MG1655
W3110
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Gene Report : recF
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL04 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  recF
Related strains
     Strains Search Search strains by gene name:  recF   Search strains by all related name:  recF b3700 ECK3692 f357 JW3677 uvrF

General information  (Go to Linear View:)
 Gene Name recF  
 Alternative name b3700,ECK3692,f357,JW3677,uvrF  
 Location, Length 3,878,171 - 3,879,244 (  -  ) ;   83.59 min ; 1074 (bp) ,   357 (aa) Go to Linear View
 Product gap repair protein  
 Operon Name dnaAN-recF  
 Note ssDNA and dsDNA binding, ATP binding; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006261 - DNA-dependent DNA replication; GO_process: GO:0006281 - DNA repair; GO_process: GO:0006310 - DNA recombination  
 Function regulator; DNA - replication, repair,restriction/modification  
 Gene Ontology GO:0000166; nucleotide binding ( recF )
GO:0003677; DNA binding ( recF )
GO:0003697; single-stranded DNA binding ( recF )
GO:0005515; protein binding ( recF )
GO:0005524; ATP binding ( recF )
GO:0005694; chromosome ( recF )
GO:0005737; cytoplasm ( recF )
GO:0006259; DNA metabolic process ( recF )
GO:0006260; DNA replication ( recF )
GO:0006281; DNA repair ( recF )
GO:0006974; response to DNA damage stimulus ( recF )
GO:0009432; SOS response ( recF )
 PID 1790135  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene recF ( EG10828 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) RECF_ECOLI ( P03016 )
    -  Protein name DNA replication and repair protein recF.  
    -  Synonyms  
    -  Gene name OrderedLocusNames=b3700, c4622, z5191, ECs4635;  

 Linear View (Whole Mode)
View Location
   3865.0  –  3890.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP recF (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain X, Structural Biochemistry Of Atp-Driven Dimerization And Dna Stimulated Activation Of Smc Atpases.  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST DNA replication and repair protein recF  
         PSI-BLAST DNA replication and repair protein recF
    nr     (database updated : 2007.02.20 )
         BLAST recombination protein F [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    AMIDATION    ATP_GTP_A    RECF_1    RECF_2     

Other Cross-Reference
    COG COG1195L 
    EcoCyc recF 

MMBR References
Nucleic Acids Res. 1987;15:771-784
DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy.
Adachi, T., M. Mizuuchi, A. Robinson, E. Appella, M. H. O'Dea, M. Gellert, and K. Mizuuchi.
Nucleic Acids Res. 1984;12:6389-6395
DNA sequence and transcription of the region upstream of the E. coli gyrB gene.
Adachi, T., R. Mizuuchi, R. Menzel, and M. Gellert.
Gene. 1986;43(3):183-96.
Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences.
Armengod ME, Lambies E. ( 3527871 )
J Bacteriol. 1990;172(11):6291-9.
Purification and preliminary characterization of the Escherichia coli K-12 recF protein.
Griffin TJ 4th, Kolodner RD. ( 2228960 )
J Bacteriol. 1994;176:7113-7114
The RecF pathway of homologous recombination can mediate the initiation of DNA damage-inducible replication of the Escherichia coli chromosome.
Asai, T., and T. Kogoma.
Proc Natl Acad Sci U S A. 1988;85:6592-6596
Properties of a mutant recA-encoded protein reveal a possible role for Escherichia coli recF-encoded protein in genetic recombination.
Madiraju, M. V., A. Templin, and A. J. Clark.
Plasmid. 1983;10(2):101-10.
Cloning and deletion mapping of the recF dnaN region of the Escherichia coli chromosome.
Ream LW, Clark AJ. ( 6356186 )
Mol Gen Genet. 1980;180(1):115-21.
Genetic and physical mapping of recF in Escherichia coli K-12.
Ream LW, Margossian L, Clark AJ, Hansen FG, von Meyenburg K. ( 6255290 )
Proc Natl Acad Sci U S A. 1984;81:4622-4626
Molecular analysis of the recF gene of Escherichia coli.
Blanar, M. A., S. J. Sandler, M.-E. Armengod, L. W. Ream, and A. J. Clark.
Mol Microbiol. 1996;19(4):871-80.
Overlapping functions for recF and priA in cell viability and UV-inducible SOS expression are distinguished by dnaC809 in Escherichia coli K-12.
Sandler SJ. ( 8820655 )
J Bacteriol. 1994;176:4011-4016
Mutational analysis of sequences in the recF gene of Escherichia coli K-12 that affect expression.
Sandler, S. J., and A. J. Clark.
Nucleic Acids Res. 1992;20:839-845
Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop.
Sandler, S. J., B. Chackerian, J. T. Li, and A. J. Clark.
ICN-UCLA Symp. Mol. Cell. Biol. 1980;19:137-159.
The origin of replication, oriC, of the Escherichia coli chromosome: genes near oriC and construction of oriC deletion mutations.
von Meyenburg, K., F. G. Hansen, E. Riise, H. E. N. Bergmans, M. Meijer, and W. Messer.
Mutat Res. 1997;381(1):111-5.
Involvement of recA and recF in the induced precise excision of Tn10 in Escherichia coli.
Chan A, Nagel R. ( 9403037 )
Biochimie. 1991;73(4):523-32.
rec genes and homologous recombination proteins in Escherichia coli.
Clark AJ. ( 1911953 )

