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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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OCR06-12
(D)
,
OCR06-18 (AK5-15 / BK6-15)
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
glnA
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Related strains
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Strains Search
|
Search strains by gene name:
glnA
Search strains by all related name:
glnA b3870 ECK3863 JW3841
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General information (Go to Linear View: )
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Gene Name
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glnA
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Alternative name
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b3870,ECK3863,JW3841
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Location, Length
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4,054,648 - 4,056,057
(
-
)
;
87.39
min
;
1410
(bp) ,
469
(aa)
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Product
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glutamine synthetase
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Operon Name
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glnALG
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006542 - glutamine biosynthetic process; GO_process: GO:0006807 - nitrogen compound metabolic process
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Function
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enzyme; Amino acid biosynthesis: Glutamine
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Gene Ontology
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GO:0000166
;
nucleotide binding ( glnA )
GO:0003824
;
catalytic activity ( glnA )
GO:0004356
;
glutamate-ammonia ligase activity ( glnA )
GO:0005624
;
membrane fraction ( glnA )
GO:0005737
;
cytoplasm ( glnA )
GO:0006542
;
glutamine biosynthetic process ( glnA )
GO:0006807
;
nitrogen compound metabolic process ( glnA )
GO:0009399
;
nitrogen fixation ( glnA )
GO:0016874
;
ligase activity ( glnA )
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PID
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1790301
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EC number
(KEGG Pathway)
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6.3.1.2
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
glnA
(
EG10383
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
)
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Entry name(Acc.no)
|
GLNA_ECOLI
(
P06711
)
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- Protein name
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Glutamine synthetase
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- Synonyms
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EC 6.3.1.2, Glutamate--ammonia ligase
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- Gene name
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OrderedLocusNames=b3870, c4819, z5406, ECs4792, SF3940, S3806;
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MMBR References
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J Bacteriol. 1982;150(3):1314-21.
Polarity in the glnA operon: suppression of the reg- phenotype by rho mutations.
Guterman SK, Roberts G, Tyler B.
(
6122677
)
|
Proc Natl Acad Sci U S A. 1981;78:3743-3747
Physical and genetic characterization of the glnA-glnG region of the Escherichia coli chromosome.
Backman, K., Y.-M. Chen, and B. Magasanik.
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J Bacteriol. 1981;146(1):260-8.
General method, using Mu-Mud1 dilysogens, to determine the direction of transcription of and generate deletions in the glnA region of Escherichia coli.
MacNeil D.
(
6111550
)
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J Bacteriol. 1982;150(3):1302-13.
Fine-structure deletion map and complementation analysis of the glnA-glnL-glnG region in Escherichia coli.
MacNeil T, MacNeil D, Tyler B.
(
6122676
)
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Biochimie. 1989;71(9-10):1005-12.
Regulation of transcription of the glnALG operon of Escherichia coli by protein phosphorylation.
Magasanik B.
(
2574599
)
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Mol Gen Genet. 1982;185:152-157.
Characterization of lambda glnA+ phages used as templates for in vitro synthesis of glutamine synthetase.
McFarland, N., L. McCarter, S. Artz, and S. Kustu.
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Nucleic Acids Res. 1987;15:2757-2770
The complete nucleotide sequence of the glnALG operon of Escherichia coli K12.
Miranda-Rios, J., R. Sanchez-Pescador, M. Urdea, and A. Covarrubias.
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Mol Gen Genet. 1979;175(1):77-87.
Characterization of lambda polA transducing phages; effective expression of the E. coli polA gene.
Murray NE, Kelley WS.
(
159999
)
|
J Bacteriol. 1982;150(1):202-13.
Complex glnA-glnL-glnG operon of Escherichia coli.
Pahel G, Rothstein DM, Magasanik B.
(
6120929
)
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Proc Natl Acad Sci U S A. 1985;82:1979-1983
Expression of glnA in Escherichia coli is regulated at tandem promoters.
Reitzer, L. J., and B. Magasanik.
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Gene. 1985;37(1-3):91-9.
Nucleotide sequence of the glnA-glnL intercistronic region of Escherichia coli.
Rocha M, Vazquez M, Garciarrubio A, Covarrubias AA.
(
2865194
)
|
Proc Natl Acad Sci U S A. 1980;77:7372-7376
Regulation of expression from the glnA promoter of Escherichia coli in the absence of glutamine synthetase.
