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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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OCR06-12
(D)
,
OCR06-18 (AK5-15 / BK6-15)
(D)
,
DE(glnG-glnA)229
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
glnL
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Related strains
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Strains Search
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Search strains by gene name:
glnL
Search strains by all related name:
glnL b3869 ECK3862 glnR JW3840 ntrB
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General information (Go to Linear View: )
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Gene Name
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glnL
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Alternative name
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b3869,ECK3862,glnR,JW3840,ntrB
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Location, Length
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4,053,313 - 4,054,362
(
-
)
;
87.36
min
;
1050
(bp) ,
349
(aa)
|
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Product
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sensory histidine kinase in two-component regulatory system with GlnG
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Operon Name
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glnALG
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Note
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histidine protein kinase sensor for GlnG regulator (nitrogen regulator II, NRII); GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006542 - glutamine biosynthetic process; GO_process: GO:0006464 - protein modification process
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Function
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enzyme; Amino acid biosynthesis: Glutamine
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Gene Ontology
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GO:0000155
;
two-component sensor activity ( glnL )
GO:0000160
;
two-component signal transduction system (phosphorelay) ( glnL )
GO:0000166
;
nucleotide binding ( glnL )
GO:0004673
;
protein histidine kinase activity ( glnL )
GO:0004871
;
signal transducer activity ( glnL )
GO:0005524
;
ATP binding ( glnL )
GO:0006355
;
regulation of transcription, DNA-dependent ( glnL )
GO:0007165
;
signal transduction ( glnL )
GO:0009399
;
nitrogen fixation ( glnL )
GO:0016020
;
membrane ( glnL )
GO:0016301
;
kinase activity ( glnL )
GO:0016310
;
phosphorylation ( glnL )
GO:0016740
;
transferase activity ( glnL )
GO:0016772
;
transferase activity, transferring phosphorus-containing groups ( glnL )
GO:0018106
;
peptidyl-histidine phosphorylation ( glnL )
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PID
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1790300
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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glnL
(
EG10387
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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NTRB_ECOLI
(
P06712
)
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- Protein name
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Nitrogen regulation protein NR
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- Synonyms
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II, EC 2.7.3.-
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- Gene name
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OrderedLocusNames=b3869, z5405, ECs4791, SF3939, S3807;
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MMBR References
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J Bacteriol. 1993;175:7016-7023
Mutational analysis of the bacterial signal-transducing protein kinase/phosphatase nitrogen regulator II (NRII or NtrB).
Atkinson, M. R., and A. J. Ninfa.
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Proc Natl Acad Sci U S A. 1981;78:3743-3747
Physical and genetic characterization of the glnA-glnG region of the Escherichia coli chromosome.
Backman, K., Y.-M. Chen, and B. Magasanik.
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J Bacteriol. 1982;150(3):1302-13.
Fine-structure deletion map and complementation analysis of the glnA-glnL-glnG region in Escherichia coli.
MacNeil T, MacNeil D, Tyler B.
(
6122676
)
|
Mol Gen Genet. 1982;188(2):325-33.
The products of glnL and glnG are bifunctional regulatory proteins.
MacNeil T, Roberts GP, MacNeil D, Tyler B.
(
6130464
)
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Biochimie. 1989;71(9-10):1005-12.
Regulation of transcription of the glnALG operon of Escherichia coli by protein phosphorylation.
Magasanik B.
(
2574599
)
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Proc Natl Acad Sci U S A. 1981;78:2135-2139
Nitrogen regulatory locus "glnR" of enteric bacteria is composed of cistrons ntrB and ntrC: identification of their protein products.
McFarland, N., L. McCarter, S. Artz, and S. Kustu.
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Mol Gen Genet. 1982;185:152-157.
Characterization of lambda glnA+ phages used as templates for in vitro synthesis of glutamine synthetase.
McFarland, N., L. McCarter, S. Artz, and S. Kustu.
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Nucleic Acids Res. 1987;15:2757-2770
The complete nucleotide sequence of the glnALG operon of Escherichia coli K12.
Miranda-Rios, J., R. Sanchez-Pescador, M. Urdea, and A. Covarrubias.
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Mol Gen Genet. 1986;205(2):260-9.
Cloning and complete nucleotide sequence of the Escherichia coli glutamine permease operon (glnHPQ).
Nohno T, Saito T, Hong JS.
(
3027504
)
|
J Bacteriol. 1982;150(1):202-13.
