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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCR10,11-16 (BK5-16 / AK7-16)
(D)
,
OCR10,11-7
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
btuB
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Related strains
|
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Strains Search
|
Search strains by gene name:
btuB
Search strains by all related name:
btuB b3966 bfe cer dcrC ECK3958 JW3938 o614
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General information (Go to Linear View: )
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Gene Name
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btuB
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Alternative name
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b3966,bfe,cer,dcrC,ECK3958,JW3938,o614
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Location, Length
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4,161,662 - 4,163,506
(
+
)
;
89.70
min
;
1845
(bp) ,
614
(aa)
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Product
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vitamin B12/cobalamin outer membrane transporter
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Operon Name
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btuB-murI
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Note
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outer membrane receptor for transport of vitamin B12, E colicins, and bacteriophage BF23; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0009279 - cell outer membrane
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Function
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membrane; Outer membrane constituents
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Gene Ontology
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GO:0004872
;
receptor activity ( btuB )
GO:0005215
;
transporter activity ( btuB )
GO:0005509
;
calcium ion binding ( btuB )
GO:0005515
;
protein binding ( btuB )
GO:0006810
;
transport ( btuB )
GO:0009279
;
cell outer membrane ( btuB )
GO:0009597
;
detection of virus ( btuB )
GO:0015235
;
cobalamin transporter activity ( btuB )
GO:0015889
;
cobalamin transport ( btuB )
GO:0016020
;
membrane ( btuB )
GO:0016021
;
integral to membrane ( btuB )
GO:0019867
;
outer membrane ( btuB )
GO:0046718
;
entry of virus into host cell ( btuB )
GO:0046872
;
metal ion binding ( btuB )
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PID
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1790405
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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btuB
(
EG10126
)
btuF
(
EG12334
)
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Number of removed
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20 aa cleaved
22 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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BTUB_ECOLI
(
P06129
)
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- Protein name
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Vitamin B12 receptor precursor.
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- Synonyms
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- Gene name
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Name=btuB; Synonyms=bfe, cer, dcrC; OrderedLocusNames=b3966;
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MMBR References
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J Biol Chem. 1988;263:14224-14230
Altered binding and transport of vitamin B12 resulting from insertion mutations in the Escherichia coli btuB gene.
Gudmundsdottir, A., C. Bradbeer, and R. J. Kadner.
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J Bacteriol. 1989;171(12):6526-33.
Point mutations in a conserved region (TonB box) of Escherichia coli outer membrane protein BtuB affect vitamin B12 transport.
Gudmundsdottir A, Bell PE, Lundrigan MD, Bradbeer C, Kadner RJ.
(
2687240
)
|
C R Acad Sci III. 1986;303(3):49-54.
Overlapping reading frames in Escherichia coli.The two promoters and the first 65 nucleotides of a gene related to the rrnB operon are localized in the coding sequence of btuB.
Aufrere R, Tempete M, Bohin JP.
(
3019489
)
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Mol Gen Genet. 1986;205(2):358-65.
Regulation of expression of the gene for vitamin B12 receptor cloned on a multicopy plasmid in Escherichia coli.
Aufrere R, Tempete M, Bohin JP.
(
3027510
)
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J Bacteriol. 1985;161(3):904-8.
Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli.
Heller K, Kadner RJ.
(
3882670
)
|
Mol Microbiol. 1995;15(2):381-93.
Structure-function analysis of the vitamin B12 receptor of Escherichia coli by means of informational suppression.
Hufton SE, Ward RJ, Bunce NA, Armstrong JT, Fletcher AJ, Glass RE.
(
7746157
)
|
J Bacteriol. 1973;115(2):514-21.
Transport of vitamin B12 in Escherichia coli: genetic studies.
Kadner RJ, Liggins GL.
(
4579870
)
|
J Bacteriol. 1995;177:6810-6819
Sequences of the Escherichia coli BtuB protein essential for its insertion and function in the outer membrane.
Lathrop, J. T., B. Y. Wei, G. A. Touchie, and R. J. Kadner.
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J Bacteriol. 1996;178:5309-5315
Genetic control of the resistance to phage C1 of Escherichia coli K-12.
Likhacheva, N. A., V. V. Samsonov, V. V. Samsonov, and S. P. Sineoky.
|
Gene. 1979;8(1):25-34.
Clustered arg genes on a BamHI segment of the Escherichia coli chromosome.
Moran MC, Mazaitis AJ, Vogel RH, Vogel HJ.
(
231541
)
|
Mol Gen Genet. 1971;113(2):154-6.
Genetic analysis of Escherichia coli K12 mutants resistant to bacteriophage BF23 and the E-group colicins.
Buxton RS.
