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PEC Original Annotations
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|
Essentiality
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Class
|
non-essential
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References (PMID)
|
J Biol Chem. 1994;269(24):16631-7.
Null thioredoxin and glutaredoxin Escherichia coli K-12 mutants have no enhanced sensitivity to mutagens due to a new GSH-dependent hydrogen donor and high increases in ribonucleotide reductase activity.
Miranda-Vizuete A, Martinez-Galisteo E, Aslund F, Lopez-Barea J, Pueyo C, Holmgren A.
(
8206982
)
|
|
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Deletion
|
OCR02-11
(D)
,
trxA307(del)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
trxA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
trxA
Search strains by all related name:
trxA b3781 dasC ECK3773 fip fipA JW5856 o127 tsnC
|
General information (Go to Linear View: )
|
|
Gene Name
|
trxA
|
|
Alternative name
|
b3781,dasC,ECK3773,fip,fipA,JW5856,o127,tsnC
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Location, Length
|
3,963,784 - 3,964,113
(
+
)
;
85.43
min
;
330
(bp) ,
109
(aa)
|
|
Product
|
thioredoxin 1
|
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Operon Name
|
trxA
|
|
Note
|
|
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Function
|
enzyme; Biosynthesis of cofactors, carriers: Thioredoxin, glutaredoxin, glutathione
|
|
Gene Ontology
|
GO:0006118
;
electron transport ( trxA )
GO:0006662
;
glycerol ether metabolic process ( trxA )
GO:0006810
;
transport ( trxA )
GO:0009055
;
electron carrier activity ( trxA )
GO:0015035
;
protein disulfide oxidoreductase activity ( trxA )
GO:0045454
;
cell redox homeostasis ( trxA )
|
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PID
|
87082331
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
trxA
(
EG11031
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
THIO_ECOLI
(
P00274
;
P76750; Q47674; Q8XAT2
)
|
|
- Protein name
|
Thioredoxin 1
|
|
- Synonyms
|
TRX1, TRX
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|
- Gene name
|
t3381, SF3854, S3905; ORFNames=STMD1.75;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
trxA
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Thioredoxin Mutant P34h
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Thioredoxin 1 (TRX1) (TRX)
|
|
PSI-BLAST
|
Thioredoxin 1 (TRX1) (TRX)
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
thioredoxin/transketolase fusion protein [synthetic construct]
|
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Pfam 28.0
(database updated :
2015-05
)
|
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Pfam
|
|
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PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
THIOREDOXIN
|
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MMBR References
|
Mol Gen Genet. 1988;213(2-3):471-8.
Suppression of the Escherichia coli dnaA46 mutation by a mutation in trxA, the gene for thioredoxin.
Hupp TR, Kaguni JM.
(
3054487
)
|
J Bacteriol. 1985;161(2):799-802.
Thioredoxin is the bacterial protein encoded by fip that is required for filamentous bacteriophage f1 assembly.
Lim CJ, Haller B, Fuchs JA.
(
3881414
)
|
J Bacteriol. 1985;163(1):311-6.
Cloning and nucleotide sequence of the trxA gene of Escherichia coli K-12.
Lim CJ, Geraghty D, Fuchs JA.
(
3891733
)
|
Mol Gen Genet. 1977;155(2):145-52.
Genetic mapping of trxA, a gene affecting thioredoxin in Escherichia coli K12.
Mark DF, Chase JW, Richardson CC.
(
337124
)
|
J Bacteriol. 1986;166(3):945-58.
Autogenous regulation of the gene for transcription termination factor rho in Escherichia coli: localization and function of its attenuators.
Matsumoto Y, Shigesada K, Hirano M, Imai M.
(
2423505
)
|
J Bacteriol. 1983;154(3):1064-76.
A bacterial gene, fip, required for filamentous bacteriophage fl assembly.
Russel M, Model P.
(
6343342
)
|
J Bacteriol. 1984;157(2):526-32.
Characterization of the cloned fip gene and its product.
Russel M, Model P.
(
6319363
)
|
Proc Natl Acad Sci U S A. 1985;82:29-33
Thioredoxin is required for filamentous phage assembly.
Russel, M., and P. Model.
|
J Bacteriol. 1990;172(4):1923-9.
Thioredoxin or glutaredoxin in Escherichia coli is essential for sulfate reduction but not for deoxyribonucleotide synthesis.
Russel M, Model P, Holmgren A.
(
2180911
)
|
Biochem Biophys Res Commun. 1997;234(3):564-7.
Expression of the Escherichia coli thioredoxin gene is negatively regulated by cyclic AMP.
Sa JH, Namgung MA, Lim CJ, Fuchs JA.
(
9175752
)
|
Nucleic Acids Res. 1988;16:10803-10816
The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping.
Sands, J. F., P. Regnier, H. S. Cummings, M. Grunberg-Manago, and J. W. Hershey.
|
Gene. 1984;32(3):399-408.
Genetic and physical analysis of the thioredoxin (trxA) gene of Escherichia coli K-12.
Wallace BJ, Kushner SR.
(
6099324
)
|
J Mol Biol. 1988;200(3):427-38.
Generation of a detailed physical and genetic map of the ilv-metE-udp region of the Escherichia coli chromosome.
Aldea M, Maples VF, Kushner SR.
(
3294419
)
|
Science. 1992;257(5071):771-8.
Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes.
Daniels DL, Plunkett G 3rd, Burland VD, Blattner FR.
(
1379743
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK
0071 YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLA
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TTACGAAAGC GTATCCGGTG AAATAAAGTC
-070 AACCTTTAGT TGGTTAATGT TACACCAACA ACGAAACCAA CACGCCAGGC TTATTCCTGT GGAGTTATAT
0001 atgagcgata aaattattca cctgactgac gacagttttg acacggatgt actcaaagcg gacggggcga
0071 tcctcgtcga tttctgggca gagtggtgcg gtccgtgcaa aatgatcgcc ccgattctgg atgaaatcgc
0141 tgacgaatat cagggcaaac tgaccgttgc aaaactgaac atcgatcaaa accctggcac tgcgccgaaa
0211 tatggcatcc gtggtatccc gactctgctg ctgttcaaaa acggtgaagt ggcggcaacc aaagtgggtg
0281 cactgtctaa aggtcagttg aaagagttcc tcgacgctaa cctggcgtaa GGGAATTTCA TGTTCGGGTG
0351 CCCCGTCGCT AAAAACTGGA CGCCCGGCGT GAGTCATGCT AACTTAGTGT TGACTTCGTA TTAAACATAC
0421 CTTATTAAGT
|
