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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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phoA8(del)
,
OCR38
(D)
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|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
phoA
|
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Related strains
|
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Strains Search
|
Search strains by gene name:
phoA
Search strains by all related name:
phoA b0383 ECK0378 JW0374 psiA
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General information (Go to Linear View: )
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Gene Name
|
phoA
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Alternative name
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b0383,ECK0378,JW0374,psiA
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Location, Length
|
400,971 - 402,386
(
+
)
;
8.64
min
;
1416
(bp) ,
471
(aa)
|
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Product
|
bacterial alkaline phosphatase
|
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Operon Name
|
phoA-psiF
|
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Note
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alkaline phosphatase; start codon corrected; GO_component: GO:0042597 - periplasmic space; GO_process: GO:0006793 - phosphorus metabolic process
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Function
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enzyme; Central intermediary metabolism: Phosphorus compounds,1.8.1 metabolism; metabolism of other compounds; phosphorous metabolism,7.2 location of gene products; periplasmic space
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Gene Ontology
|
GO:0000287
;
magnesium ion binding ( phoA )
GO:0004035
;
alkaline phosphatase activity ( phoA )
GO:0005515
;
protein binding ( phoA )
GO:0008152
;
metabolic process ( phoA )
GO:0008270
;
zinc ion binding ( phoA )
GO:0016787
;
hydrolase activity ( phoA )
GO:0042597
;
periplasmic space ( phoA )
GO:0046872
;
metal ion binding ( phoA )
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PID
|
48994877
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EC number
(KEGG Pathway)
|
3.1.3.1
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
phoA
(
EG10727
)
|
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Number of removed
|
21 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
PPB_ECOLI
(
P00634
;
P77801; P78051; Q47041
)
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- Protein name
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Alkaline phosphatase precursor
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- Synonyms
|
EC 3.1.3.1, APase
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- Gene name
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Name=phoA; OrderedLocusNames=b0383;
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MMBR References
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J Biol Chem. 1990;265:896-901
Introduction of guest peptides into Escherichia coli alkaline phosphatase. Excision and purification of a dynorphin analogue from an active chimeric protein.
Freimuth, P. I., J. W. Taylor, and E. T. Kaiser.
|
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC.
(
6307829
)
|
J Bacteriol. 1982;151(2):529-33.
New pleiotropic alkaline phosphatase-negative mutants of Escherichia coli K-12.
Heyde M, Portalier R.
(
7047492
)
|
J Bacteriol. 1981;146(2):668-75.
Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro.
Inouye H, Michaelis S, Wright A, Beckwith J.
(
6260756
)
|
J Bacteriol. 1982;149(2):434-9.
Signal sequence of alkaline phosphatase of Escherichia coli.
Inouye H, Barnes W, Beckwith J.
(
7035431
)
|
EMBO J. 1992;11:57-62
Identification and characterization of an Escherichia coli gene required for the formation of correctly folded alkaline phosphatase, a periplasmic enzyme.
Kamitani, S., Y. Akiyama, and K. Ito.
|
Nucleic Acids Res. 1981;9:5671-5678
The nucleotide sequence of the promoter and the amino-terminal region of alkaline phosphatase structural gene (phoA) of Escherichia coli.
Kikuchi, Y., K. Yoda, M. Yamasaki, and G. Tamura.
|
J Bacteriol. 1981;146(2):660-7.
Cloning and characterization of the Escherichia coli gene coding for alkaline phosphatase.
Berg PE.
(
6260755
)
|
J Bacteriol. 1981;145(1):288-92.
Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA.
Sarthy A, Michaelis S, Beckwith J.
(
6450745
)
|
J Bacteriol. 1981;145(1):293-8.
Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome.
Sarthy A, Michaelis S, Beckwith J.
(
7007316
)
|
Nucleic Acids Res. 1986;14(21):8689
Sequence of the gene for alkaline phosphatase from Escherichia coli JM83.
Shuttleworth H, Taylor J, Minton N.
(
3537962
)
|
Mol Gen Genet. 1982;185(3):510-2.
Recombinant plasmids with genes for the biosynthesis of alkaline phosphatase of Escherichia coli.
Boidol W, Simonis M, Topert M, Siewert G.
(
6285149
)
|
J Bacteriol. 1961;81:835-836.
Mutants of Escherichia coli constitutive for alkaline phosphatase.
Torriani, A., and F. Rothman.
|
Genetics 1980;96:353-366
Mutants affected in alkaline phosphatase expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli.
Wanner, B. L., and P. Latterell.
|
J Bacteriol. 1981;146(1):93-101.
Use of bacteriophage transposon Mu d1 to determine the orientation for three proC-linked phosphate-starvation-inducible (psi) genes in Escherichia coli K-12.
Wanner BL, Wieder S, McSharry R.
(
6260750
)
|
Proc Natl Acad Sci U S A. 1981;78:3473-3477
Amino acid sequence of Escherichia coli alkaline phosphatase.
