|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Appl Environ Microbiol. 1995;61(7):2487-92.
Role of fadR and atoC(Con) mutations in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant pha+ Escherichia coli.
Rhie HG, Dennis D.
(
7618860
)
|
|
|
Deletion
|
OCR05
(D)
,
OCR05-12-1
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
fadA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
fadA
Search strains by all related name:
fadA b3845 ECK3837 JW5578 oldA
|
General information (Go to Linear View: )
|
|
Gene Name
|
fadA
|
|
Alternative name
|
b3845,ECK3837,JW5578,oldA
|
|
Location, Length
|
4,025,632 - 4,026,795
(
-
)
;
86.77
min
;
1164
(bp) ,
387
(aa)
|
|
Product
|
3-ketoacyl-CoA thiolase (thiolase I)
|
|
Operon Name
|
fadBA
|
|
Note
|
thiolase I; 3-ketoacyl-CoA thiolase; acetyl-CoA transferase; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0019395 - fatty acid oxidation
|
|
Function
|
enzyme; Degradation of small molecules: Fatty acids
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( fadA )
GO:0003988
;
acetyl-CoA C-acyltransferase activity ( fadA )
GO:0005737
;
cytoplasm ( fadA )
GO:0006629
;
lipid metabolic process ( fadA )
GO:0006631
;
fatty acid metabolic process ( fadA )
GO:0008152
;
metabolic process ( fadA )
GO:0008415
;
acyltransferase activity ( fadA )
GO:0016042
;
lipid catabolic process ( fadA )
GO:0016740
;
transferase activity ( fadA )
|
|
PID
|
48994986
|
EC number
(KEGG Pathway)
|
2.3.1.16
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
fadA
(
EG10278
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
THIK_ECOLI
(
P21151
;
P78130
)
|
|
- Protein name
|
3-ketoacyl-CoA thiolase
|
|
- Synonyms
|
EC 2.3.1.16, Fatty oxidation complex beta subunit, Beta-ketothiolase, Acetyl-CoA acyltransferase
|
|
- Gene name
|
Name=fadA; Synonyms=oldA; OrderedLocusNames=b3845;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
fadA
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain C, Fatty Acid Beta-Oxidation Multienzyme Complex From Pseudomonas Fragi, Form I (Native2)
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
3-ketoacyl-CoA thiolase
|
|
PSI-BLAST
|
3-ketoacyl-CoA thiolase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
acetyl-CoA acetyltransferase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
THIOLASE_1
THIOLASE_3
AA_TRNA_LIGASE_I
THIOLASE_2
|
|
MMBR References
|
J Bacteriol. 1990;172(11):6459-68.
Primary sequence of the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homology to eucaryotic enzymes.
DiRusso CC.
(
1699931
)
|
Nucleic Acids Res. 1990;18(16):4937
Nucleotide sequence of the fadA and fadB genes from Escherichia coli.
Nakahigashi K, Inokuchi H.
(
2204034
)
|
J Bacteriol. 1984;158(2):535-42.
Cloning, mapping, and expression of genes involved in the fatty acid-degradative multienzyme complex of Escherichia coli.
Spratt SK, Black PN, Ragozzino MM, Nunn WD.
(
6144665
)
|
J Biol Chem. 1983;258:9780-9785
The large subunit of the fatty acid oxidation complex from Escherichia coli is a multifunctional polypeptide. Evidence for the existence of a fatty acid oxidation operon (fadAB) in Escherichia coli.
Yang, S.-Y., and H. Schulz.
|
J Biol Chem. 1990;265:10424-10429
Nucleotide sequence of the fadA gene. Primary structure of 3-ketoacyl-coenzyme A thiolase from Escherichia coli and the structural organization of the fadAB operon.
Yang, S.-Y., X.-Y. Yang, G. Healy-Louie, H. Schulz, and M. Elzinga.
|
J Bacteriol. 1981;148(2):521-6.
