|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
OCR10,11-16 (BK5-16 / AK7-16)
(D)
,
OCR11
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
thiF
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
thiF
Search strains by all related name:
thiF b3992 ECK3984 f245 JW3956 thiA
|
General information (Go to Linear View: )
|
|
Gene Name
|
thiF
|
|
Alternative name
|
b3992,ECK3984,f245,JW3956,thiA
|
|
Location, Length
|
4,190,844 - 4,191,599
(
-
)
;
90.33
min
;
756
(bp) ,
251
(aa)
|
|
Product
|
adenylyltransferase, modifies ThiS C-terminus
|
|
Operon Name
|
thiCEFSGH
|
|
Note
|
adenylyltransferase; GO_process: GO:0009228 - thiamin biosynthetic process
|
|
Function
|
enzyme; Biosynthesis of cofactors, carriers: Thiamin
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( thiF )
GO:0005488
;
binding ( thiF )
GO:0008152
;
metabolic process ( thiF )
GO:0009228
;
thiamin biosynthetic process ( thiF )
GO:0016740
;
transferase activity ( thiF )
GO:0016779
;
nucleotidyltransferase activity ( thiF )
|
|
PID
|
87082356
|
EC number
(KEGG Pathway)
|
2.7.7.-
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
THIF_ECOLI
(
P30138
;
P76780
)
|
|
- Protein name
|
Adenylyltransferase thiF
|
|
- Synonyms
|
EC 2.7.7.-
|
|
- Gene name
|
Name=thiF; OrderedLocusNames=b3992;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
thiF
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain B, Structure Of The Covalent Acyl-Adenylate Form Of The Moeb- Moad Protein Complex
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Adenylyltransferase thiF
|
|
PSI-BLAST
|
Molybdopterin biosynthesis protein moeB
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
Chain A, Structural Analysis Of Escherichia Coli Thif
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
CAMP_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
J Bacteriol. 1993;175:982-992
Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli.
Vander Horn, P. B., A. D. Backstrom, V. J. Stewart, and T. P. Begley.
|
| Sequences |
Amino acid
FASTA format
|
0001 MNDRDFMRYS RQILLDDIAL DGQQKLLDSQ VLIIGLGGLG TPAALYLAGA GVGTLVLADD DDVHLSNLQR
0071 QILFTTEDID RPKSQVSQQR LTQLNPDIQL TALQQRLTGE ALKDAVARAD VVLDCTDNMA TRQEINAACV
0141 ALNTPLITAS AVGFGGQLMV LTPPWEQGCY RCLWPDNQEP ERNCRTAGVV GPVVGVMGTL QALEAIKLLS
0211 GIETPAGELR LFDGKSSQWR SLALRRASGC PVCGGSNADP V
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gcaacgggtg tcggcagtat cgccgtcgtc
-070 agcgccatta ctcaagccgc agactggcgt ttggcaacgg cacagttgct ggaaattgca ggagttggcg
0001 atgaatgacc gtgactttat gcgttatagc cgccaaatcc tgctcgacga tatcgctctg gacgggcagc
0071 aaaaactgct cgacagccag gtgctgatta tcggtctggg cgggctgggt acacctgctg cgctgtacct
0141 ggcgggcgct ggcgtcggga cgctggtact ggcagatgac gacgatgtgc atttaagcaa tctgcaacga
0211 caaatcctct ttaccactga agatatcgat cgcccgaaat cgcaggtcag ccaacagcga ctgacacagt
0281 tgaatcccga cattcaactg acagcattac aacaacggtt aacgggtgag gcgttaaaag atgcggttgc
0351 acgggccgat gtggtgctcg actgtaccga caatatggcg actcgccagg agattaatgc cgcctgcgtg
0421 gcactcaaca cgccgcttat caccgccagc gcggtcggat ttggcggtca gttgatggta ctgacgccgc
0491 cctgggagca ggggtgttac cgctgcctgt ggccagataa ccaggagcca gaacgcaact gccgcacggc
0561 gggcgtggtt ggcccggtgg tcggggttat gggcactttg caggcactgg aagccattaa gttattaagc
0631 ggtatagaga cacctgcggg agaactccga ctgttcgacg gtaaatcgag ccagtggcgc agcctggcgt
0701 tgcgccgcgc cagtggttgc ccggtatgcg gaggaagcaa tgcagatcct gtttaacgat caagcgatgc
0771 agtgcgccgc cgggcaaact gttcacgaac tactggagca actcgaccaa cgacaagcgg gcgcggctct
0841 ggcgattaat cagcaa
|
