MG1655
W3110
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Gene Report : dnaA
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Mol Biol. 1970;53(3):369-87.
On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation.
Hirota Y, Mordoh J, Jacob F. ( 4924005 )
     Deletion OCL04 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  dnaA
Related strains
     Strains Search Search strains by gene name:  dnaA   Search strains by all related name:  dnaA b3702 ECK3694 hsm-2 JW3679

General information  (Go to Linear View:)
 Gene Name dnaA  
 Alternative name b3702,ECK3694,hsm-2,JW3679  
 Location, Length 3,880,349 - 3,881,752 (  -  ) ;   83.63 min ; 1404 (bp) ,   467 (aa) Go to Linear View
 Product chromosomal replication initiator protein DnaA, DNA-binding transcriptional dual regulator  
 Operon Name dnaAN-recF  
 Note DNA biosynthesis; initiation of chromosome replication; can be transcription regulator; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication  
 Function putative regulator; DNA - replication, repair,restriction/modification  
 Gene Ontology GO:0000166; nucleotide binding ( dnaA )
GO:0003677; DNA binding ( dnaA )
GO:0003688; DNA replication origin binding ( dnaA )
GO:0005524; ATP binding ( dnaA )
GO:0005737; cytoplasm ( dnaA )
GO:0006260; DNA replication ( dnaA )
GO:0006270; DNA replication initiation ( dnaA )
GO:0006275; regulation of DNA replication ( dnaA )
GO:0017111; nucleoside-triphosphatase activity ( dnaA )
GO:0043565; sequence-specific DNA binding ( dnaA )
 PID 2367267  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) DNAA_ECOLI ( P03004 ; P78122 )
    -  Protein name Chromosomal replication initiator protein dnaA.  
    -  Synonyms  
    -  Gene name Name=dnaA; OrderedLocusNames=b3702;  

 Linear View (Whole Mode)
View Location
   3870.0  –  3895.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP dnaA (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Structure Of Amppcp-Bound Dnaa From Aquifex Aeolicus  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Chromosomal replication initiator protein dnaA  
         PSI-BLAST Chromosomal replication initiator protein dnaA
    nr     (database updated : 2007.02.20 )
         BLAST DnaA [Shigella dysenteriae Sd197]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    ATP_GTP_A    DNAA     

