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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Mol Biol. 1970;53(3):369-87.
On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation.
Hirota Y, Mordoh J, Jacob F.
(
4924005
)
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Deletion
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OCL04
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
dnaA
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Related strains
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Strains Search
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Search strains by gene name:
dnaA
Search strains by all related name:
dnaA b3702 ECK3694 hsm-2 JW3679
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General information (Go to Linear View: )
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Gene Name
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dnaA
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Alternative name
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b3702,ECK3694,hsm-2,JW3679
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Location, Length
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3,880,349 - 3,881,752
(
-
)
;
83.63
min
;
1404
(bp) ,
467
(aa)
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Product
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chromosomal replication initiator protein DnaA, DNA-binding transcriptional dual regulator
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Operon Name
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dnaAN-recF
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Note
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DNA biosynthesis; initiation of chromosome replication; can be transcription regulator; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication
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Function
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putative regulator; DNA - replication, repair,restriction/modification
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Gene Ontology
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GO:0000166
;
nucleotide binding ( dnaA )
GO:0003677
;
DNA binding ( dnaA )
GO:0003688
;
DNA replication origin binding ( dnaA )
GO:0005524
;
ATP binding ( dnaA )
GO:0005737
;
cytoplasm ( dnaA )
GO:0006260
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DNA replication ( dnaA )
GO:0006270
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DNA replication initiation ( dnaA )
GO:0006275
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regulation of DNA replication ( dnaA )
GO:0017111
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nucleoside-triphosphatase activity ( dnaA )
GO:0043565
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sequence-specific DNA binding ( dnaA )
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PID
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2367267
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EC number
(KEGG Pathway)
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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DNAA_ECOLI
(
P03004
;
P78122
)
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- Protein name
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Chromosomal replication initiator protein dnaA.
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- Synonyms
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- Gene name
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Name=dnaA; OrderedLocusNames=b3702;
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MMBR References
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J Biol Chem. 1998;273:5167-5173
Membrane-mediated release of nucleotide from an initiator of chromosomal replication, Escherichia coli DnaA, occurs with insertion of a distinct region of the protein into the lipid bilayer.
Garner, J., P. Durrer, J. Kitchen, J. Brunner, and E. Crooke.
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Nucleic Acids Res. 1982;10:7373-7385
The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
Hansen, E. B., F. G. Hansen, and K. von Meyenburg.
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EMBO J. 1982;1(9):1043-8.
The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli.
Hansen FG, Hansen EB, Atlung T.
(
6329723
)
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Mol Gen Genet. 1992;233(1-2):81-8.
Mutations in the DnaA binding sites of the replication origin of Escherichia coli.
Holz A, Schaefer C, Gille H, Jueterbock WR, Messer W.
(
1603077
)
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J Biol Chem. 1992;267:23083-23086
Opening of the replication origin of Escherichia coli by DnaA protein with protein HU or IHF.
Hwang, D. S., and A. Kornberg.
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J Biol Chem. 1992;267:23087-23091
Opposed actions of regulatory proteins, DnaA and IciA, in opening the replication origin of Escherichia coli.
Hwang, D. S., and A. Kornberg.
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J Biol Chem. 1990;265:19244-19248
Aggregated dnaA protein is dissociated and activated for DNA replication by phospholipase or dnaK protein.
Hwang, D. S., E. Crooke, and A. Kornberg.
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J Bacteriol. 1996;178:5790-5792
The nucleoid protein H-NS facilitates chromosome DNA replication in Escherichia coli dnaA mutants.
Katayama, T., M. Takata, and K. Sekimizu.
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J Bacteriol. 1979;140(3):825-34.
Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication.
Kimura M, Miki T, Hiraga S, Nagata T, Yura T.
(
160413
)
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J Bacteriol. 1986;168(1):440-3.
Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance.
Kline BC, Kogoma T, Tam JE, Shields MS.
(
3020005
)
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Nucleic Acids Res. 1988;16:2601-2612
Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.
Knott, V., D. J. G. Rees, Z. Cheng, and G. G. Brownlee.
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Mol Microbiol. 1996;19(2):389-96.
