MG1655
W3110
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Gene Report : dnaN
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Mol Gen Genet. 1980;178(3):541-53.
A temperature-sensitive Escherichia coli mutant defective in DNA replication: dnaN, a new gene adjacent to the dnaA gene.
Sakakibara Y, Mizukami T. ( 6248733 )
     Deletion OCL04 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  dnaN
Related strains
     Strains Search Search strains by gene name:  dnaN   Search strains by all related name:  dnaN b3701 ECK3693 f366 JW3678

General information  (Go to Linear View:)
 Gene Name dnaN  
 Alternative name b3701,ECK3693,f366,JW3678  
 Location, Length 3,879,244 - 3,880,344 (  -  ) ;   83.61 min ; 1101 (bp) ,   366 (aa) Go to Linear View
 Product DNA polymerase III, beta subunit  
 Operon Name dnaAN-recF  
 Note DNA polymerase III, beta-subunit; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication  
 Function enzyme; DNA - replication, repair,restriction/modification  
 Gene Ontology GO:0003677; DNA binding ( dnaN )
GO:0003887; DNA-directed DNA polymerase activity ( dnaN )
GO:0005515; protein binding ( dnaN )
GO:0005737; cytoplasm ( dnaN )
GO:0006260; DNA replication ( dnaN )
GO:0008408; 3'-5' exonuclease activity ( dnaN )
GO:0016740; transferase activity ( dnaN )
GO:0016779; nucleotidyltransferase activity ( dnaN )
 PID 1790136  
 EC number
  (KEGG Pathway)
 
2.7.7.7  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene dnaN ( EG10242 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) DP3B_ECOLI ( P00583 )
    -  Protein name DNA polymerase III, beta chain  
    -  Synonyms EC 2.7.7.7  
    -  Gene name Name=dnaN; OrderedLocusNames=b3701, c4623, z5192, ECs4636;  

 Linear View (Whole Mode)
View Location
   3870.0  –  3895.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP dnaN (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Pol Iii (Beta Subunit) (E.C.2.7.7.7)  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST DNA polymerase III subunit beta  
         PSI-BLAST DNA polymerase III subunit beta
    nr     (database updated : 2007.02.20 )
         BLAST DNA polymerase III subunit beta [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0592L 
    EcoCyc dnaN 

MMBR References
Gene. 1986;43(3):183-96.
Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences.
Armengod ME, Lambies E. ( 3527871 )
Nucleic Acids Res. 1982;10:7373-7385
The nucleotide sequence of the dnaA gene and the first part of the dnaN gene of Escherichia coli K-12.
Hansen, E. B., F. G. Hansen, and K. von Meyenburg.
Annu Rev Biochem. 1995;64:171-200.
DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
Kelman Z, O'Donnell M. ( 7574479 )
J Bacteriol. 1979;140(3):825-34.
Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication.
Kimura M, Miki T, Hiraga S, Nagata T, Yura T. ( 160413 )
Gene. 1984;28(2):159-70.
Structural analysis of the dnaA and dnaN genes of Escherichia coli.
Ohmori H, Kimura M, Nagata T, Sakakibara Y. ( 6234204 )
Plasmid. 1983;10(2):101-10.
Cloning and deletion mapping of the recF dnaN region of the Escherichia coli chromosome.
Ream LW, Clark AJ. ( 6356186 )
Mol Gen Genet. 1980;180(1):115-21.
Genetic and physical mapping of recF in Escherichia coli K-12.
Ream LW, Margossian L, Clark AJ, Hansen FG, von Meyenburg K. ( 6255290 )
Gene. 1981;13(1):47-55.
Organization and transcription of the dnaA and dnaN genes of Escherichia coli.
Sakakibara Y, Tsukano H, Sako T. ( 6453739 )
Mol Gen Genet. 1980;178(3):541-53.
A temperature-sensitive Escherichia coli mutant defective in DNA replication: dnaN, a new gene adjacent to the dnaA gene.
Sakakibara Y, Mizukami T. ( 6248733 )
Mol Gen Genet. 1980;179(3):521-6.
Coordinate expression of Escherichia coli dnaA and dnaN genes.
Sako T, Sakakibara Y. ( 6449652 )
Mol Gen Genet. 1980;180(2):267-73.
Identification of the dnaA and dnaN gene products of Escherichia coli.
Yuasa S, Sakakibara Y. ( 6258023 )
Proc Natl Acad Sci U S A. 1981;78:5391-5395
The dnaN gene codes for the subunit of DNA polymerase III holoenzyme of Escherichia coli.
Burgers, P. M. J., A. Kornberg, and Y. Sakakibara.