Other Cross-Reference
    COG COG0593L 
    EcoCyc dnaA 

MMBR References
J Biol Chem. 1998;273:5167-5173
Membrane-mediated release of nucleotide from an initiator of chromosomal replication, Escherichia coli DnaA, occurs with insertion of a distinct region of the protein into the lipid bilayer.
Garner, J., P. Durrer, J. Kitchen, J. Brunner, and E. Crooke.
Nucleic Acids Res. 1982;10:7373-7385
The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
Hansen, E. B., F. G. Hansen, and K. von Meyenburg.
EMBO J. 1982;1(9):1043-8.
The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli.
Hansen FG, Hansen EB, Atlung T. ( 6329723 )
Mol Gen Genet. 1992;233(1-2):81-8.
Mutations in the DnaA binding sites of the replication origin of Escherichia coli.
Holz A, Schaefer C, Gille H, Jueterbock WR, Messer W. ( 1603077 )
J Biol Chem. 1992;267:23083-23086
Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF.
Hwang, D. S., and A. Kornberg.
J Biol Chem. 1992;267:23087-23091
Opposed actions of regulatory proteins, DnaA and IciA, in opening the replication origin of Escherichia coli.
Hwang, D. S., and A. Kornberg.
J Biol Chem. 1990;265:19244-19248
Aggregated dnaA protein is dissociated and activated for DNA replication by phospholipase or dnaK protein.
Hwang, D. S., E. Crooke, and A. Kornberg.
J Bacteriol. 1996;178:5790-5792
The nucleoid protein H-NS facilitates chromosome DNA replication in Escherichia coli dnaA mutants.
Katayama, T., M. Takata, and K. Sekimizu.
J Bacteriol. 1979;140(3):825-34.
Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication.
Kimura M, Miki T, Hiraga S, Nagata T, Yura T. ( 160413 )
J Bacteriol. 1986;168(1):440-3.
Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance.
Kline BC, Kogoma T, Tam JE, Shields MS. ( 3020005 )
Nucleic Acids Res. 1988;16:2601-2612
Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
Knott, V., D. J. G. Rees, Z. Cheng, and G. G. Brownlee.
Mol Microbiol. 1996;19(2):389-96.
Transcriptional activation of the dnaA gene encoding the initiator for oriC replication by IciA protein, an inhibitor of in vitro oriC replication in Escherichia coli.
Lee YS, Kim H, Hwang DS. ( 8825783 )
J Bacteriol. 1979;140(3):817-24.
Cloning and physical mapping of the dnaA region of the Escherichia coli chromosome.
Miki T, Kimura M, Hiraga S, Nagata T, Yura T. ( 160412 )
J Biol Chem. 1996;271:25178-25183
Molecular design of inhibitors of in vitro oriC DNA replication based on the potential to block the ATP binding of DnaA protein.
Mizushima, T., S. Sasaki, H. Ohishi, M. Kobayashi, T. Katayama, T. Miki, M. Maeda, and K. Sekimizu.
Biochemistry. 1996;35(35):11512-6.
Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli.
Mizushima T, Katayama T, Sekimizu K. ( 8784207 )
Mol Gen Genet. 1980;180(2):235-47.
Characterization of dnaA gene carried by lambda transducing phage.
Murakami A, Inokuchi H, Hirota Y, Ozeki H, Yamagishi H. ( 6450869 )
Gene. 1984;28(2):159-70.
Structural analysis of the dnaA and dnaN genes of Escherichia coli.
Ohmori H, Kimura M, Nagata T, Sakakibara Y. ( 6234204 )
J Bacteriol. 1995;177:1896-1899
Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box.
Perez-Roger, I., F. Macian, and M.-E. Armengod.
J Biol Chem. 1992;267:9140-9145
Transcriptional and maturational effects of manganese and iron on the biosynthesis of manganese-superoxide dismutase in Escherichia coli.
Privalle, C. T., and I. Fridovich.
Mol Gen Genet. 1988;213(1):118-24.
Discoordinate gene expression in the dnaA-dnaN operon of Escherichia coli.
Quinones A, Messer W. ( 2851700 )
Mol Gen Genet. 1991;227(1):9-16.
Expression of the dnaA gene of Escherichia coli is inducible by DNA damage.
Quinones A, Juterbock WR, Messer W. ( 1904539 )
Gene. 1981;13(1):47-55.
Organization and transcription of the dnaA and dnaN genes of Escherichia coli.
Sakakibara Y, Tsukano H, Sako T. ( 6453739 )
Mol Gen Genet. 1980;179(3):521-6.
Coordinate expression of Escherichia coli dnaA and dnaN genes.
Sako T, Sakakibara Y. ( 6449652 )
J Bacteriol. 1988;170(2):852-8.
Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles.
Skarstad K, von Meyenburg K, Hansen FG, Boye E. ( 2828328 )
J Bacteriol. 1995;177:6657-6665
Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.
Sutton, M. D., and J. M. Kaguni.
J Mol Biol. 1997;271(5):693-703.
Novel alleles of the Escherichia coli dnaA gene.
Sutton MD, Kaguni JM. ( 9299320 )
ICN-UCLA Symp. Mol. Cell. Biol. 1980;19:137-159.
The origin of replication, oriC, of the Escherichia coli chromosome: genes near oriC and construction of oriC deletion mutations.
von Meyenburg, K., F. G. Hansen, E. Riise, H. E. N. Bergmans, M. Meijer, and W. Messer.
Proc Natl Acad Sci U S A. 1996;93:12206-12211
Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins.
Boye, E., T. Stokke, N. Kleckner, and K. Skarstad.
Mol Gen Genet. 1980;180(2):267-73.
Identification of the dnaA and dnaN gene products of Escherichia coli.
Yuasa S, Sakakibara Y. ( 6258023 )

