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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1982;152(2):736-46.
An amber mutation in the gene encoding the beta' subunit of Escherichia coli RNA polymerase.
Ridley SP, Oeschger MP.
(
6290449
)
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Deletion
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OCR10,11-11 (AK1-16 / BK2-16)
(D)
,
OCR10,11-16 (BK5-16 / AK7-16)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
rpoC
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Related strains
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Strains Search
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Search strains by gene name:
rpoC
Search strains by all related name:
rpoC b3988 ECK3979 JW3951 o1407 tabB tabD
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General information (Go to Linear View: )
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Gene Name
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rpoC
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Alternative name
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b3988,ECK3979,JW3951,o1407,tabB,tabD
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Location, Length
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4,183,373 - 4,187,596
(
+
)
;
90.17
min
;
4224
(bp) ,
1407
(aa)
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Product
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RNA polymerase, beta prime subunit
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Operon Name
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rplKAJL-rpoBC
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006350 - transcription
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Function
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enzyme; RNA synthesis, modification, DNA transcription
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Gene Ontology
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GO:0003677
;
DNA binding ( rpoC )
GO:0003899
;
DNA-directed RNA polymerase activity ( rpoC )
GO:0005515
;
protein binding ( rpoC )
GO:0005737
;
cytoplasm ( rpoC )
GO:0006350
;
transcription ( rpoC )
GO:0006351
;
transcription, DNA-dependent ( rpoC )
GO:0016740
;
transferase activity ( rpoC )
GO:0016779
;
nucleotidyltransferase activity ( rpoC )
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PID
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2367335
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EC number
(KEGG Pathway)
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2.7.7.6
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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rpoC
(
EG10895
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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RPOC_ECOLI
(
P00577
;
P00578; P78134
)
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- Protein name
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DNA-directed RNA polymerase beta' chain
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- Synonyms
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EC 2.7.7.6, RNAP beta' subunit, Transcriptase beta' chain, RNA polymerase beta' subunit
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- Gene name
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OrderedLocusNames=b3988, z5561, ECs4911, SF4061, S3674;
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MMBR References
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J Mol Biol. 1987;194(4):609-20.
Transcription products from the rplKAJL-rpoBC gene cluster.
Downing WL, Dennis PP.
(
2443706
)
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Mol Microbiol. 1996;19(3):483-93.
Synthesis of the beta and beta' subunits of Escherichia coli RNA polymerase is autogenously regulated in vivo by both transcriptional and translational mechanisms.
Dykxhoorn DM, St Pierre R, Linn T.
(
8830239
)
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Proc Natl Acad Sci U S A. 1980;77:2124-2128
Bacteriophage vehicle for the direct cloning of Escherichia coli promoter DNA sequences: feedback regulation of the rplJL-rpoBC operon.
Holowachuk, E. W., J. D. Friesen, and N. Fiil.
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Mol Gen Genet. 1989;216(2-3):269-75.
Three rpoBC mutations that suppress the termination defects of rho mutants also affect the functions of nusA mutants.
Jin DJ, Gross CA.
(
2664452
)
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Mol Gen Genet. 1980;179(3):489-96.
Autogenous and post-transcriptional regulation of Escherichia coli RNA polymerase synthesis in vitro.
Kajitani M, Fukuda R, Ishihama A.
(
6160369
)
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Proc Natl Acad Sci U S A. 1980;77:3331-3335
Attenuation and processing of RNA from the rplJL-rpoBC transcription unit of Escherichia coli.
Barry, G., C. Squires, and C. L. Squires.
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Proc Natl Acad Sci U S A. 1979;76:4922-4926
Control features within the rplJL-rpoBC transcription unit of Escherichia coli.
Barry, G., C. L. Squires, and C. Squires.
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J Bacteriol. 1979;140(2):479-89.
Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome.
Linn T, Goman M, Scaife J.
(
159290
)
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J Bacteriol. 1981;147(1):25-35.
Transcriptional and post-transcriptional control of ribosomal protein and ribonucleic acid polymerase genes.
Little R, Fiil NP, Dennis PP.
(
7016843
)
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Mol Gen Genet. 1981;184(3):548-50.
Internal promoters of the rpoBC operon of Escherichia coli.
Ma JC, Newman AJ, Hayward RS.
(
6278264
)
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Mol Gen Genet. 1980;177(3):527-33.
Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12.
Newman A, Hayward RS.
(
6246404
)
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Nucleic Acids Res. 1982;10:4035-4044
The primary structure of E. coli RNA polymerase. Nucleotide sequence of the rpoC gene and amino acid sequence of the B'-subunit.
Ovchinnikov, Y. A., G. S. Monastyrskaya, V. V. Gubanov, S. O. Guryev, I. S. Salomatina, T. M. Shuvaeva, V. M. Lipkin, and E. D. Sverdlov.
