MG1655
W3110
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Gene Report : glnG
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion OCR06-12 (D)  ,  OCR06-18 (AK5-15 / BK6-15) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  glnG
Related strains
     Strains Search Search strains by gene name:  glnG   Search strains by all related name:  glnG b3868 ECK3861 gln glnT JW3839 ntrC

General information  (Go to Linear View:)
 Gene Name glnG  
 Alternative name b3868,ECK3861,gln,glnT,JW3839,ntrC  
 Location, Length 4,051,892 - 4,053,301 (  -  ) ;   87.33 min ; 1410 (bp) ,   469 (aa) Go to Linear View
 Product fused DNA-binding response regulator in two-component regulatory system with GlnL: responseregulator/sigma54 interaction protein  
 Operon Name glnALG  
 Note response regulator for gln (sensor glnL) (nitrogen regulator I, NRI); GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0006542 - glutamine biosynthetic process; GO_process: GO:0006350 - transcription  
 Function regulator; Amino acid biosynthesis: Glutamine  
 Gene Ontology GO:0000156 ; two-component response regulator activity ( glnG )
GO:0000160 ; two-component signal transduction system (phosphorelay) ( glnG )
GO:0000166 ; nucleotide binding ( glnG )
GO:0003677 ; DNA binding ( glnG )
GO:0003700 ; transcription factor activity ( glnG )
GO:0005524 ; ATP binding ( glnG )
GO:0005622 ; intracellular ( glnG )
GO:0006350 ; transcription ( glnG )
GO:0006355 ; regulation of transcription, DNA-dependent ( glnG )
GO:0008134 ; transcription factor binding ( glnG )
GO:0009399 ; nitrogen fixation ( glnG )
GO:0017111 ; nucleoside-triphosphatase activity ( glnG )
GO:0050906 ; detection of stimulus involved in sensory perception ( glnG )
 PID 1790299  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) NTRC_ECOLI ( P06713 )
    -  Protein name Nitrogen regulation protein NR  
    -  Synonyms I  
    -  Gene name OrderedLocusNames=b3868, z5404, ECs4790;  

 Linear View (Whole Mode)
View Location
   4040.0  –  4065.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP glnG (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Sigm54 Activator (Aaa+ Atpase) In The Inactive State  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Nitrogen regulation protein NR(I)  
         PSI-BLAST Nitrogen regulation protein NR(I)
    nr     (database updated : 2007.02.20 )
         BLAST GlnG [Escherichia coli O157:H7 str. Sakai]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    SIGMA54_INTERACT_1    SIGMA54_INTERACT_2    SIGMA54_INTERACT_3     

Other Cross-Reference
    COG COG2204T 
    EcoCyc glnG 

MMBR References
Proc Natl Acad Sci U S A. 1981;78:3743-3747
Physical and genetic characterization of the glnA-glnG region of the Escherichia coli chromosome.
Backman, K., Y.-M. Chen, and B. Magasanik.
J Bacteriol. 1981;146(1):260-8.
General method, using Mu-Mud1 dilysogens, to determine the direction of transcription of and generate deletions in the glnA region of Escherichia coli.
MacNeil D. ( 6111550 )
J Bacteriol. 1982;150(3):1302-13.
Fine-structure deletion map and complementation analysis of the glnA-glnL-glnG region in Escherichia coli.
MacNeil T, MacNeil D, Tyler B. ( 6122676 )
Mol Gen Genet. 1982;188(2):325-33.
The products of glnL and glnG are bifunctional regulatory proteins.
MacNeil T, Roberts GP, MacNeil D, Tyler B. ( 6130464 )
Biochimie. 1989;71(9-10):1005-12.
Regulation of transcription of the glnALG operon of Escherichia coli by protein phosphorylation.
Magasanik B. ( 2574599 )
Mol Gen Genet. 1982;185:152-157.
Characterization of lambda glnA+ phages used as templates for in vitro synthesis of glutamine synthetase.
McFarland, N., L. McCarter, S. Artz, and S. Kustu.
Nucleic Acids Res. 1987;15:2757-2770
The complete nucleotide sequence of the glnALG operon of Escherichia coli K12.
Miranda-Rios, J., R. Sanchez-Pescador, M. Urdea, and A. Covarrubias.
Proc Natl Acad Sci U S A. 1979;76:4544-4548
A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli.
Pahel, G., and B. Tyler.
J Bacteriol. 1982;150(1):202-13.
Complex glnA-glnL-glnG operon of Escherichia coli.
Pahel G, Rothstein DM, Magasanik B. ( 6120929 )