Rothstein, D. M., G. Pahel, B. Tyler, and B. Magasanik.
|
J Biol Chem. 1986;261:10587-10591
Amino acid sequence of Escherichia coli glutamine synthetase deduced from the DNA nucleotide sequence.
Colombo, G., and J. J. Villafranca.
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Mol Gen Genet. 1983;190(1):171-5.
Nucleotide sequence of the glnA control region of Escherichia coli.
Covarrubias AA, Bastarrachea F.
(
6134228
)
|
Plasmid. 1980;3(2):150-64.
ColE1 hybrid plasmids containing Escherichia coli genes involved in the biosynthesis of glutamate and glutamine.
Covarrubias AA, Sanchez-Pescador R, Osorio A, Bolivar F, Bastarrachea F.
(
6152835
)
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| Sequences |
Amino acid
FASTA format
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0001 MSAEHVLTML NEHEVKFVDL RFTDTKGKEQ HVTIPAHQVN AEFFEEGKMF DGSSIGGWKG INESDMVLMP
0071 DASTAVIDPF FADSTLIIRC DILEPGTLQG YDRDPRSIAK RAEDYLRSTG IADTVLFGPE PEFFLFDDIR
0141 FGSSISGSHV AIDDIEGAWN SSTQYEGGNK GHRPAVKGGY FPVPPVDSAQ DIRSEMCLVM EQMGLVVEAH
0211 HHEVATAGQN EVATRFNTMT KKADEIQIYK YVVHNVAHRF GKTATFMPKP MFGDNGSGMH CHMSLSKNGV
0281 NLFAGDKYAG LSEQALYYIG GVIKHAKAIN ALANPTTNSY KRLVPGYEAP VMLAYSARNR SASIRIPVVS
0351 SPKARRIEVR FPDPAANPYL CFAALLMAGL DGIKNKIHPG EAMDKNLYDL PPEEAKEIPQ VAGSLEEALN
0421 ELDLDREFLK AGGVFTDEAI DAYIALRREE DDRVRMTPHP VEFELYYSV
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttggcacaga tttcgcttta tcttttttac
-070 ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgaccatgac caatccagga gagttaaagt
0001 atgtccgctg aacacgtact gacgatgctg aacgagcacg aagtgaagtt tgttgatttg cgcttcaccg
0071 atactaaagg taaagaacag cacgtcacta tccctgctca tcaggtgaat gctgaattct tcgaagaagg
0141 caaaatgttt gacggctcct cgattggcgg ctggaaaggc attaacgagt ccgacatggt gctgatgcca
0211 gacgcatcca ccgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
0281 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc cattgcgaag cgcgccgaag attacctgcg
0351 ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct tcctgttcga tgacatccgt
0421 ttcggatcat ctatctccgg ttcccacgtt gctatcgacg atatcgaagg cgcatggaac tcctccaccc
0491 aatacgaagg tggtaacaaa ggtcaccgtc cggcagtgaa aggcggttac ttcccggttc caccggtaga
0561 ctcggctcag gatattcgtt ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat
0631 caccacgaag tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
0701 acgaaattca gatctacaaa tatgttgtgc acaacgtagc gcaccgcttc ggtaaaaccg cgacctttat
0771 gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt ctctgtctaa aaacggcgtt
0841 aacctgttcg caggcgacaa atacgcaggt ctgtctgagc aggcgctgta ctacattggc ggcgtaatca
0911 aacacgctaa agcgattaac gccctggcaa acccgaccac caactcttat aagcgtctgg tcccgggcta
0981 tgaagcaccg gtaatgctgg cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct
1051 tctccgaaag cacgtcgtat cgaagtacgt ttcccggatc cggcagctaa cccgtacctg tgctttgctg
1121 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg acaaaaacct
1191 gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct ctctggaaga agcactgaac
1261 gaactggatc tggaccgcga gttcctgaaa gccggtggcg tgttcactga cgaagcaatt gatgcgtaca
1331 tcgctctgcg tcgcgaagaa gatgaccgcg tgcgtatgac tccgcatccg gtagagtttg agctgtacta
1401 cagcgtctaa gtgttttagt tgccgtggaa acttttcgcc tgtctctggc aggcctggga tcggtggcaa
1471 gcacatcacg ccggatgcga cgcaaatgcg tcttatccgg
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