MG1655
W3110
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Gene Report : glmS
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1989;171(12):6586-92.
Alternative route for biosynthesis of amino sugars in Escherichia coli K-12 mutants by means of a catabolic isomerase.
Vogler AP, Trentmann S, Lengeler JW. ( 2687246 )
     Deletion OCL01 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  glmS
Related strains
     Strains Search Search strains by gene name:  glmS   Search strains by all related name:  glmS b3729 ECK3722 f609 JW3707

General information  (Go to Linear View:)
 Gene Name glmS  
 Alternative name b3729,ECK3722,f609,JW3707  
 Location, Length 3,909,862 - 3,911,691 (  -  ) ;   84.27 min ; 1830 (bp) ,   609 (aa) Go to Linear View
 Product L-glutamine:D-fructose-6-phosphate aminotransferase  
 Operon Name glmUS  
 Note GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009243 - O antigen biosynthetic process; GO_process: GO:0009252 - peptidoglycan biosynthetic process; GO_process: GO:0046349 - amino sugar biosynthetic process  
 Function enzyme; Central intermediary metabolism: Amino sugars  
 Gene Ontology GO:0004360; glutamine-fructose-6-phosphate transaminase (isomerizing) activity ( glmS )
GO:0005515; protein binding ( glmS )
GO:0005529; sugar binding ( glmS )
GO:0005737; cytoplasm ( glmS )
GO:0005975; carbohydrate metabolic process ( glmS )
GO:0006541; glutamine metabolic process ( glmS )
GO:0008152; metabolic process ( glmS )
GO:0008483; transaminase activity ( glmS )
GO:0016051; carbohydrate biosynthetic process ( glmS )
GO:0016740; transferase activity ( glmS )
 PID 1790167  
 EC number
  (KEGG Pathway)
 
2.6.1.16  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene glmS ( EG10382 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) GLMS_ECOLI ( P17169 ; P76745 )
    -  Protein name Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]  
    -  Synonyms EC 2.6.1.16, Hexosephosphate aminotransferase, D-fructose-6- phosphate amidotransferase, GFAT, L-glutamine-D-fructose-6-phosphate amidotransferase, Glucosamine-6-phosphate synthase  
    -  Gene name Name=glmS; OrderedLocusNames=b3729;  

 Linear View (Whole Mode)
View Location
   3900.0  –  3925.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP glmS (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Glucosamine 6-Phosphate Synthase With Glucose 6-Phosphate  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]  
         PSI-BLAST Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]
    nr     (database updated : 2007.02.20 )
         BLAST D-fructose-6-phosphate amidotransferase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    RGD    GATASE_TYPE_II     

Other Cross-Reference
    COG COG0449M 
    EcoCyc glmS 

MMBR References
J Bacteriol. 1993;175:4951-4956
Coordinated regulation of amino sugar-synthesizing and -degrading enzymes in Escherichia coli K-12.
Plumbridge, J. A., O. Cochet, J. M. Souza, M. M. Altamirano, and M. L. Calcagno.
ICN-UCLA Symp. Mol. Cell. Biol. 1980;19:137-159.
The origin of replication, oriC, of the Escherichia coli chromosome: genes near oriC and construction of oriC deletion mutations.
von Meyenburg, K., F. G. Hansen, E. Riise, H. E. N. Bergmans, M. Meijer, and W. Messer.
Biochem J. 1984;224(3):799-815.
DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS.
Walker JE, Gay NJ, Saraste M, Eberle AN. ( 6395859 )

Sequences
Amino acid
FASTA format
0001 MCGIVGAIAQ RDVAEILLEG LRRLEYRGYD SAGLAVVDAE GHMTRLRRLG KVQMLAQAAE EHPLHGGTGI 
0071 AHTRWATHGE PSEVNAHPHV SEHIVVVHNG IIENHEPLRE ELKARGYTFV SETDTEVIAH LVNWELKQGG 
0141 TLREAVLRAI PQLRGAYGTV IMDSRHPDTL LAARSGSPLV IGLGMGENFI ASDQLALLPV TRRFIFLEEG 
0211 DIAEITRRSV NIFDKTGAEV KRQDIESNLQ YDAGDKGIYR HYMQKEIYEQ PNAIKNTLTG RISHGQVDLS 
0281 ELGPNADELL SKVEHIQILA CGTSYNSGMV SRYWFESLAG IPCDVEIASE FRYRKSAVRR NSLMITLSQS 
0351 GETADTLAGL RLSKELGYLG SLAICNVPGS SLVRESDLAL MTNAGTEIGV ASTKAFTTQL TVLLMLVAKL 
0421 SRLKGLDASI EHDIVHGLQA LPSRIEQMLS QDKRIEALAE DFSDKHHALF LGRGDQYPIA LEGALKLKEI 
0491 SYIHAEAYAA GELKHGPLAL IDADMPVIVV APNNELLEKL KSNIEEVRAR GGQLYVFADQ DAGFVSSDNM 
0561 HIIEMPHVEE VIAPIFYTVP LQLLAYHVAL IKGTDVDQPR NLAKSVTVE