Sequences
Amino acid
FASTA format
0001 MQRGIVWVVD DDSSIRWVLE RALAGAGLTC TTFENGAEVL EALASKTPDV LLSDIRMPGM DGLALLKQIK 
0071 QRHPMLPVII MTAHSDLDAA VSAYQQGAFD YLPKPFDIDE AVALVERAIS HYQEQQQPRN VQLNGPTTDI 
0141 IGEAPAMQDV FRIIGRLSRS SISVLINGES GTGKELVAHA LHRHSPRAKA PFIALNMAAI PKDLIESELF 
0211 GHEKGAFTGA NTIRQGRFEQ ADGGTLFLDE IGDMPLDVQT RLLRVLADGQ FYRVGGYAPV KVDVRIIAAT 
0281 HQNLEQRVQE GKFREDLFHR LNVIRVHLPP LRERREDIPR LARHFLQVAA RELGVEAKLL HPETEAALTR 
0351 LAWPGNVRQL ENTCRWLTVM AAGQEVLIQD LPGELFESTV AESTSQMQPD SWATLLAQWA DRALRSGHQN 
0421 LLSEAQPELE RTLLTTALRH TQGHKQEAAR LLGWGRNTLT RKLKELGME

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             atttgattga tcagcattca ggcaaaattg 
-070 aatttaccag ttggccaggg cataccgagt tctcggttta cctgcctatc aggaaataaa ggtgacgttt 
0001 atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg 
0071 ctggggcagg tttaacctgt acgacgtttg agaacggcgc agaagtgctg gaggcgctgg cgagcaaaac 
0141 gccggatgtg ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa 
0211 cagcgccatc caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct 
0281 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc tggttgagcg 
0351 cgctatcagt cattaccagg aacagcagca gccgcgtaat gttcagctta acggcccaac gaccgatatc 
0421 atcggcgaag cgccagccat gcaggacgtg ttccgtatta tcggtcggct ttcgcgttct tctattagcg 
0491 tgctgattaa cggcgaatcc ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg 
0561 cgccaaagcg ccgtttatcg cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt 
0631 ggccacgaga aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg 
0701 gtacattatt cctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc 
0771 agacggtcag ttttaccgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat cgctgccact 
0841 caccagaatc tcgaacagcg agtgcaggaa ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta 
0911 tccgcgttca tctgccgccg ctgcgcgaac gtcgggaaga tattccccgt ctggcgcgcc attttttaca 
0981 ggttgccgcg cgcgaactgg gcgtagaagc gaagttactg catccggaaa ccgaagctgc tctgacgcgt 
1051 ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc 
1121 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta cttcgcaaat 
1191 gcaaccggac agctgggcga cgcttcttgc gcagtgggca gacagagcgc tgcgttccgg tcatcaaaat 
1261 ctgctttccg aagcgcagcc agagctggag cggacgttac tgacgaccgc gttgcgacat acgcaggggc 
1331 ataaacagga agcggcgcgg ctactcggct ggggccgcaa caccctgacg cgtaagttaa aagagctggg 
1401 gatggagtga ttcacagctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttacgcttta 
1471 ctgttccgat aagttcagta tgatcttgcc cggaaaacgg