Transcriptional activation of the dnaA gene encoding the initiator for oriC replication by IciA protein, an inhibitor of in vitro oriC replication in Escherichia coli.
Lee YS, Kim H, Hwang DS.
(
8825783
)
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J Bacteriol. 1979;140(3):817-24.
Cloning and physical mapping of the dnaA region of the Escherichia coli chromosome.
Miki T, Kimura M, Hiraga S, Nagata T, Yura T.
(
160412
)
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J Biol Chem. 1996;271:25178-25183
Molecular design of inhibitors of in vitro oriC DNA replication based on the potential to block the ATP binding of DnaA protein.
Mizushima, T., S. Sasaki, H. Ohishi, M. Kobayashi, T. Katayama, T. Miki, M. Maeda, and K. Sekimizu.
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Biochemistry. 1996;35(35):11512-6.
Effect on DNA topology by DnaA protein, the initiation factor of chromosomal DNA replication in Escherichia coli.
Mizushima T, Katayama T, Sekimizu K.
(
8784207
)
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Mol Gen Genet. 1980;180(2):235-47.
Characterization of dnaA gene carried by lambda transducing phage.
Murakami A, Inokuchi H, Hirota Y, Ozeki H, Yamagishi H.
(
6450869
)
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Gene. 1984;28(2):159-70.
Structural analysis of the dnaA and dnaN genes of Escherichia coli.
Ohmori H, Kimura M, Nagata T, Sakakibara Y.
(
6234204
)
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J Bacteriol. 1995;177:1896-1899
Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box.
Perez-Roger, I., F. Macian, and M.-E. Armengod.
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J Biol Chem. 1992;267:9140-9145
Transcriptional and maturational effects of manganese and iron on the biosynthesis of manganese-superoxide dismutase in Escherichia coli.
Privalle, C. T., and I. Fridovich.
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Mol Gen Genet. 1988;213(1):118-24.
Discoordinate gene expression in the dnaA-dnaN operon of Escherichia coli.
Quinones A, Messer W.
(
2851700
)
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Mol Gen Genet. 1991;227(1):9-16.
Expression of the dnaA gene of Escherichia coli is inducible by DNA damage.
Quinones A, Juterbock WR, Messer W.
(
1904539
)
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Gene. 1981;13(1):47-55.
Organization and transcription of the dnaA and dnaN genes of Escherichia coli.
Sakakibara Y, Tsukano H, Sako T.
(
6453739
)
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Mol Gen Genet. 1980;179(3):521-6.
Coordinate expression of Escherichia coli dnaA and dnaN genes.
Sako T, Sakakibara Y.
(
6449652
)
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J Bacteriol. 1988;170(2):852-8.
Coordination of chromosome replication initiation in Escherichia coli: effects of different dnaA alleles.
Skarstad K, von Meyenburg K, Hansen FG, Boye E.
(
2828328
)
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J Bacteriol. 1995;177:6657-6665
Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.
Sutton, M. D., and J. M. Kaguni.
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J Mol Biol. 1997;271(5):693-703.
Novel alleles of the Escherichia coli dnaA gene.
Sutton MD, Kaguni JM.
(
9299320
)
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ICN-UCLA Symp. Mol. Cell. Biol. 1980;19:137-159.
The origin of replication, oriC, of the Escherichia coli chromosome: genes near oriC and construction of oriC deletion mutations.
von Meyenburg, K., F. G. Hansen, E. Riise, H. E. N. Bergmans, M. Meijer, and W. Messer.
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Proc Natl Acad Sci U S A. 1996;93:12206-12211
Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins.
Boye, E., T. Stokke, N. Kleckner, and K. Skarstad.
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Mol Gen Genet. 1980;180(2):267-73.
Identification of the dnaA and dnaN gene products of Escherichia coli.
Yuasa S, Sakakibara Y.
