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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Deletion
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OCL03
(D)
,
DE(OC2)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
tnaA
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Related strains
|
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Strains Search
|
Search strains by gene name:
tnaA
Search strains by all related name:
tnaA b3708 ECK3701 ind JW3686 o476 tnaR
|
General information (Go to Linear View: )
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Gene Name
|
tnaA
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Alternative name
|
b3708,ECK3701,ind,JW3686,o476,tnaR
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Location, Length
|
3,886,753 - 3,888,168
(
+
)
;
83.77
min
;
1416
(bp) ,
471
(aa)
|
|
Product
|
tryptophanase/L-cysteine desulfhydrase, PLP-dependent
|
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Operon Name
|
tnaCAB
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Note
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tryptophanase; GO_process: GO:0009063 - cellular amino acid catabolic process
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Function
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enzyme; Degradation of small molecules: Amino acids
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Gene Ontology
|
GO:0003824
;
catalytic activity ( tnaA )
GO:0005515
;
protein binding ( tnaA )
GO:0005624
;
membrane fraction ( tnaA )
GO:0006520
;
amino acid metabolic process ( tnaA )
GO:0006568
;
tryptophan metabolic process ( tnaA )
GO:0006569
;
tryptophan catabolic process ( tnaA )
GO:0009034
;
tryptophanase activity ( tnaA )
GO:0009072
;
aromatic amino acid family metabolic process ( tnaA )
GO:0016829
;
lyase activity ( tnaA )
GO:0016830
;
carbon-carbon lyase activity ( tnaA )
GO:0030170
;
pyridoxal phosphate binding ( tnaA )
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|
PID
|
87082323
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EC number
(KEGG Pathway)
|
4.1.99.1
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
tnaA
(
EG11005
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
TNAA_ECOLI
(
P00913
;
P78123
)
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- Protein name
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Tryptophanase
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- Synonyms
|
EC 4.1.99.1, L-tryptophan indole-lyase, TNase
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- Gene name
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Name=tnaA; Synonyms=ind; OrderedLocusNames=b3708, SF3754, S4017;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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tnaA
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
|
Chain A, Crystal Structure Of E. Coli Tryptophanase
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Tryptophanase (L-tryptophan indole-lyase) (TNase)
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PSI-BLAST
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Glutamate-1-semialdehyde 2,1-aminomutase
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nr
(database updated :
2007.02.20
)
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BLAST
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tryptophanase [Escherichia coli UTI89]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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GLYCOSAMINOGLYCAN
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
RGD
BETA_ELIM_LYASE
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MMBR References
|
J Bacteriol. 1979;140(3):817-24.
Cloning and physical mapping of the dnaA region of the Escherichia coli chromosome.
Miki T, Kimura M, Hiraga S, Nagata T, Yura T.
(
160412
)
|
J Bacteriol. 1965;89:355-64.
FORMATION AND INTERRELATIONSHIPS OF TRYPTOPHANASE AND TRYPTOPHAN SYNTHETASES IN ESCHERICHIA COLI.
Newton WA, Snell EE.
(
14255701
)
|
J Bacteriol. 1963;85:245-6.
Selection of mutants of Escherichia coli constitutive for tryptophanase.
Ng H, Gartner TK.
(
13938299
)
|
J Bacteriol. 1986;166(1):217-23.
Rho-dependent transcription termination in the tryptophanase operon leader region of Escherichia coli K-12.
Stewart V, Landick R, Yanofsky C.
(
2420781
)
|
ICN-UCLA Symp. Mol. Cell. Biol. 1980;19:137-159.
The origin of replication, oriC, of the Escherichia coli chromosome: genes near oriC and construction of oriC deletion mutations.
von Meyenburg, K., F. G. Hansen, E. Riise, H. E. N. Bergmans, M. Meijer, and W. Messer.
|
Proc Natl Acad Sci U S A. 1972;69:1086-1090
Reversibility of the tryptophanase reaction: synthesis of tryptophan from indole, pyruvate, and ammonia.
Watanabe, T., and E. E. Snell.
|
J Bacteriol. 1977;130(1):57-61.
Unstable mutations that relieve catabolite repression of tryptophanase synthesis by Escherichia coli.
Yudkin MD.
(
323244
)
|
Genomics. 1993;16(3):551-61.
DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.
Burland V, Plunkett G 3rd, Daniels DL, Blattner FR.
(
7686882
)
|
J Bacteriol. 1981;147(3):787-96.
