MG1655
W3110
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Gene Report : rpoB
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Mol Gen Genet. 1982;188(3):399-404.
Genetic studies on the beta subunit of Escherichia coli RNA polymerase. I. The effect of known, single amino acid substitutions in an essential protein.
Nene V, Glass RE. ( 6761548 )
     Deletion OCR10,11-11 (AK1-16 / BK2-16) (D)  ,  OCR10,11-16 (BK5-16 / AK7-16) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rpoB
Related strains
     Strains Search Search strains by gene name:  rpoB   Search strains by all related name:  rpoB b3987 ECK3978 ftsR groN JW3950 mbrD? nitB o1342 rif ron sdgB stl stv tabD tabG

General information  (Go to Linear View:)
 Gene Name rpoB  
 Alternative name b3987,ECK3978,ftsR,groN,JW3950,mbrD?,nitB,o1342,rif,ron,sdgB,stl,stv,tabD,tabG  
 Location, Length 4,179,268 - 4,183,296 (  +  ) ;   90.08 min ; 4029 (bp) ,   1342 (aa) Go to Linear View
 Product RNA polymerase, beta subunit  
 Operon Name rplKAJL-rpoBC  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006350 - transcription  
 Function enzyme; RNA synthesis, modification, DNA transcription  
 Gene Ontology GO:0003677 ; DNA binding ( rpoB )
GO:0003899 ; DNA-directed RNA polymerase activity ( rpoB )
GO:0005515 ; protein binding ( rpoB )
GO:0005737 ; cytoplasm ( rpoB )
GO:0006350 ; transcription ( rpoB )
GO:0006351 ; transcription, DNA-dependent ( rpoB )
GO:0016740 ; transferase activity ( rpoB )
GO:0016779 ; nucleotidyltransferase activity ( rpoB )
 PID 1790419  
 EC number
  (KEGG Pathway)
 
2.7.7.6  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene rpoB ( EG10894 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) RPOB_ECOLI ( P00575 ; P00576; P78242 )
    -  Protein name DNA-directed RNA polymerase beta chain  
    -  Synonyms EC 2.7.7.6, RNAP beta subunit, Transcriptase beta chain, RNA polymerase beta subunit  
    -  Gene name OrderedLocusNames=b3987, c4944, z5560, ECs4910, SF4060, S3675;  

 Linear View (Whole Mode)
View Location
   4170.0  –  4195.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP rpoB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain C, Crystal Structure Of Thermus Aquaticus Core Rna Polymerase- Includes Complete Structure With Side-Chains  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST DNA-directed RNA polymerase beta chain (RNAP beta subunit) (Transcriptase beta chain) (RNA polymerase beta subunit)  
         PSI-BLAST DNA-directed RNA polymerase beta chain
    nr     (database updated : 2007.02.20 )
         BLAST DNA-directed RNA polymerase beta subunit [Escherichia coli O157:H7 str. Sakai]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION    RGD    LEUCINE_ZIPPER    RNA_POL_BETA     