Complex glnA-glnL-glnG operon of Escherichia coli.
Pahel G, Rothstein DM, Magasanik B.
(
6120929
)
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Proc Natl Acad Sci U S A. 1985;82:1979-1983
Expression of glnA in Escherichia coli is regulated at tandem promoters.
Reitzer, L. J., and B. Magasanik.
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Gene. 1985;37(1-3):91-9.
Nucleotide sequence of the glnA-glnL intercistronic region of Escherichia coli.
Rocha M, Vazquez M, Garciarrubio A, Covarrubias AA.
(
2865194
)
|
J Bacteriol. 1984;160(1):379-84.
Identification and regulation of the glnL operator-promoter of the complex glnALG operon of Escherichia coli.
Ueno-Nishio S, Mango S, Reitzer LJ, Magasanik B.
(
6148334
)
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J Bacteriol. 1982;150(1):214-20.
Characterization of a gene, glnL, the product of which is involved in the regulation of nitrogen utilization in Escherichia coli.
Chen YM, Backman K, Magasanik B.
(
6120930
)
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| Sequences |
Amino acid
FASTA format
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0001 MATGTQPDAG QILNSLINSI LLIDDNLAIH YANPAAQQLL AQSSRKLFGT PLPELLSYFS LNIELMQESL
0071 EAGQGFTDNE VTLVIDGRSH ILSVTAQRMP DGMILLEMAP MDNQRRLSQE QLQHAQQVAA RDLVRGLAHE
0141 IKNPLGGLRG AAQLLSKALP DPSLLEYTKV IIEQADRLRN LVDRLLGPQL PGTRVTESIH KVAERVVTLV
0211 SMELPDNVRL IRDYDPSLPE LAHDPDQIEQ VLLNIVRNAL QALGPEGGEI ILRTRTAFQL TLHGERYRLA
0281 ARIDVEDNGP GIPPHLQDTL FYPMVSGREG GTGLGLSIAR NLIDQHSGKI EFTSWPGHTE FSVYLPIRK
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 atgggctttt ttctccgtca attctctgat
-070 gcttcgcgct ttttatccgt aaaaagctat aatgcactaa aatggtgcaa cctgttcagg agactgcttt
0001 atggcaacag gcacgcagcc cgatgctggg cagatcctca actcgctgat taacagtatt ttgttaatcg
0071 atgacaacct ggcgatccat tacgccaacc ctgccgcgca acaactgctc gcccaaagct cccgcaaatt
0141 gtttggtaca ccgttaccgg aactgttgag ctacttctca ttaaatatcg agctgatgca agaaagtctg
0211 gaggcggggc aaggttttac cgataacgaa gtgacgctgg tcatcgacgg gcgctcgcat atcctttctg
0281 tgacggccca gcgtatgccg gacggcatga tcctgctgga gatggctccg atggataacc agcgccgctt
0351 aagtcaggaa cagctacagc acgcccagca ggttgctgcc cgtgatttag tgcgcggcct ggcacatgag
0421 attaaaaatc cgcttggcgg tttacgtggc gcggcgcagc tgctcagcaa agcgttacct gacccatcac
0491 tactcgaata taccaaagtg attatcgaac aggcggaccg gctgcgaaat ctggtcgacc gtctgttggg
0561 gccgcagctg cccggtacgc gcgttaccga aagtattcac aaagtggctg aacgcgtggt aacgctggtg
0631 tcgatggaac tgccggacaa cgtgcggttg attcgtgatt acgatcccag cctaccggaa ctggcgcacg
0701 acccggatca aattgaacag gtcttgctga atattgtgcg caatgcgcta caggcgctgg ggccggaagg
0771 cggtgaaatc attctgcgta cccgcaccgc gtttcaactg accttacacg gcgagcgcta ccggctggcg
0841 gcgcggattg atgtggaaga taacgggccg ggcattccgc ctcatttgca ggatacgctg ttttacccga
0911 tggtcagcgg ccgcgaaggt ggcaccgggc ttggcttatc catcgctcgt aatttgattg atcagcattc
0981 aggcaaaatt gaatttacca gttggccagg gcataccgag ttctcggttt acctgcctat caggaaataa
1051 aggtgacgtt tatgcaacga gggatagtct gggtagtcga tgacgatagt tccatccgtt gggtgcttga
1121 acgtgcgctc gctggggcag gtttaacctg
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