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Eur J Biochem. 1982;116:621-629
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the B-subunit.
Ovchinnikov, Y. A., G. S. Monastyrskaya, V. V. Gubanov, S. O. Guryev, O. Y. Chertov, N. N. Modyanov, V. A. Grinkevich, I. A. Makarova, T. V. Marchenko, I. N. Polovnikova, V. M. Lipkin, and E. D. Sverdlov.
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J Bacteriol. 1984;158(1):279-85.
Relative activities of the transcriptional regulatory sites in the rplKAJLrpoBC gene cluster of Escherichia coli.
Ralling G, Linn T.
(
6325390
)
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Nucleic Acids Res. 1981;9:6827-6840
Nucleotide sequence at the end of the gene for the RNA polymerase B' subunit (rpoC).
Squires, C., A. Krainer, G. Barry, W.-F. Shen, and C. L. Squires.
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J Mol Biol. 1991;218(1):23-31.
In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein-RNA polymerase gene cluster of Escherichia coli.
Steward KL, Linn T.
(
1825852
)
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Mol Gen Genet. 1981;183(2):277-82.
In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli.
Bruckner R, Matzura H.
(
7035827
)
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J Bacteriol. 1980;144(3):904-16.
Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced internal promoter mutant.
An G, Friesen JD.
(
7002914
)
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| Sequences |
Amino acid
FASTA format
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0001 MKDLLKFLKA QTKTEEFDAI KIALASPDMI RSWSFGEVKK PETINYRTFK PERDGLFCAR IFGPVKDYEC
0071 LCGKYKRLKH RGVICEKCGV EVTQTKVRRE RMGHIELASP TAHIWFLKSL PSRIGLLLDM PLRDIERVLY
0141 FESYVVIEGG MTNLERQQIL TEEQYLDALE EFGDEFDAKM GAEAIQALLK SMDLEQECEQ LREELNETNS
0211 ETKRKKLTKR IKLLEAFVQS GNKPEWMILT VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLK
0281 RLLDLAAPDI IVRNEKRMLQ EAVDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS
0351 GRSVITVGPY LRLHQCGLPK KMALELFKPF IYGKLELRGL ATTIKAAKKM VEREEAVVWD ILDEVIREHP
0421 VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH PLVCAAYNAD FDGDQMAVHV PLTLEAQLEA RALMMSTNNI
0491 LSPANGEPII VPSQDVVLGL YYMTRDCVNA KGEGMVLTGP KEAERLYRSG LASLHARVKV RITEYEKDAN
0561 GELVAKTSLK DTTVGRAILW MIVPKGLPYS IVNQALGKKA ISKMLNTCYR ILGLKPTVIF ADQIMYTGFA
0631 YAARSGASVG IDDMVIPEKK HEIISEAEAE VAEIQEQFQS GLVTAGERYN KVIDIWAAAN DRVSKAMMDN
0701 LQTETVINRD GQEEKQVSFN SIYMMADSGA RGSAAQIRQL AGMRGLMAKP DGSIIETPIT ANFREGLNVL
0771 QYFISTHGAR KGLADTALKT ANSGYLTRRL VDVAQDLVVT EDDCGTHEGI MMTPVIEGGD VKEPLRDRVL
0841 GRVTAEDVLK PGTADILVPR NTLLHEQWCD LLEENSVDAV KVRSVVSCDT DFGVCAHCYG RDLARGHIIN
0911 KGEAIGVIAA QSIGEPGTQL TMRTFHIGGA ASRAAAESSI QVKNKGSIKL SNVKSVVNSS GKLVITSRNT
0981 ELKLIDEFGR TKESYKVPYG AVLAKGDGEQ VAGGETVANW DPHTMPVITE VSGFVRFTDM IDGQTITRQT
1051 DELTGLSSLV VLDSAERTAG GKDLRPALKI VDAQGNDVLI PGTDMPAQYF LPGKAIVQLE DGVQISSGDT
1121 LARIPQESGG TKDITGGLPR VADLFEARRP KEPAILAEIS GIVSFGKETK GKRRLVITPV DGSDPYEEMI
1191 PKWRQLNVFE GERVERGDVI SDGPEAPHDI LRLRGVHAVT RYIVNEVQDV YRLQGVKIND KHIEVIVRQM
1261 LRKATIVNAG SSDFLEGEQV EYSRVKIANR ELEANGKVGA TYSRDLLGIT KASLATESFI SAASFQETTR
1331 VLTEAAVAGK RDELRGLKEN VIVGRLIPAG TGYAYHQDRM RRRAAGEAPA APQVTAEDAS ASLAELLNAG
1401 LGGSDNE
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 AACATCGAAC TGGAAGACGA GTAATTCTCG
-070 CTCAAACAGG TCACTGCTGT CGGGTTAAAA CCCGGCAGCG GATTGTGCTA ACTCCGACGG GAGCAAATCC
0001 gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc aaaattgctc
0071 tggcttcgcc agacatgatc cgttcatggt ctttcggtga agttaaaaag ccggaaacca tcaactaccg
0141 tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc
0211 ctgtgcggta agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
0281 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca tctggttcct
0351 gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac
0421 tttgaatcct atgtggttat cgaaggcggt atgaccaacc tggaacgtca gcagatcctg actgaagagc
0491 agtatctgga cgcgctggaa gagttcggtg acgaattcga cgcgaagatg ggggcggaag caatccaggc
0561 tctgctgaag agcatggatc tggagcaaga gtgcgaacag ctgcgtgaag agctgaacga aaccaactcc
0631 gaaaccaagc gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
