MG1655
W3110
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Gene Report : trmH
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL07 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  trmH
Related strains
     Strains Search Search strains by gene name:  trmH   Search strains by all related name:  trmH b3651 ECK3641 JW3626 o229 spoU

General information  (Go to Linear View:)
 Gene Name trmH  
 Alternative name b3651,ECK3641,JW3626,o229,spoU  
 Location, Length 3,822,538 - 3,823,227 (  +  ) ;   82.39 min ; 690 (bp) ,   229 (aa) Go to Linear View
 Product tRNA mG18-2'-O-methyltransferase, SAM-dependent  
 Operon Name rpoZ-spoT-trmH-recG  
 Note GO_process: GO:0009451 - RNA modification  
 Function enzyme; tRNA modification  
 Gene Ontology GO:0003723; RNA binding ( trmH )
GO:0005737; cytoplasm ( trmH )
GO:0006396; RNA processing ( trmH )
GO:0008033; tRNA processing ( trmH )
GO:0008168; methyltransferase activity ( trmH )
GO:0008173; RNA methyltransferase activity ( trmH )
GO:0009020; tRNA (guanosine-2'-O-)-methyltransferase activity ( trmH )
GO:0016740; transferase activity ( trmH )
 PID 1790083  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) TRMH_ECOLI ( P19396 )
    -  Protein name tRNA (Guanosine-2'-O-)-methyltransferase  
    -  Synonyms EC 2.1.1.34, tRNA [GM18] methyltransferase  
    -  Gene name OrderedLocusNames=b3651, z5077, ECs4526, SF3691, S4078;  

 Linear View (Whole Mode)
View Location
   3810.0  –  3835.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP trmH (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of A. Aeolicus TrmhSPOU TRNA MODIFYING Enzyme  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST tRNA guanosine-2'-O-methyltransferase (tRNA [Gm18] methyltransferase)  
         PSI-BLAST tRNA guanosine-2'-O-methyltransferase (tRNA [Gm18] methyltransferase)
    nr     (database updated : 2007.02.20 )
         BLAST putative RNA methylase [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    COG COG0566J 
    EcoCyc trmH 

MMBR References
Nucleic Acids Res. 1993;21(23):5519
SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases.
Koonin EV, Rudd KE. ( 8265370 )
Nucleic Acids Res. 1997;25:4093-4097
The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity.
Persson, B. C., G. Jager, and C. Gustafsson.

Sequences
Amino acid
FASTA format
0001 MNPTRYARIC EMLARRQPDL TVCMEQVHKP HNVSAIIRTA DAVGVHEVHA VWPGSRMRTM ASAAAGSNSW 
0071 VQVKTHRTIG DAVAHLKGQG MQILATHLSD NAVDFREIDY TRPTCILMGQ EKTGITQEAL ALADQDIIIP 
0141 MIGMVQSLNV SVASALILYE AQRQRQNAGM YLRENSMLPE AEQQRLLFEG GYPVLAKVAK RKGLPYPHVN 
0211 QQGEIEADAD WWATMQAAG

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             TCTGACCGCT CGTGACCGTG TGCATCTGGC 
-070 GAATATCATG CGCAAAATCC GCGTGATGCC AGACGTGATT AAAGTCACCC GAAACCGAAA TTAATGTTTT 
0001 atgaacccaa cacgttatgc acgcatctgc gaaatgctcg ccaggcggca gcctgatctg accgtctgca 
0071 tggagcaggt ccacaaacct cataacgttt ctgcgattat tcgtaccgca gatgccgttg gcgtacatga 
0141 agttcacgcc gtctggcctg gtagccgcat gcgcaccatg gcttcggcag cggcgggtag taacagctgg 
0211 gtacaggtga aaacacaccg caccattggc gatgccgtcg ctcatctcaa aggccagggc atgcagattc 
0281 tggcaaccca tctttctgat aacgctgtcg atttccgcga aattgattac actcgcccga cctgcatttt 
0351 gatgggacag gagaaaacgg gcatcacgca ggaagcattg gccctggcgg atcaggacat catcattccg 
0421 atgatcggca tggtgcagtc gctgaatgtt tccgttgcct cagccctcat tctttacgaa gcccagcgtc 
0491 agcggcaaaa tgcaggcatg tacctgcgtg aaaacagcat gttgccggaa gcagagcaac aacgcctgtt 
0561 gtttgaaggc ggctatccgg tgctggcgaa agtcgcaaaa cgcaaaggcc tgccttatcc ccacgtcaat 
0631 cagcaaggcg agatcgaagc tgatgccgac tggtgggcta ctatgcaggc tgcagggtaa GTGCCATGAA 
0701 AGGTCGCCTG TTAGATGCTG TCCCACTCAG TTCCCTAACG GGCGTTGGCG CAGCACTTAG TAACAAACTG 
0771 GCGAAAATCA ACCTGCATAC