Nucleotide sequence of the structural gene for tryptophanase of Escherichia coli K-12.
Deeley MC, Yanofsky C.
(
6268608
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MENFKHLPEP FRIRVIEPVK RTTRAYREEA IIKSGMNPFL LDSEDVFIDL LTDSGTGAVT QSMQAAMMRG
0071 DEAYSGSRSY YALAESVKNI FGYQYTIPTH QGRGAEQIYI PVLIKKREQE KGLDRSKMVA FSNYFFDTTQ
0141 GHSQINGCTV RNVYIKEAFD TGVRYDFKGN FDLEGLERGI EEVGPNNVPY IVATITSNSA GGQPVSLANL
0211 KAMYSIAKKY DIPVVMDSAR FAENAYFIKQ REAEYKDWTI EQITRETYKY ADMLAMSAKK DAMVPMGGLL
0281 CMKDDSFFDV YTECRTLCVV QEGFPTYGGL EGGAMERLAV GLYDGMNLDW LAYRIAQVQY LVDGLEEIGV
0351 VCQQAGGHAA FVDAGKLLPH IPADQFPAQA LACELYKVAG IRAVEIGSFL LGRDPKTGKQ LPCPAELLRL
0421 TIPRATYTQT HMDFIIEAFK HVKENAANIK GLTFTYEPKV LRHFTAKLKE V
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 TAAATGATGG TGCTTGCATA TATATCTGGC
-070 GAATTAATCG GTATAGCAGA TGTAATATTC ACAGGGATCA CTGTAATTAA AATAAATGAA GGATTATGTA
0001 atggaaaact ttaaacatct ccctgaaccg ttccgcattc gtgttattga gccagtaaaa cgtaccactc
0071 gcgcttatcg tgaagaggca attattaaat ccggtatgaa cccgttcctg ctggatagcg aagatgtttt
0141 tatcgattta ctgaccgaca gcggcaccgg ggcggtgacg cagagcatgc aggctgcgat gatgcgcggc
0211 gacgaagcct acagcggcag tcgtagctac tatgcgttag ccgagtcagt gaaaaatatc tttggttatc
0281 aatacaccat tccgactcac cagggccgtg gcgcagagca aatctatatt ccggtactga ttaaaaaacg
0351 cgagcaggaa aaaggcctgg atcgcagcaa aatggtggcg ttctctaact atttctttga taccacgcag
0421 ggccatagcc agatcaacgg ctgtaccgtg cgtaacgtct atatcaaaga agccttcgat acgggcgtgc
0491 gttacgactt taaaggcaac tttgaccttg agggattaga acgcggtatt gaagaagttg gtccgaataa
0561 cgtgccgtat atcgttgcaa ccatcaccag taactctgca ggtggtcagc cggtttcact ggcaaactta
0631 aaagcgatgt acagcatcgc gaagaaatac gatattccgg tggtaatgga ctccgcgcgc tttgctgaaa
0701 acgcctattt catcaagcag cgtgaagcag aatacaaaga ctggaccatc gagcagatca cccgcgaaac
0771 ctacaaatat gccgatatgc tggcgatgtc cgccaagaaa gatgcgatgg tgccgatggg cggcctgctg
0841 tgcatgaaag acgacagctt ctttgatgtg tacaccgagt gcagaaccct ttgcgtggtg caggaaggct
0911 tcccgacata tggcggcctg gaaggcggcg cgatggagcg tctggcggta ggtctgtatg acggcatgaa
0981 tctcgactgg ctggcttatc gtatcgcgca ggtacagtat ctggtcgatg gtctggaaga gattggcgtt
1051 gtctgccagc aggcgggcgg tcacgcggca ttcgttgatg ccggtaaact gttgccgcat atcccggcag
1121 accagttccc ggcacaggcg ctggcctgcg agctgtataa agtcgccggt atccgtgcgg tagaaattgg
1191 ctctttcctg ttaggccgcg atccgaaaac cggtaaacaa ctgccatgcc cggctgaact gctgcgttta
1261 accattccgc gcgcaacata tactcaaaca catatggact tcattattga agcctttaaa catgtgaaag
1331 agaacgcggc gaatattaaa ggattaacct ttacgtacga accgaaagta ttgcgtcact tcaccgcaaa
1401 acttaaagaa gtttaaTTAA TACTACAGAG TGGCTATAAG GATGTTAGCC ACTCTCTTAC CCTACATCCT
1471 CAATAACAAA AATAGCCTTC CTCTAAAGGT GGCATCATGA CTGATC
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