Sequences
Amino acid
FASTA format
0001 MSLSLWQQCL ARLQDELPAT EFSMWIRPLQ AELSDNTLAL YAPNRFVLDW VRDKYLNNIN GLLTSFCGAD 
0071 APQLRFEVGT KPVTQTPQAA VTSNVAAPAQ VAQTQPQRAA PSTRSGWDNV PAPAEPTYRS NVNVKHTFDN 
0141 FVEGKSNQLA RAAARQVADN PGGAYNPLFL YGGTGLGKTH LLHAVGNGIM ARKPNAKVVY MHSERFVQDM 
0211 VKALQNNAIE EFKRYYRSVD ALLIDDIQFF ANKERSQEEF FHTFNALLEG NQQIILTSDR YPKEINGVED 
0281 RLKSRFGWGL TVAIEPPELE TRVAILMKKA DENDIRLPGE VAFFIAKRLR SNVRELEGAL NRVIANANFT 
0351 GRAITIDFVR EALRDLLALQ EKLVTIDNIQ KTVAEYYKIK VADLLSKRRS RSVARPRQMA MALAKELTNH 
0421 SLPEIGDAFG GRDHTTVLHA CRKIEQLREE SHDIKEDFSN LIRTLSS

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             ttcgtgcgtc accctcaagc agggtctttt 
-070 cgacgtacgt caacaatcat gaatgtttca gccttagtca ttatcgactt ttgttcgagt ggagtccgcc 
0001 gtgtcacttt cgctttggca gcagtgtctt gcccgattgc aggatgagtt accagccaca gaattcagta 
0071 tgtggatacg cccattgcag gcggaactga gcgataacac gctggccctg tacgcgccaa accgttttgt 
0141 cctcgattgg gtacgggaca agtaccttaa taatatcaat ggactgctaa ccagtttctg cggagcggat 
0211 gccccacagc tgcgttttga agtcggcacc aaaccggtga cgcaaacgcc acaagcggca gtgacgagca 
0281 acgtcgcggc ccctgcacag gtggcgcaaa cgcagccgca acgtgctgcg ccttctacgc gctcaggttg 
0351 ggataacgtc ccggccccgg cagaaccgac ctatcgttct aacgtaaacg tcaaacacac gtttgataac 
0421 ttcgttgaag gtaaatctaa ccaactggcg cgcgcggcgg ctcgccaggt ggcggataac cctggcggtg 
0491 cctataaccc gttgttcctt tatggcggca cgggtctggg taaaactcac ctgctgcatg cggtgggtaa 
0561 cggcattatg gcgcgcaagc cgaatgccaa agtggtttat atgcactccg agcgctttgt tcaggacatg 
0631 gttaaagccc tgcaaaacaa cgcgatcgaa gagtttaaac gctactaccg ttccgtagat gcactgctga 
0701 tcgacgatat tcagtttttt gctaataaag aacgatctca ggaagagttt ttccacacct tcaacgccct 
0771 gctggaaggt aatcaacaga tcattctcac ctcggatcgc tatccgaaag agatcaacgg cgttgaggat 
0841 cgtttgaaat cccgcttcgg ttggggactg actgtggcga tcgaaccgcc agagctggaa acccgtgtgg 
0911 cgatcctgat gaaaaaggcc gacgaaaacg acattcgttt gccgggcgaa gtggcgttct ttatcgccaa 
0981 gcgtctacga tctaacgtac gtgagctgga aggggcgctg aaccgcgtca ttgccaatgc caactttacc 
1051 ggacgggcga tcaccatcga cttcgtgcgt gaggcgctgc gcgacttgct ggcattgcag gaaaaactgg 
1121 tcaccatcga caatattcag aagacggtgg cggagtacta caagatcaaa gtcgcggatc tcctttccaa 
1191 gcgtcgatcc cgctcggtgg cgcgtccgcg ccagatggcg atggcgctgg cgaaagagct gactaaccac 
1261 agtctgccgg agattggcga tgcgtttggt ggccgtgacc acacgacggt gcttcatgcc tgccgtaaga 
1331 tcgagcagtt gcgtgaagag agccacgata tcaaagaaga tttttcaaat ttaatcagaa cattgtcatc 
1401 gtaaacctat gaaatttacc gtagaacgtg agcatttatt aaaaccgcta caacaggtga gcggtccgtt 
1471 aggtggtcgt cctacgctac cgattctcgg taat