Sequences
Amino acid
FASTA format
0001 MKFTVEREHL LKPLQQVSGP LGGRPTLPIL GNLLLQVADG TLSLTGTDLE MEMVARVALV QPHEPGATTV 
0071 PARKFFDICR GLPEGAEIAV QLEGERMLVR SGRSRFSLST LPAADFPNLD DWQSEVEFTL PQATMKRLIE 
0141 ATQFSMAHQD VRYYLNGMLF ETEGEELRTV ATDGHRLAVC SMPIGQSLPS HSVIVPRKGV IELMRMLDGG 
0211 DNPLRVQIGS NNIRAHVGDF IFTSKLVDGR FPDYRRVLPK NPDKHLEAGC DLLKQAFARA AILSNEKFRG 
0281 VRLYVSENQL KITANNPEQE EAEEILDVTY SGAEMEIGFN VSYVLDVLNA LKCENVRMML TDSVSSVQIE 
0351 DAASQSAAYV VMPMRL

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gtgcttcatg cctgccgtaa gatcgagcag 
-070 ttgcgtgaag agagccacga tatcaaagaa gatttttcaa atttaatcag aacattgtca tcgtaaacct 
0001 atgaaattta ccgtagaacg tgagcattta ttaaaaccgc tacaacaggt gagcggtccg ttaggtggtc 
0071 gtcctacgct accgattctc ggtaatctgc tgttacaggt tgctgacggt acgttgtcgc tgaccggtac 
0141 tgatctcgag atggaaatgg tggcacgtgt tgcgctggtt cagccacacg agccaggagc gacgaccgtt 
0211 ccggcgcgca aattctttga tatctgccgt ggtctgcctg aaggcgcgga aattgccgtg cagctggaag 
0281 gtgaacggat gctggtacgc tccgggcgta gccgtttttc gctgtctacc ctgccagcgg cggatttccc 
0351 gaacctcgat gactggcaga gtgaagtcga atttaccctg ccgcaggcaa cgatgaagcg tctgattgaa 
0421 gcgacccagt tttctatggc gcatcaggac gttcgctatt acttaaatgg tatgctgttt gaaaccgaag 
0491 gtgaagaact gcgcaccgtg gcaaccgacg gccaccgtct ggcggtctgt tcaatgccaa ttggtcaatc 
0561 tttgccaagc cattcggtga tcgtaccgcg taaaggcgtg attgaactga tgcgtatgct cgacggcggc 
0631 gacaatccgc tgcgcgtaca gattggcagc aacaacattc gcgcccacgt tggcgacttt atcttcacct 
0701 ccaaactggt ggatggtcgc ttcccggatt atcgccgcgt tctgccgaag aacccggaca aacatctgga 
0771 agctggctgc gatctgctca agcaggcgtt tgctcgcgcg gcgattctct ctaacgagaa attccgcggc 
0841 gtacgtcttt atgtcagcga aaaccagctg aaaatcaccg ccaacaaccc ggaacaggaa gaagcggaag 
0911 agatcctcga cgttacctat agcggtgcgg agatggaaat cggcttcaac gtcagttatg tgctggatgt 
0981 tctgaacgcg ctgaaatgcg aaaacgtccg catgatgctg accgattcgg tttccagcgt gcagattgaa 
1051 gatgcggcca gccagagcgc ggcttatgtt gtcatgccaa tgagactgta atgtccctca cccgcttgtt 
1121 gatccgcgat ttccgcaaca ttgaaaccgc ggatctcgcc ttatctcccg gctttaactt tctggtaggt 
1191 gccaacggca g