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             agtaactata aaaataaccc cactctctac 
-070 aaggctcggg gcgcccgaaa aaacgggcat acaggttgac cgacaacgat ataaatcgga atcaaaaact 
0001 atgtgtggaa ttgttggcgc gatcgcgcaa cgtgatgtag cagaaatcct tcttgaaggt ttacgtcgtc 
0071 tggaataccg cggatatgac tctgccggtc tggccgttgt tgatgcagaa ggtcatatga cccgcctgcg 
0141 tcgcctcggt aaagtccaga tgctggcaca ggcagcggaa gaacatcctc tgcatggcgg cactggtatt 
0211 gctcacactc gctgggcgac ccacggtgaa ccttcagaag tgaatgcgca tccgcatgtt tctgaacaca 
0281 ttgtggtggt gcataacggc atcatcgaaa accatgaacc gctgcgtgaa gagctaaaag cgcgtggcta 
0351 taccttcgtt tctgaaaccg acaccgaagt gattgcccat ctggtgaact gggagctgaa acaaggcggg 
0421 actctgcgtg aggccgttct gcgtgctatc ccgcagctgc gtggtgcgta cggtacagtg atcatggact 
0491 cccgtcaccc ggataccctg ctggcggcac gttctggtag tccgctggtg attggcctgg ggatgggcga 
0561 aaactttatc gcttctgacc agctggcgct gttgccggtg acccgtcgct ttatcttcct tgaagagggc 
0631 gatattgcgg aaatcactcg ccgttcggta aacatcttcg ataaaactgg cgcggaagta aaacgtcagg 
0701 atatcgaatc caatctgcaa tatgacgcgg gcgataaagg catttaccgt cactacatgc agaaagagat 
0771 ctacgaacag ccgaacgcga tcaaaaacac ccttaccgga cgcatcagcc acggtcaggt tgatttaagc 
0841 gagctgggac cgaacgccga cgaactgctg tcgaaggttg agcatattca gatcctcgcc tgtggtactt 
0911 cttataactc cggtatggtt tcccgctact ggtttgaatc gctagcaggt attccgtgcg acgtcgaaat 
0981 cgcctctgaa ttccgctatc gcaaatctgc cgtgcgtcgt aacagcctga tgatcacctt gtcacagtct 
1051 ggcgaaaccg cggataccct ggctggcctg cgtctgtcga aagagctggg ttaccttggt tcactggcaa 
1121 tctgtaacgt tccgggttct tctctggtgc gcgaatccga tctggcgcta atgaccaacg cgggtacaga 
1191 aatcggcgtg gcatccacta aagcattcac cactcagtta actgtgctgt tgatgctggt ggcgaagctg 
1261 tctcgcctga aaggtctgga tgcctccatt gaacatgaca tcgtgcatgg tctgcaggcg ctgccgagcc 
1331 gtattgagca gatgctgtct caggacaaac gcattgaagc gctggcagaa gatttctctg acaaacatca 
1401 cgcgctgttc ctgggccgtg gcgatcagta cccaatcgcg ctggaaggcg cattgaagtt gaaagagatc 
1471 tcttacattc acgctgaagc ctacgctgct ggcgaactga aacacggtcc gctggcgcta attgatgccg 
1541 atatgccggt tattgttgtt gcaccgaaca acgaattgct ggaaaaactg aaatccaaca ttgaagaagt 
1611 tcgcgcgcgt ggcggtcagt tgtatgtctt cgccgatcag gatgcgggtt ttgtaagtag cgataacatg 
1681 cacatcatcg agatgccgca tgtggaagag gtgattgcac cgatcttcta caccgttccg ctgcagctgc 
1751 tggcttacca tgtcgcgctg atcaaaggca ccgacgttga ccagccgcgt aacctggcaa aatcggttac 
1821 ggttgagtaa taaatggatg ccctgcgtaa gcggggcatt tttcttcctg ttatgttttt aatcaaacat 
1891 cctgccaact ccatgtgaca aaccgtcatc ttcggctact