Other Cross-Reference
    COG COG0085K 
    EcoCyc rpoB 

MMBR References
Gene. 1980;11(3-4):367-73.
Nucleotide sequence of the proximal portion of the RNA polymerase beta subunit gene of Escherichia coli.
Delcuve G, Downing WL, Lewis HM, Dennis PP. ( 7011900 )
J Mol Biol. 1987;194(4):609-20.
Transcription products from the rplKAJL-rpoBC gene cluster.
Downing WL, Dennis PP. ( 2443706 )
Mol Microbiol. 1996;19(3):483-93.
Synthesis of the beta and beta' subunits of Escherichia coli RNA polymerase is autogenously regulated in vivo by both transcriptional and translational mechanisms.
Dykxhoorn DM, St Pierre R, Linn T. ( 8830239 )
Nature. 1968;220(164):276-7.
Mutations affecting RNA polymerase associated with rifampicin resistance in Escherichia coli.
Ezekiel DH, Hutchins JE. ( 4879326 )
Proc Natl Acad Sci U S A. 1971;68:2977-2981
Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase.
Georgopoulos, C.
J Mol Biol. 1972;65(2):259-72.
Relationship between the N function of bacteriophage lambda and host RNA polymerase.
Ghysen A, Pironio M. ( 5040359 )
J Bacteriol. 1976;127(1):32-9.
Wild-type and mutant in vitro products of an operon for ribonucleic acid polymerase subunits.
Austin S. ( 776928 )
J Mol Biol. 1970;49(1):263-7.
A new method for selecting RNA polymerase mutants.
Austin S, Scaife J. ( 4915864 )
Proc Natl Acad Sci U S A. 1980;77:2124-2128
Bacteriophage vehicle for the direct cloning of Escherichia coli promoter DNA sequences: feedback regulation of the rplJL-rpoBC operon.
Holowachuk, E. W., J. D. Friesen, and N. Fiil.
J Bacteriol. 1982;152(3):1022-32.
Insertions of transposon Tn5 into ribosomal protein PNA polymerase operons.
Hui I, Maltman K, Little R, Hastrup S, Johnsen M, Fiil N, Dennis P. ( 6292159 )
Mol Gen Genet. 1989;216(2-3):269-75.
Three rpoBC mutations that suppress the termination defects of rho mutants also affect the functions of nusA mutants.
Jin DJ, Gross CA. ( 2664452 )
Mol Gen Genet. 1980;179(3):489-96.
Autogenous and post-transcriptional regulation of Escherichia coli RNA polymerase synthesis in vitro.
Kajitani M, Fukuda R, Ishihama A. ( 6160369 )
J Bacteriol. 1994;176:1521-1523
Escherichia coli RNA polymerase mutants that enhance or diminish the SOS response constitutively expressed in the absence of RNase H1 activity.
Kogoma, T.
Proc Natl Acad Sci U S A. 1980;77:3331-3335
Attenuation and processing of RNA from the rplJL-rpoBC transcription unit of Escherichia coli.
Barry, G., C. Squires, and C. L. Squires.
Proc Natl Acad Sci U S A. 1979;76:4922-4926
Control features within the rplJL-rpoBC transcription unit of Escherichia coli.
Barry, G., C. L. Squires, and C. Squires.
Proc Natl Acad Sci U S A. 1991;88:6018-6022
A beta subunit mutation disrupting the catalytic function of Escherichia coli RNA polymerase.
Lee, J., M. Kashlev, S. Borukhov, and A. Goldfarb.
J Bacteriol. 1979;140(2):479-89.
Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome.
Linn T, Goman M, Scaife J. ( 159290 )
J Bacteriol. 1981;147(1):25-35.
Transcriptional and post-transcriptional control of ribosomal protein and ribonucleic acid polymerase genes.
Little R, Fiil NP, Dennis PP. ( 7016843 )
Mol Gen Genet. 1981;184(3):548-50.
Internal promoters of the rpoBC operon of Escherichia coli.
Ma JC, Newman AJ, Hayward RS. ( 6278264 )
Mol Gen Genet. 1980;177(3):527-33.
Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12.
Newman A, Hayward RS. ( 6246404 )
Eur J Biochem. 1982;116:621-629
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the B-subunit.
Ovchinnikov, Y. A., G. S. Monastyrskaya, V. V. Gubanov, S. O. Guryev, O. Y. Chertov, N. N. Modyanov, V. A. Grinkevich, I. A. Makarova, T. V. Marchenko, I. N. Polovnikova, V. M. Lipkin, and E. D. Sverdlov.
Proc Natl Acad Sci U S A. 1979;76:1697-1701
Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.
Post, L. E., G. D. Strycharz, M. Nomura, H. Lewis, and P. P. Dennis.
J Bacteriol. 1984;158(1):279-85.
Relative activities of the transcriptional regulatory sites in the rplKAJLrpoBC gene cluster of Escherichia coli.
Ralling G, Linn T. ( 6325390 )
J Mol Biol. 1991;222(2):189-96.
An Escherichia coli rpoB mutation that inhibits transcription of catabolite-sensitive operons.
Rockwell P, Gottesman ME. ( 1660071 )
Folia Microbiol. 1995;40:588-594.
In vivo cloning of a carboxy-terminal rpoB allele which confers altered transcriptional properties.
Rowland, G. C., P. P. Lim, and R. E. Glass.
Nature. 1969;223(210):1068-9.
Isolation and characterization of streptolydigin resistant RNA polymerase.
Schleif R. ( 4897821 )
J Mol Biol. 1991;218(1):23-31.
In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein-RNA polymerase gene cluster of Escherichia coli.
Steward KL, Linn T. ( 1825852 )
Nucleic Acids Res. 1992;20:4773-4779
Transcription frequency modulates the efficiency of an attenuator preceding the rpoBC RNA polymerase genes of Escherichia coli: possible autogenous control.
Steward, K. L., and T. Linn.
Mol Gen Genet. 1978;165(1):21-30.
Correlation between the serine sensitivity and the derepressibility of the ilv genes in Escherichia coli relA- mutants.
Uzan M, Danchin A. ( 362163 )
J Bacteriol. 1994;176:966-972
Thermoinducible filamentation in Escherichia coli due to an altered RNA polymerase beta subunit is suppressed by high levels of ppGpp.
Vinella, D., and R. D'Ari.
Proc Natl Acad Sci U S A. 1968;61(4):1313-9.
RNA polymerase mutants of Escherichia coli. I. Mutants resistant to streptovaricin.
Yura T, Igarashi K. ( 4387741 )
Mol Gen Genet. 1981;183(2):277-82.
In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli.
Bruckner R, Matzura H. ( 7035827 )
J Bacteriol. 1980;144(3):904-16.
Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced internal promoter mutant.
An G, Friesen JD. ( 7002914 )