(
4944012
)
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| Sequences |
Amino acid
FASTA format
|
0001 MIKKASLLTA CSVTAFSAWA QDTSPDTLVV TANRFEQPRS TVLAPTTVVT RQDIDRWQST SVNDVLRRLP
0071 GVDITQNGGS GQLSSIFIRG TNASHVLVLI DGVRLNLAGV SGSADLSQFP IALVQRVEYI RGPRSAVYGS
0141 DAIGGVVNII TTRDEPGTEI SAGWGSNSYQ NYDVSTQQQL GDKTRVTLLG DYAHTHGYDV VAYGNTGTQA
0211 QTDNDGFLSK TLYGALEHNF TDAWSGFVRG YGYDNRTNYD AYYSPGSPLL DTRKLYSQSW DAGLRYNGEL
0281 IKSQLITSYS HSKDYNYDPH YGRYDSSATL DEMKQYTVQW ANNVIVGHGS IGAGVDWQKQ TTTPGTGYVE
0351 DGYDQRNTGI YLTGLQQVGD FTFEGAARSD DNSQFGRHGT WQTSAGWEFI EGYRFIASYG TSYKAPNLGQ
0421 LYGFYGNPNL DPEKSKQWEG AFEGLTAGVN WRISGYRNDV SDLIDYDDHT LKYYNEGKAR IKGVEATANF
0491 DTGPLTHTVS YDYVDARNAI TDTPLLRRAK QQVKYQLDWQ LYDFDWGITY QYLGTRYDKD YSSYPYQTVK
0561 MGGVSLWDLA VAYPVTSHLT VRGKIANLFD KDYETVYGYQ TAGREYTLSG SYTF
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CAAGCCCGAA GACCTGCCGG CCAACGTCGC
-070 ATCTGGTTCT CATCATCGCG TAATATTGAT GAAACCTGCG GCATCCTTCT TCTATTGTGG ATGCTTTACA
0001 atgattaaaa aagcttcgct gctgacggcg tgttccgtca cggcattttc cgcttgggca caggatacca
0071 gcccggatac tctcgtcgtt actgctaacc gttttgaaca gccgcgcagc actgtgcttg caccaaccac
0141 cgttgtgacc cgtcaggata tcgaccgctg gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg
0211 ggcgtcgata tcacccaaaa cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca
0281 gtcatgtgtt ggtgttaatt gatggcgtac gcctgaatct ggcgggggtg agtggttctg ccgaccttag
0351 ccagttccct attgcgcttg tccagcgtgt tgaatatatc cgtgggccgc gctccgctgt ttatggttcc
0421 gatgcaatag gcggggtggt gaatatcatc acgacgcgcg atgaacccgg aacggaaatt tcagcagggt
0491 ggggaagcaa tagttatcag aactatgatg tctctacgca gcaacaactg ggggataaga cacgggtaac
0561 gctgttgggc gattatgccc atactcatgg ttatgatgtt gttgcctatg gtaataccgg aacgcaagcg
0631 cagacagata acgatggttt tttaagtaaa acgctttatg gcgcgctgga gcataacttt actgatgcct
0701 ggagcggctt tgtgcgcggc tatggctatg ataaccgtac caattatgac gcgtattatt ctcccggttc
0771 accgttgctc gatacccgta aactctatag ccaaagttgg gacgccgggc tgcgctataa cggcgaactg
0841 attaaatcac aactcattac cagctatagc catagcaaag attacaacta cgatccccat tatggtcgtt
0911 atgattcgtc ggcgacgctc gatgagatga agcaatacac cgtccagtgg gcaaacaatg tcatcgttgg
0981 tcacggtagt attggtgcgg gtgtcgactg gcagaaacag actacgacgc cgggtacagg ttatgttgag
1051 gatggatatg atcaacgtaa taccggcatc tatctgaccg ggctgcaaca agtcggcgat tttacctttg
1121 aaggcgcagc acgcagtgac gataactcac agtttggtcg tcatggaacc tggcaaacca gcgccggttg
1191 ggaattcatc gaaggttatc gcttcattgc ttcctacggg acatcttata aggcaccaaa tctggggcaa
1261 ctgtatggct tctacggaaa tccgaatctg gacccggaga aaagcaaaca gtgggaaggc gcgtttgaag
1331 gcttaaccgc tggggtgaac tggcgtattt ccggatatcg taacgatgtc agtgacttga tcgattatga
1401 tgatcacacc ctgaaatatt acaacgaagg gaaagcgcgg attaagggcg tcgaggcgac cgccaatttt
1471 gataccggac cactgacgca tactgtgagt tatgattatg tcgatgcgcg caatgcgatt accgacacgc
1541 cgttgttacg ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact tcgactgggg
1611 tattacttat cagtatttag gcactcgcta tgataaggat tactcatctt atccttatca aaccgttaaa
1681 atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc cggtcacctc tcacctgaca gttcgtggta
1751 aaatagccaa cctgttcgac aaagattatg agacagtcta tggctaccaa actgcaggac gggaatacac
1821 cttgtctggc agctacacct tctgaACCAC GTCCCACCGT GCTGGTGTTT GACTCCGGCG TCGGTGGGTT
1891 GTCGGTCTAT GACGAGATCC GGCATCTCTT ACCGGATCTC CATTACATTT ATGCT
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