MG1655
W3110
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Gene Report : purD
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR11 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  purD
Related strains
     Strains Search Search strains by gene name:  purD   Search strains by all related name:  purD adth b4005 ECK3997 JW3969

General information  (Go to Linear View:)
 Gene Name purD  
 Alternative name adth,b4005,ECK3997,JW3969  
 Location, Length 4,202,665 - 4,203,954 (  -  ) ;   90.58 min ; 1290 (bp) ,   429 (aa) Go to Linear View
 Product phosphoribosylglycinamide synthetase phosphoribosylamine-glycine ligase  
 Operon Name purHD  
 Note phosphoribosylglycinamide synthetase = GAR synthetase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006164 - purine nucleotide biosynthetic process  
 Function enzyme; Purine ribonucleotide biosynthesis  
 Gene Ontology GO:0003824 ; catalytic activity ( purD )
GO:0004637 ; phosphoribosylamine-glycine ligase activity ( purD )
GO:0005524 ; ATP binding ( purD )
GO:0006164 ; purine nucleotide biosynthetic process ( purD )
GO:0009113 ; purine base biosynthetic process ( purD )
GO:0016874 ; ligase activity ( purD )
 PID 1790438  
 EC number
  (KEGG Pathway)
 
6.3.4.13  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PUR2_ECOLI ( P15640 )
    -  Protein name Phosphoribosylamine--glycine ligase  
    -  Synonyms EC 6.3.4.13, GARS, Glycinamide ribonucleotide synthetase, Phosphoribosylglycinamide synthetase  
    -  Gene name Name=purD; OrderedLocusNames=b4005;  

 Linear View (Whole Mode)
View Location
   4190.0  –  4215.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP purD (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Glycinamide Ribonucleotide Synthetase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Phosphoribosylamine--glycine ligase  
         PSI-BLAST Phosphoribosylamine--glycine ligase
    nr     (database updated : 2007.02.20 )
         BLAST phosphoribosylamine--glycine ligase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    GARS     

Other Cross-Reference
    COG COG0151F 
    EcoCyc purD 

MMBR References
Mol Microbiol. 1990;4(3):381-92.
Purine biosynthesis in Escherichia coli K12: structure and DNA sequence studies of the purHD locus.
Flannigan KA, Hennigan SH, Vogelbacker HH, Gots JS, Smith JM. ( 2192230 )
Mol Gen Genet. 1989;216(2-3):475-83.
The effect of DnaA protein levels and the rate of initiation at oriC on transcription originating in the ftsQ and ftsA genes: in vivo experiments.
Masters M, Paterson T, Popplewell AG, Owen-Hughes T, Pringle JH, Begg KJ. ( 2546042 )
J Biol Chem. 1989;264:21239-21246
Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli.
Aiba, H., and K. Mizobuchi.
Biochemistry. 1990;29(1):218-27.
Glycinamide ribonucleotide synthetase from Escherichia coli: cloning, overproduction, sequencing, isolation, and characterization.
Cheng YS, Shen Y, Rudolph J, Stern M, Stubbe J, Flannigan KA, Smith JM. ( 2182115 )

Sequences
Amino acid
FASTA format
0001 MKVLVIGNGG REHALAWKAA QSPLVETVFV APGNAGTALE PALQNVAIGV TDIPALLDFA QNEKIDLTIV 
0071 GPEAPLVKGV VDTFRAAGLK IFGPTAGAAQ LEGSKAFTKD FLARHKIPTA EYQNFTEVEP ALAYLREKGA 
0141 PIVIKADGLA AGKGVIVAMT LEEAEAAVHD MLAGNAFGDA GHRIVIEEFL DGEEASFIVM VDGEHVLPMA 
0211 TSQDHKRVGD KDTGPNTGGM GAYSPAPVVT DDVHQRTMER IIWPTVKGMA AEGNTYTGFL YAGLMIDKQG 
0281 NPKVIEFNCR FGDPETQPIM LRMKSDLVEL CLAACESKLD EKTSEWDERA SLGVVMAAGG YPGDYRTGDV 
0351 IHGLPLEEVA GGKVFHAGTK LADDEQVVTN GGRVLCVTAL GHTVAEAQKR AYALMTDIHW DDCFCRKDIG 
0421 WRAIEREQN

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gttctatccg tgatgacgaa gtgattgccg 
-070 ccgccgacga gcacggtatt gcgatgctct tcaccgacat gcgccacttc cgccattaat ggagcaatag 
0001 atgaaagtat tagtgattgg taacggcggg cgcgagcacg cgctggcctg gaaagcggcc cagtcgccgc 
0071 tggttgagac tgtttttgtt gctccgggta atgcaggcac tgcactggaa cccgcgctgc aaaacgttgc 
0141 tattggcgtg accgatatcc cggcgctgtt ggatttcgca caaaacgaaa agattgatct gaccatcgtc 
0211 ggcccggaag cgccgctggt gaaaggcgtg gtcgatacct tccgcgccgc cgggctgaaa atcttcggcc 
0281 caaccgcagg tgcggcccaa ctggaaggct caaaagcgtt taccaaagat ttcctggccc gccataagat 
0351 ccctacggcg gaataccaga acttcaccga ggtagaacct gcgctggcgt atctgcgtga gaaaggcgcg 
0421 ccaatcgtca ttaaagcgga cggtctggct gccgggaaag gcgttatcgt ggcgatgacg ctggaagaag 
0491 cggaagcggc tgttcacgat atgctggcgg gcaacgcttt tggcgacgcg ggtcatcgca tcgttatcga 
0561 agagttcctc gatggcgaag aagcgagctt tatcgtgatg gtggacggcg agcatgtgct gccgatggct 
0631 accagccagg atcacaaacg cgtaggcgat aaagataccg gaccaaacac cggcgggatg ggcgcttact 
0701 cccccgcgcc ggtagtaacc gatgacgttc atcagcgcac catggaacgt attatctggc caaccgtgaa 
0771 aggcatggcg gcggaaggca acacctacac cggttttctc tacgcgggcc tgatgatcga caaacagggc 
0841 aatccgaagg ttattgaatt taactgccgc tttggcgatc cggaaaccca gccgattatg ctgcgcatga 
0911 agtccgatct ggttgagctc tgcctggcgg cctgtgaaag caaactggac gagaaaacgt ccgagtggga 
0981 tgaacgcgct tctctcggcg tggtgatggc tgcgggtgga tatccgggtg attaccgcac cggtgacgtg 
1051 atccacggcc tgccgctgga agaagtggca ggcggcaaag tgttccacgc gggcacaaaa ctggcggatg 
1121 acgagcaggt agtgaccaac ggcgggcgcg tactgtgcgt caccgcgctg ggtcataccg tggcagaagc 
1191 gcagaaacgc gcctatgcct taatgaccga tatccactgg gacgactgct tctgccggaa agatatcggc 
1261 tggcgcgcta tcgaacgcga gcagaactaa cgcgacagtt ttgccaatag cgttttgcgc gtgatcccta 
1331 actgacgggc ggcttcggtt ttgttgccgc ccgttttctc cagcgccgcc agaatcacct