Sequences
Amino acid
FASTA format
0001 MVYSYTEKKR IRKDFGKRPQ VLDVPYLLSI QLDSFQKFIE QDPEGQYGLE AAFRSVFPIQ SYSGNSELQY 
0071 VSYRLGEPVF DVQECQIRGV TYSAPLRVKL RLVIYEREAP EGTVKDIKEQ EVYMGEIPLM TDNGTFVING 
0141 TERVIVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR GSWLDFEFDP KDNLFVRIDR RRKLPATIIL 
0211 RALNYTTEQI LDLFFEKVIF EIRDNKLQME LVPERLRGET ASFDIEANGK VYVEKGRRIT ARHIRQLEKD 
0281 DVKLIEVPVE YIAGKVVAKD YIDESTGELI CAANMELSLD LLAKLSQSGH KRIETLFTND LDHGPYISET 
0351 LRVDPTNDRL SALVEIYRMM RPGEPPTREA AESLFENLFF SEDRYDLSAV GRMKFNRSLL REEIEGSGIL 
0421 SKDDIIDVMK KLIDIRNGKG EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVKERLS LGDLDTLMPQ 
0491 DMINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEITHKRRI SALGPGGLTR ERAGFEVRDV HPTHYGRVCP 
0561 IETPEGPNIG LINSLSVYAQ TNEYGFLETP YRKVTDGVVT DEIHYLSAIE EGNYVIAQAN SNLDEEGHFV 
0631 EDLVTCRSKG ESSLFSRDQV DYMDVSTQQV VSVGASLIPF LEHDDANRAL MGANMQRQAV PTLRADKPLV 
0701 GTGMERAVAV DSGVTAVAKR GGVVQYVDAS RIVIKVNEDE MYPGEAGIDI YNLTKYTRSN QNTCINQMPC 
0771 VSLGEPVERG DVLADGPSTD LGELALGQNM RVAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELACVS 
0841 RDTKLGPEEI TADIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTPK GETQLTPEEK LLRAIFGEKA 
0911 SDVKDSSLRV PNGVSGTVID VQVFTRDGVE KDKRALEIEE MQLKQAKKDL SEELQILEAG LFSRIRAVLV 
0981 AGGVEAEKLD KLPRDRWLEL GLTDEEKQNQ LEQLAEQYDE LKHEFEKKLE AKRRKITQGD DLAPGVLKIV 
1051 KVYLAVKRRI QPGDKMAGRH GNKGVISKIN PIEDMPYDEN GTPVDIVLNP LGVPSRMNIG QILETHLGMA 
1121 AKGIGDKINA MLKQQQEVAK LREFIQRAYD LGADVRQKVD LSTFSDEEVM RLAENLRKGM PIATPVFDGA 
1191 KEAEIKELLK LGDLPTSGQI RLYDGRTGEQ FERPVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG 
1261 GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVNG RTKMYKNIVD GNHQMEPGMP ESFNVLLKEI 
1331 RSLGINIELE DE