Sequences
Amino acid
FASTA format
0001 MSLTRLLIRD FRNIETADLA LSPGFNFLVG ANGSGKTSVL EAIYTLGHGR AFRSLQIGRV IRHEQEAFVL 
0071 HGRLQGEERE TAIGLTKDKQ GDSKVRIDGT DGHKVAELAH LMPMQLITPE GFTLLNGGPK YRRAFLDWGC 
0141 FHNEPGFFTA WSNLKRLLKQ RNAALRQVTR YEQLRPWDKE LIPLAEQIST WRAEYSAGIA ADMADTCKQF 
0211 LPEFSLTFSF QRGWEKETEY AEVLERNFER DRQLTYTAHG PHKADLRIRA DGAPVEDTLS RGQLKLLMCA 
0281 LRLAQGEFLT RESGRRCLYL IDDFASELDD ERRGLLASRL KATQSQVFVS AISAEHVIDM SDENSKMFTV 
0351 EKGKITD

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             aaaacgtccg catgatgctg accgattcgg 
-070 tttccagcgt gcagattgaa gatgcggcca gccagagcgc ggcttatgtt gtcatgccaa tgagactgta 
0001 atgtccctca cccgcttgtt gatccgcgat ttccgcaaca ttgaaaccgc ggatctcgcc ttatctcccg 
0071 gctttaactt tctggtaggt gccaacggca gtggcaaaac cagcgtgctg gaagccatct atacgctcgg 
0141 ccatggtcgg gcgtttcgca gtttgcagat tggtcgcgtc attcgccatg agcaggaggc gtttgttctc 
0211 cacgggcgat tacagggcga agagcgcgag acagcgattg gcttaaccaa agacaaacag ggcgacagca 
0281 aagtccgcat cgacggtaca gacgggcata aggtcgcgga actggcgcac ctgatgccaa tgcagttgat 
0351 aacgccagaa gggtttactt tactcaacgg cggccccaaa tacagaagag cattcctcga ctggggatgc 
0421 tttcacaacg aacccggatt tttcaccgcc tggagcaatc tcaagcgatt gctcaagcag cgcaatgcgg 
0491 cgctgcgcca ggtgacacgt tacgaacagc tacgcccgtg ggataaagag ctgatcccgc tggcggagca 
0561 aatcagcacc tggcgcgcgg agtatagcgc cggtatcgcg gccgatatgg ctgatacctg taagcaattt 
0631 ctccctgagt tttctctgac tttctctttc cagcgcggct gggagaaaga gacagaatat gctgaggtgc 
0701 tggaacgtaa ttttgaacgc gatcgccagc taacctacac cgcgcacggc ccgcacaaag cggacttacg 
0771 cattcgcgcc gacggtgcgc cggtggaaga taccttatcg cgtgggcagc ttaagctgtt gatgtgcgcc 
0841 ttacgtctgg cgcaaggaga gttcctcacc cgtgaaagcg ggcggcggtg tctctacctg atagatgatt 
0911 ttgcctctga gcttgatgat gagcgtcgcg ggctgcttgc cagccgctta aaagcgacgc aatcacaggt 
0981 ctttgtcagc gcgatcagtg ctgaacacgt tatagacatg tcggacgaaa attcgaagat gtttaccgtg 
1051 gaaaagggta aaataacgga ttaacccaag tataaatgag cgagaaacgt tgatgtcgaa ttcttatgac 
1121 tcctccagta tcaaagtcct gaaagggctg gatgcggtgc gtaagcgccc gggt