(
6258023
)
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| Sequences |
Amino acid
FASTA format
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0001 MSLSLWQQCL ARLQDELPAT EFSMWIRPLQ AELSDNTLAL YAPNRFVLDW VRDKYLNNIN GLLTSFCGAD
0071 APQLRFEVGT KPVTQTPQAA VTSNVAAPAQ VAQTQPQRAA PSTRSGWDNV PAPAEPTYRS NVNVKHTFDN
0141 FVEGKSNQLA RAAARQVADN PGGAYNPLFL YGGTGLGKTH LLHAVGNGIM ARKPNAKVVY MHSERFVQDM
0211 VKALQNNAIE EFKRYYRSVD ALLIDDIQFF ANKERSQEEF FHTFNALLEG NQQIILTSDR YPKEINGVED
0281 RLKSRFGWGL TVAIEPPELE TRVAILMKKA DENDIRLPGE VAFFIAKRLR SNVRELEGAL NRVIANANFT
0351 GRAITIDFVR EALRDLLALQ EKLVTIDNIQ KTVAEYYKIK VADLLSKRRS RSVARPRQMA MALAKELTNH
0421 SLPEIGDAFG GRDHTTVLHA CRKIEQLREE SHDIKEDFSN LIRTLSS
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 ttcgtgcgtc accctcaagc agggtctttt
-070 cgacgtacgt caacaatcat gaatgtttca gccttagtca ttatcgactt ttgttcgagt ggagtccgcc
0001 gtgtcacttt cgctttggca gcagtgtctt gcccgattgc aggatgagtt accagccaca gaattcagta
0071 tgtggatacg cccattgcag gcggaactga gcgataacac gctggccctg tacgcgccaa accgttttgt
0141 cctcgattgg gtacgggaca agtaccttaa taatatcaat ggactgctaa ccagtttctg cggagcggat
0211 gccccacagc tgcgttttga agtcggcacc aaaccggtga cgcaaacgcc acaagcggca gtgacgagca
0281 acgtcgcggc ccctgcacag gtggcgcaaa cgcagccgca acgtgctgcg ccttctacgc gctcaggttg
0351 ggataacgtc ccggccccgg cagaaccgac ctatcgttct aacgtaaacg tcaaacacac gtttgataac
0421 ttcgttgaag gtaaatctaa ccaactggcg cgcgcggcgg ctcgccaggt ggcggataac cctggcggtg
0491 cctataaccc gttgttcctt tatggcggca cgggtctggg taaaactcac ctgctgcatg cggtgggtaa
0561 cggcattatg gcgcgcaagc cgaatgccaa agtggtttat atgcactccg agcgctttgt tcaggacatg
0631 gttaaagccc tgcaaaacaa cgcgatcgaa gagtttaaac gctactaccg ttccgtagat gcactgctga
0701 tcgacgatat tcagtttttt gctaataaag aacgatctca ggaagagttt ttccacacct tcaacgccct
0771 gctggaaggt aatcaacaga tcattctcac ctcggatcgc tatccgaaag agatcaacgg cgttgaggat
0841 cgtttgaaat cccgcttcgg ttggggactg actgtggcga tcgaaccgcc agagctggaa acccgtgtgg
0911 cgatcctgat gaaaaaggcc gacgaaaacg acattcgttt gccgggcgaa gtggcgttct ttatcgccaa
0981 gcgtctacga tctaacgtac gtgagctgga aggggcgctg aaccgcgtca ttgccaatgc caactttacc
1051 ggacgggcga tcaccatcga cttcgtgcgt gaggcgctgc gcgacttgct ggcattgcag gaaaaactgg
1121 tcaccatcga caatattcag aagacggtgg cggagtacta caagatcaaa gtcgcggatc tcctttccaa
1191 gcgtcgatcc cgctcggtgg cgcgtccgcg ccagatggcg atggcgctgg cgaaagagct gactaaccac
1261 agtctgccgg agattggcga tgcgtttggt ggccgtgacc acacgacggt gcttcatgcc tgccgtaaga
1331 tcgagcagtt gcgtgaagag agccacgata tcaaagaaga tttttcaaat ttaatcagaa cattgtcatc
1401 gtaaacctat gaaatttacc gtagaacgtg agcatttatt aaaaccgcta caacaggtga gcggtccgtt
1471 aggtggtcgt cctacgctac cgattctcgg taat
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