MG1655
W3110
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Gene Report : rplK
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR10,11-11 (AK1-16 / BK2-16) (D)  ,  OCR10,11-16 (BK5-16 / AK7-16) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rplK
Related strains
     Strains Search Search strains by gene name:  rplK   Search strains by all related name:  rplK b3983 ECK3974 JW3946 o142 relC

General information  (Go to Linear View:)
 Gene Name rplK  
 Alternative name b3983,ECK3974,JW3946,o142,relC  
 Location, Length 4,176,470 - 4,176,898 (  +  ) ;   90.02 min ; 429 (bp) ,   142 (aa) Go to Linear View
 Product 50S ribosomal subunit protein L11  
 Operon Name rplKAJL-rpoBC  
 Note GO_component: GO:0005737 - cytoplasm; GO_function: GO:0003735 - structural constituent of ribosome; GO_process: GO:0006412 - translation  
 Function structural component; Ribosomal proteins - synthesis, modification  
 Gene Ontology GO:0003723 ; RNA binding ( rplK )
GO:0003735 ; structural constituent of ribosome ( rplK )
GO:0005515 ; protein binding ( rplK )
GO:0005622 ; intracellular ( rplK )
GO:0005840 ; ribosome ( rplK )
GO:0006412 ; translation ( rplK )
GO:0019843 ; rRNA binding ( rplK )
GO:0030529 ; ribonucleoprotein complex ( rplK )
 PID 2367334  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene rplK ( EG10872 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) RL11_ECOLI ( P02409 ; P76778 )
    -  Protein name 50S ribosomal protein L11.  
    -  Synonyms  
    -  Gene name t3478; ORFNames=STMF1.8;  

 Linear View (Whole Mode)
View Location
   4165.0  –  4190.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP rplK (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain L, Crystal Structure Of The Ribosome At 5.5 A Resolution. This File, 1giy, Contains The 50s Ribosome Subunit. The 30s Ribosome Subunit, Three Trna, And Mrna Molecules Are In The File 1gix  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 50S ribosomal protein L11  
         PSI-BLAST 50S ribosomal protein L11
    nr     (database updated : 2007.02.20 )
         BLAST 50S ribosomal subunit protein L11 [Shigella boydii Sb227]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    RIBOSOMAL_L11     

Other Cross-Reference
    EcoCyc rplK 

MMBR References
J Mol Biol. 1987;194(4):609-20.
Transcription products from the rplKAJL-rpoBC gene cluster.
Downing WL, Dennis PP. ( 2443706 )
J Bacteriol. 1982;152(3):1022-32.
Insertions of transposon Tn5 into ribosomal protein PNA polymerase operons.
Hui I, Maltman K, Little R, Hastrup S, Johnsen M, Fiil N, Dennis P. ( 6292159 )
J Bacteriol. 1979;140(2):479-89.
Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome.
Linn T, Goman M, Scaife J. ( 159290 )
J Bacteriol. 1981;147(1):25-35.
Transcriptional and post-transcriptional control of ribosomal protein and ribonucleic acid polymerase genes.
Little R, Fiil NP, Dennis PP. ( 7016843 )
Nucleic Acids Symp Ser. 1995;114:70-72.
Mutations at three sites in the Escherichia coli 23S ribosomal RNA binding region for protein L11 cause UGA-specific suppression and conditional lethality.
Murgola, E. J., W. Xu, and A. L. Arkov.
Mol Gen Genet. 1980;177(3):527-33.
Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12.
Newman A, Hayward RS. ( 6246404 )
Proc Natl Acad Sci U S A. 1979;76:1697-1701
Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli.
Post, L. E., G. D. Strycharz, M. Nomura, H. Lewis, and P. P. Dennis.
J Bacteriol. 1984;158(1):279-85.
Relative activities of the transcriptional regulatory sites in the rplKAJLrpoBC gene cluster of Escherichia coli.
Ralling G, Linn T. ( 6325390 )
J Mol Biol. 1991;218(1):23-31.
In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein-RNA polymerase gene cluster of Escherichia coli.
Steward KL, Linn T. ( 1825852 )
Gene. 1979;6(4):331-65.
Escherichia coli RNA polymerase binding and initiation of transcription on fragments of lambda rifd 18 DNA containing promoters for lambda genes and for rrnB, tufB, rplC,A, rplJ,L, and rpoB,C genes.
Taylor WE, Burgess RR. ( 159206 )
Nucleic Acids Res. 1987;15:3085-3096
Translational regulation of the L11 ribosomal protein operon of Escherichia coli: mutations that define the target site for repression by L1.
Thomas, M. S., and M. Nomura.
Mol Gen Genet. 1981;183(2):277-82.
In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli.
Bruckner R, Matzura H. ( 7035827 )
J Bacteriol. 1980;144(3):904-16.
Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced internal promoter mutant.
An G, Friesen JD. ( 7002914 )
J Bacteriol. 1978;136(3):994-1001.
Kasugamycin-dependent mutants of Escherichia coli.
Dabbs ER. ( 363701 )
Mol Gen Genet. 1977;150(3):293-9.
A new technique for selection of sensitive and auxotrophic mutants of E. coli: isolation of a strain sensitive to an excess of one-carbon metabolites.
Danchin A. ( 321938 )

Sequences
Amino acid
FASTA format
0001 MAKKVQAYVK LQVAAGMANP SPPVGPALGQ QGVNIMEFCK AFNAKTDSIE KGLPIPVVIT VYADRSFTFV 
0071 TKTPPAAVLL KKAAGIKSGS GKPNKDKVGK ISRAQLQEIA QTKAADMTGA DIEAMTRSIE GTARSMGLVV 
0141 ED

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             ATTTCGCGCC TTTTGTTTTT ATGGGCCTTG 
-070 CCCGTAAAAC GATTTTTTAT ATCACGGGGA GCCTCTCAGA GGCGTTATTA CCCAACTTGA GGAATTTATA 
0001 atggctaaga aagtacaagc ctatgtcaag ctgcaggttg cagctggtat ggctaacccg agtccgccag 
0071 taggtccggc tctgggtcag cagggcgtaa acatcatgga attctgcaaa gcgttcaacg caaaaactga 
0141 ttccatcgaa aaaggtctgc cgattccggt agtaatcacc gtttacgctg accgttcttt cactttcgtt 
0211 accaagaccc cgccggcagc agttctgctg aaaaaagcgg ctggtatcaa gtctggttcc ggtaagccga 
0281 acaaagacaa agtgggtaaa atttcccgcg ctcagctgca ggaaatcgcg cagaccaaag ctgccgacat 
0351 gactggtgcc gacattgaag cgatgactcg ctccatcgaa ggtactgcac gttccatggg cctggtagtg 
0421 gaggactaaG AAATGGCTAA ACTGACCAAG CGCATGCGTG TTATCCGCGA GAAAGTTGAT GCAACCAAAC 
0491 AGTACGACAT CAACGAAGCT ATCGCACTGC TGAAAGAGC