MG1655
W3110
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Gene Report : proC
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR38 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  proC
Related strains
     Strains Search Search strains by gene name:  proC   Search strains by all related name:  proC b0386 ECK0381 JW0377 pro pro-2 pro-3 Pro2

General information  (Go to Linear View:)
 Gene Name proC  
 Alternative name b0386,ECK0381,JW0377,pro,pro-2,pro-3,Pro2  
 Location, Length 404,059 - 404,868 (  -  ) ;   8.71 min ; 810 (bp) ,   269 (aa) Go to Linear View
 Product pyrroline-5-carboxylate reductase, NAD(P)-binding  
 Operon Name proC  
 Note pyrroline-5-carboxylate reductase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006561 - proline biosynthetic process  
 Function enzyme; Amino acid biosynthesis: Proline  
 Gene Ontology GO:0003824; catalytic activity ( proC )
GO:0004735; pyrroline-5-carboxylate reductase activity ( proC )
GO:0005488; binding ( proC )
GO:0005737; cytoplasm ( proC )
GO:0006118; electron transport ( proC )
GO:0006561; proline biosynthetic process ( proC )
GO:0008152; metabolic process ( proC )
GO:0008652; amino acid biosynthetic process ( proC )
GO:0016491; oxidoreductase activity ( proC )
 PID 1786585  
 EC number
  (KEGG Pathway)
 
1.5.1.2  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene proC ( EG10769 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) PROC_ECOLI ( P00373 )
    -  Protein name Pyrroline-5-carboxylate reductase  
    -  Synonyms EC 1.5.1.2, P5CR, P5C reductase  
    -  Gene name Name=proC; OrderedLocusNames=b0386, SF0322, S0330;  

 Linear View (Whole Mode)
View Location
   395.0  –  420.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP proC (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Human Pyrroline-5-Carboxylate Reductase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Pyrroline-5-carboxylate reductase (P5CR) (P5C reductase)  
         PSI-BLAST Glycerol-3-phosphate dehydrogenase [NAD
    nr     (database updated : 2007.02.20 )
         BLAST pyrroline-5-carboxylate reductase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    P5CR     

Other Cross-Reference
    COG COG0345E 
    EcoCyc proC 

MMBR References
Nucleic Acids Res. 1982;10:7701-7714
Escherichia coli delta-1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification.
Deutch, A. H., C. J. Smith, K. E. Rushlow, and P. J. Kretschmer.
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC. ( 6307829 )
J Gen Microbiol. 1979;113(1):155-64.
Isolation of haemin-requiring mutants of Escherichia coli K12.
McConville ML, Charles HP. ( 387909 )
Mol Gen Genet. 1982;185(3):510-2.
Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli.
Boidol W, Simonis M, Topert M, Siewert G. ( 6285149 )
J Bacteriol. 1986;165(1):226-32.
Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12.
DeFeyter RC, Pittard J. ( 3001024 )

Sequences
Amino acid
FASTA format
0001 MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG INAAESAQEV AQIADIIFAA 
0071 VKPGIMIKVL SEITSSLNKD SLVVSIAAGV TLDQLARALG HDRKIIRAMP NTPALVNAGM TSVTPNALVT 
0141 PEDTADVLNI FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF MFIEAMADAA VLGGMPRAQA YKFAAQAVMG 
0211 SAKMVLETGE HPGALKDMVC SPGGTTIEAV RVLEEKGFRA AVIEAMTKCM EKSEKLSKS

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             taatcctcta ttgtgtcgcg cttttgcctt 
-070 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag gagtgaggca 
0001 atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat tctcggcggt ctgattgcca 
0071 gcggtcaggt gcttccaggg caaatctggg tatacacccc ctccccggat aaagtcgccg ccctgcatga 
0141 ccagttcggc atcaacgccg cagaatcggc gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc 
0211 gttaaacctg gcatcatgat taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg 
0281 tttctattgc tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg 
0351 cgccatgccg aacactcccg cactggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc 
0421 ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt gctgagccga 
0491 tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtattt atgtttatcg aagcgatggc 
0561 cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt 
0631 tccgcaaaaa tggtgctgga aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag 
0701 gcaccaccat tgaagcggta cgcgtactgg aagagaaagg cttccgtgct gcagtgatcg aagcgatgac 
0771 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag gccgccactt 
0841 cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc gatttcaacc actcacgata