Bradshaw, R. A., F. Cancedda, L. H. Ericsson, P. A. Neumann, S. P. Piccoli, M. J. Schlesinger, K. Shriefer, and K. A. Walsh.
|
J Biol Chem. 1992;267:16244-16251
The importance of aspartate 327 for catalysis and zinc binding in Escherichia coli alkaline phosphatase.
Xu, X., and E. R. Kantrowitz.
|
Gene. 1986;44(1):121-5.
Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli.
Chang CN, Kuang WJ, Chen EY.
(
3533724
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MKQSTIALAL LPLLFTPVTK ARTPEMPVLE NRAAQGDITA PGGARRLTGD QTAALRDSLS DKPAKNIILL
0071 IGDGMGDSEI TAARNYAEGA GGFFKGIDAL PLTGQYTHYA LNKKTGKPDY VTDSAASATA WSTGVKTYNG
0141 ALGVDIHEKD HPTILEMAKA AGLATGNVST AELQDATPAA LVAHVTSRKC YGPSATSEKC PGNALEKGGK
0211 GSITEQLLNA RADVTLGGGA KTFAETATAG EWQGKTLREQ AQARGYQLVS DAASLNSVTE ANQQKPLLGL
0281 FADGNMPVRW LGPKATYHGN IDKPAVTCTP NPQRNDSVPT LAQMTDKAIE LLSKNEKGFF LQVEGASIDK
0351 QDHAANPCGQ IGETVDLDEA VQRALEFAKK EGNTLVIVTA DHAHASQIVA PDTKAPGLTQ ALNTKDGAVM
0421 VMSYGNSEED SQEHTGSQLR IAAYGPHAAN VVGLTDQTDL FYTMKAALGL K
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 AAAGTTAATC TTTTCAACAG CTGTCATAAA
-070 GTTGTCACGG CCGAGACTTA TAGTCGCTTT GTTTTTATTT TTTAATGTAT TTGTACATGG AGAAAATAAA
0001 gtgaaacaaa gcactattgc actggcactc ttaccgttac tgtttacccc tgtgacaaaa gcccggacac
0071 cagaaatgcc tgttctggaa aaccgggctg ctcagggcga tattactgca cccggcggtg ctcgccgttt
0141 aacgggtgat cagactgccg ctctgcgtga ttctcttagc gataaacctg caaaaaatat tattttgctg
0211 attggcgatg ggatggggga ctcggaaatt actgccgcac gtaattatgc cgaaggtgcg ggcggctttt
0281 ttaaaggtat agatgcctta ccgcttaccg ggcaatacac tcactatgcg ctgaataaaa aaaccggcaa
0351 accggactac gtcaccgact cggctgcatc agcaaccgcc tggtcaaccg gtgtcaaaac ctataacggc
0421 gcgctgggcg tcgatattca cgaaaaagat cacccaacga ttctggaaat ggcaaaagcc gcaggtctgg
0491 cgaccggtaa cgtttctacc gcagagttgc aggatgccac gcccgctgcg ctggtggcac atgtgacctc
0561 gcgcaaatgc tacggtccga gcgcgaccag tgaaaaatgt ccgggtaacg ctctggaaaa aggcggaaaa
0631 ggatcgatta ccgaacagct gcttaacgct cgtgccgacg ttacgcttgg cggcggcgca aaaacctttg
0701 ctgaaacggc aaccgctggt gaatggcagg gaaaaacgct gcgtgaacag gcacaggcgc gtggttatca
0771 gttggtgagc gatgctgcct cactgaattc ggtgacggaa gcgaatcagc aaaaacccct gcttggcctg
0841 tttgctgacg gcaatatgcc agtgcgctgg ctaggaccga aagcaacgta ccatggcaat atcgataagc
0911 ccgcagtcac ctgtacgcca aatccgcaac gtaatgacag tgtaccaacc ctggcgcaga tgaccgacaa
0981 agccattgaa ttgttgagta aaaatgagaa aggctttttc ctgcaagttg aaggtgcgtc aatcgataaa
1051 caggatcatg ctgcgaatcc ttgtgggcaa attggcgaga cggtcgatct cgatgaagcc gtacaacggg
1121 cgctggaatt cgctaaaaag gagggtaaca cgctggtcat agtcaccgct gatcacgccc acgccagcca
1191 gattgttgcg ccggatacca aagctccggg cctcacccag gcgctaaata ccaaagatgg cgcagtgatg
1261 gtgatgagtt acgggaactc cgaagaggat tcacaagaac ataccggcag tcagttgcgt attgcggcgt
1331 atggcccgca tgccgccaat gttgttggac tgaccgacca gaccgatctc ttctacacca tgaaagccgc
1401 tctggggctg aaataaAACC GCGCCCGGCA GTGAATTTTC GCTGCCGGGT GGTTTTTTTG CTGTTAGCAA
1471 CCAGACTTAA TGGCAGATCA CGGGCGCATA CGCTCATGGT TAAAAC
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