0701 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc cgctggatgg
0771 tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa
0841 cgtctgctgg atctggctgc gccggacatc atcgtacgta acgaaaaacg tatgctgcag gaagcggtag
0911 acgccctgct ggataacggt cgtcgcggtc gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt
0981 ggccgacatg atcaaaggta aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc
1051 ggtcgttctg taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcac
1121 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca ttaaagctgc
1191 gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg aagttatccg cgaacacccg
1261 gtactgctga accgtgcacc gactctgcac cgtctgggta tccaggcatt tgaaccggta ctgatcgaag
1331 gtaaagctat ccagctgcac ccgctggttt gtgcggcata taacgccgac ttcgatggtg accagatggc
1401 tgttcacgta ccgctgacgc tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc
1471 ctgtccccgg cgaacggcga accaatcatc gttccgtctc aggacgttgt actgggtctg tactacatga
1541 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag aacgtctgta
1611 tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac
1681 ggtgaattag tagcgaaaac cagcctgaaa gacacgactg ttggccgtgc cattctgtgg atgattgtac
1751 cgaaaggtct gccttactcc atcgtcaacc aggcgctggg taaaaaagca atctccaaaa tgctgaacac
1821 ctgctaccgc attctcggtc tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc
1891 tatgcagcgc gttctggtgc atctgttggt atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
1961 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa ctgcgggcga
2031 acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat ccaaagcgat gatggataac
2101 ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag agaagcaggt ttccttcaac agcatctaca
2171 tgatggccga ctccggtgcg cgtggttctg cggcacagat tcgtcagctt gctggtatgc gtggtctgat
2241 ggcgaagccg gatggctcca tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc
2311 cagtacttca tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcgaactccg
2381 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt gtggtaccca
2451 tgaaggtatc atgatgactc cggttatcga gggtggtgac gttaaagagc cgctgcgcga tcgcgtactg
2521 ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg ctgatatcct cgttccgcgc aacacgctgc
2591 tgcacgaaca gtggtgtgac ctgctggaag agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc
2661 ttgtgacacc gactttggtg tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac
2731 aagggtgaag caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
2801 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caagtgaaaa acaaaggtag
2871 catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg ttatcacttc ccgtaatact
2941 gaactgaaac tgatcgacga attcggtcgt actaaagaaa gctacaaagt accttacggt gcggtactgg
3011 cgaaaggcga tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt
3081 tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg atcgacggcc agaccattac gcgtcagacc
3151 gacgaactga ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
3221 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatc ccaggtaccg atatgccagc
3291 gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac agatcagctc tggtgacacc
3361 ctggcgcgta ttccgcagga atccggcggt accaaggaca tcaccggtgg tctgccgcgc gttgcggacc
3431 tgttcgaagc acgtcgtccg aaagagccgg caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa
3501 agaaaccaaa ggtaaacgtc gtctggttat caccccggta gacggtagcg atccgtacga agagatgatt
3571 ccgaaatggc gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
3641 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg ttaacgaagt
3711 acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg aagttatcgt tcgtcagatg
3781 ctgcgtaaag ctaccatcgt taacgcgggt agctccgact tcctggaagg cgaacaggtt gaatactctc
3851 gcgtcaagat cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca acttactccc gcgatctgct
3921 gggtatcacc aaagcgtctc tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc
3991 gtgctgaccg aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
4061 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg ctgcgggtga
4131 agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg cagaactgct gaacgcaggt
4201 ctgggcggtt ctgataacga gtaaTCGTTA ATCCGCAAAT AACGTAAAAA CCCGCTTCGG CGGGTTTTTT
4271 TATGGGGGGA GTTTAGGGAA AGAGCATTTG TCAGAATATT TAAGGAATTT CTGA
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