agaatcacct
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