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             TTGCGGAAAG TGTTCCATTT TCCGGTCAAC 
-070 AAAATAGTGT TGCACAAACT GTCCGCTCAA TGGACAGATG GGTCGACTTG TCAGCGAGCT GAGGAACCCT 
0001 atggtttact cctataccga gaaaaaacgt attcgtaagg attttggtaa acgtccacaa gttctggatg 
0071 taccttatct cctttctatc cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta 
0141 tggtctggaa gctgctttcc gttccgtatt cccgattcag agctacagcg gtaattccga gctgcaatac 
0211 gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat ccgtggcgtg acctattccg 
0281 caccgctgcg cgttaaactg cgtctggtga tctatgagcg cgaagcgccg gaaggcaccg taaaagacat 
0351 taaagaacaa gaagtctaca tgggcgaaat tccgctcatg acagacaacg gtacctttgt tatcaacggt 
0421 actgagcgtg ttatcgtttc ccagctgcac cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa 
0491 cccactcttc gggtaaagtg ctgtataacg cgcgtatcat cccttaccgt ggttcctggc tggacttcga 
0561 attcgatccg aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac catcattctg 
0631 cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa agttatcttt gaaatccgtg 
0701 ataacaagct gcagatggaa ctggtgccgg aacgcctgcg tggtgaaacc gcatcttttg acatcgaagc 
0771 taacggtaaa gtgtacgtag aaaaaggccg ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac 
0841 gacgtcaaac tgatcgaagt cccggttgag tacatcgcag gtaaagtggt tgctaaagac tatattgatg 
0911 agtctaccgg cgagctgatc tgcgcagcga acatggagct gagcctggat ctgctggcta agctgagcca 
0981 gtctggtcac aagcgtatcg aaacgctgtt caccaacgat ctggatcacg gcccatatat ctctgaaacc 
1051 ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta ccgcatgatg cgccctggcg 
1121 agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa cctgttcttc tccgaagacc gttatgactt 
1191 gtctgcggtt ggtcgtatga agttcaaccg ttctctgctg cgcgaagaaa tcgaaggttc cggtatcctg 
1261 agcaaagacg acatcattga tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg 
1331 atatcgacca cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt tccgcgttgg 
1401 cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct gatgccacag 
1471 gatatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg ttccagccag ctgtctcagt 
1541 ttatggacca gaacaacccg ctgtctgaga ttacgcacaa acgtcgtatc tccgcactcg gcccaggcgg 
1611 tctgacccgt gaacgtgcag gcttcgaagt tcgagacgta cacccgactc actacggtcg cgtatgtcca 
1681 atcgaaaccc ctgaaggtcc gaacatcggt ctgatcaact ctctgtccgt gtacgcacag actaacgaat 
1751 acggcttcct tgagactccg tatcgtaaag tgaccgacgg tgttgtaact gacgaaattc actacctgtc 
1821 tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac tccaacttgg atgaagaagg ccacttcgta 
1891 gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg cgaccaggtt gactacatgg 
1961 acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct gatcccgttc ctggaacacg atgacgccaa 
2031 ccgtgcattg atgggtgcga acatgcaacg tcaggccgtt ccgactctgc gcgctgataa gccgctggtt 
2101 ggtactggta tggaacgtgc tgttgccgtt gactccggtg taactgcggt agctaaacgt ggtggtgtcg 
2171 ttcagtacgt ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg 
2241 tatcgacatc tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca gatgccgtgt 
2311 gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc gtccaccgac ctcggtgaac 
2381 tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg gaatggttac aacttcgaag actccatcct 
2451 cgtatccgag cgtgttgttc aggaagaccg tttcaccacc atccacattc aggaactggc gtgtgtgtcc 
2521 cgtgacacca agctgggtcc ggaagagatc accgctgaca tcccgaacgt gggtgaagct gcgctctcca 
2591 aactggatga atccggtatc gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt 
2661 aacgccgaaa ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg tgagaaagcc 
2731 tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac ggttatcgac gttcaggtct 
2801 ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga aatcgaagaa atgcagctca aacaggcgaa 
2871 gaaagacctg tctgaagaac tgcagatcct cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta 
2941 gccggtggcg ttgaagctga gaagctcgac aaactgccgc gcgatcgctg gctggagctg ggcctgacag 
3011 acgaagagaa acaaaatcag ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa 
3081 gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac cgggcgtgct gaagattgtt 
3151 aaggtatatc tggcggttaa acgccgtatc cagcctggtg acaagatggc aggtcgtcac ggtaacaagg 
3221 gtgtaatttc taagatcaac ccgatcgaag atatgcctta cgatgaaaac ggtacgccgg tagacatcgt 
3291 actgaacccg ctgggcgtac cgtctcgtat gaacatcggt cagatcctcg aaacccacct gggtatggct 
3361 gcgaaaggta tcggcgacaa gatcaacgcc atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat 
3431 tcatccagcg tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga 
3501 agaagttatg cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt cgacggtgcg 
3571 aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc cggtcagatc cgcctgtacg 
3641 atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt tggttacatg tacatgctga aactgaacca 
3711 cctggtcgac gacaagatgc acgcgcgttc caccggttct tacagcctgg ttactcagca gccgctgggt 
3781 ggtaaggcac agttcggtgg tcagcgtttc ggggagatgg aagtgtgggc gctggaagca tacggcgcag 
3851 catacaccct gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa 
3921 catcgtggac ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt gaaagagatt 
3991 cgttcgctgg gtatcaacat cgaactggaa gacgagtaaT TCTCGCTCAA ACAGGTCACT GCTGTCGGGT 
4061 TAAAACCCGG CAGCGGATTG TGCTAACTCC GACGGGAGCA AATCCGTGAA AGATTTATTA AAGTTTCTG