Regulation of fatty acid degradation in Escherichia coli: analysis by operon fusion.
Clark D.
(
6271734
)
|
Science. 1992;257(5071):771-8.
Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes.
Daniels DL, Plunkett G 3rd, Burland VD, Blattner FR.
(
1379743
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MEQVVIVDAI RTPMGRSKGG AFRNVRAEDL SAHLMRSLLA RNPALEAAAL DDIYWGCVQQ TLEQGFNIAR
0071 NAALLAEVPH SVPAVTVNRL CGSSMQALHD AARMIMTGDA QACLVGGVEH MGHVPMSHGV DFHPGLSRNV
0141 AKAAGMMGLT AEMLARMHGI SREMQDAFAA RSHARAWAAT QSAAFKNEII PTGGHDADGV LKQFNYDEVI
0211 RPETTVEALA TLRPAFDPVN GMVTAGTSSA LSDGAAAMLV MSESRAHELG LKPRARVRSM AVVGCDPSIM
0281 GYGPVPASKL ALKKAGLSAS DIGVFEMNEA FAAQILPCIK DLGLIEQIDE KINLNGGAIA LGHPLGCSGA
0351 RISTTLLNLM ERKDVQFGLA TMCIGLGQGI ATVFERV
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ggaaggtctg cgtaataaag cgcgtcataa
-070 cgaaccgtac tatcctccgg ttgagccagc ccgtccggtt ggcgacctga aaacggctta aggagtcaca
0001 atggaacagg ttgtcattgt cgatgcaatt cgcaccccga tgggccgttc gaagggcggt gcttttcgta
0071 acgtgcgtgc agaagatctc tccgctcatt taatgcgtag cctgctggcg cgtaacccgg cgctggaagc
0141 ggcggccctc gacgatattt actggggttg tgtgcagcag acgctggagc agggttttaa tatcgcccgt
0211 aacgcggcgc tgctggcaga agtaccacac tctgtcccgg cggttaccgt taatcgcttg tgtggttcat
0281 ccatgcaggc actgcatgac gcagcacgaa tgatcatgac tggcgatgcg caggcatgtc tggttggcgg
0351 cgtggagcat atgggccatg tgccgatgag tcacggcgtc gattttcacc ccggcctgag ccgcaatgtc
0421 gccaaagcgg cgggcatgat gggcttaacg gcagaaatgc tggcgcgtat gcacggtatc agccgtgaaa
0491 tgcaggatgc ctttgccgcg cggtcacacg cccgcgcctg ggccgccacg cagtcggccg catttaaaaa
0561 tgaaatcatc ccgaccggtg gtcacgatgc cgacggcgtc ctgaagcagt ttaattacga cgaagtgatt
0631 cgcccggaaa ccaccgtgga agccctcgcc acgctgcgtc cggcgtttga tccagtaaac ggtatggtaa
0701 cggcgggcac atcttctgca ctttccgatg gcgcagctgc catgctggtg atgagtgaaa gccgcgccca
0771 tgaattaggt cttaagccgc gcgctcgtgt gcgttcgatg gcggtcgttg gttgtgaccc atcgattatg
0841 ggttacggcc cggttccggc ctcgaaactg gcgctgaaaa aagcggggct ttctgccagc gatatcggcg
0911 tgtttgaaat gaacgaagcc tttgccgcgc agatcctgcc atgtattaaa gatctgggac taattgagca
0981 gattgacgag aagatcaacc tcaacggtgg cgcgatcgcg ctgggtcatc cgctgggttg ttccggtgcg
1051 cgtatcagca ccacgctgct gaatctgatg gaacgcaaag acgttcagtt tggtctggcg acgatgtgta
1121 tcggtctggg tcagggtatt gcgacggtgt ttgagcgggt ttaaccactt gccggatgcg gcgtgaacgc
1191 cttatccggt ctaccgatcc ggcaccaatg taggcctgat aagacgcgcc agcgtcgcat caggcatcat
1261 gcac
|
