|
PEC Original Annotations
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Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
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Deletion
|
OCL07
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
rph
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
rph
Search strains by all related name:
rph b3643 ECK3633 f228 JW3618
|
General information (Go to Linear View: )
|
|
Gene Name
|
rph
|
|
Alternative name
|
b3643,ECK3633,f228,JW3618
|
|
Location, Length
|
3,813,886 - 3,814,572
(
-
)
;
82.20
min
;
687
(bp) ,
228
(aa)
|
|
Product
|
defective ribonuclease PH
|
|
Operon Name
|
rph-pyrE
|
|
Note
|
defective ribonuclease PH; enzyme; degradation of RNA; RNase PH; MG1655 has the rph-1 C-terminal frameshift mutation caused by a one base pair deletion of a G of the first base of codon 223 that severely reduces Rph activity; rph-1 mutation is polar on the downstream pyrE gene, reducing PyrE activity and causing partial pyrimidine starvation when grown in minimal medium; this mutant ribonuclease PH is shorter (228 aa) and has a C-terminal sequence of ESNPL replacing GIESIVATQKAALAN; GO_process: GO:0006401 - RNA catabolic process
|
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Function
|
|
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Gene Ontology
|
GO:0000049
;
tRNA binding ( rph )
GO:0000175
;
3'-5'-exoribonuclease activity ( rph )
GO:0003723
;
RNA binding ( rph )
GO:0004549
;
tRNA-specific ribonuclease activity ( rph )
GO:0006396
;
RNA processing ( rph )
GO:0008033
;
tRNA processing ( rph )
GO:0009022
;
tRNA nucleotidyltransferase activity ( rph )
GO:0016740
;
transferase activity ( rph )
GO:0016779
;
nucleotidyltransferase activity ( rph )
|
|
PID
|
157672248
|
EC number
(KEGG Pathway)
|
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
rph
(
EG10863
)
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Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
RNPH_ECOLI
(
P03842
)
|
|
- Protein name
|
Ribonuclease PH
|
|
- Synonyms
|
EC 2.7.7.56, RNase PH, tRNA nucleotidyltransferase
|
|
- Gene name
|
Name=rph; OrderedLocusNames=b3643;
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Homology Analysis
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BLAST
|
|
Bacteria
|
|
GTOP
|
rph
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of The Trna Processing Enzyme Rnase Ph From Aquifex Aeolicus
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Ribonuclease PH
|
|
PSI-BLAST
|
Ribonuclease PH
|
|
nr
(database updated :
2007.02.20
)
|
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BLAST
|
ribonuclease PH [Escherichia coli K12]
|
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
CK2_PHOSPHO_SITE
MYRISTYL
RIBONUCLEASE_PH
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MMBR References
|
J Bacteriol. 1993;175:3401-3407
The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels.
Jensen, K. F.
|
J Biol Chem. 1992;267:17147-17152
Overexpression and rapid purification of the orfE/rph gene product, RNase PH of Escherichia coli.
Jensen, K. F., J. T. Andersen, and P. Poulsen.
|
J Biol Chem. 1992;267:16015-16018
RNase PH is essential for tRNA processing and viability in RNase-deficient Escherichia coli cells.
Kelly, K. O., N. B. Reuven, Z.-W. Li, and M. P. Deutscher.
|
J Bacteriol. 1991;173(17):5589-91.
Escherichia coli orfE (upstream of pyrE) encodes RNase PH.
Ost KA, Deutscher MP.
(
1885537
)
|
Eur J Biochem. 1992;206:381-390
Attenuation in the rph-pyrE operon of Escherichia coli and processing of the dicistronic mRNA.
Andersen, J. T., P. Poulsen, and K. F. Jensen.
|
| Sequences |
Amino acid
FASTA format
|
0001 MRPAGRSNNQ VRPVTLTRNY TKHAEGSVLV EFGDTKVLCT ASIEEGVPRF LKGQGQGWIT AEYGMLPRST
0071 HTRNAREAAK GKQGGRTMEI QRLIARALRA AVDLKALGEF TITLDCDVLQ ADGGTRTASI TGACVALVDA
0141 LQKLVENGKL KTNPMKGMVA AVSVGIVNGE AVCDLEYVED SAAETDMNVV MTEDGRIIEV QGTAEGEPFT
0211 HEELLILLAL ARGESNPL
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gtggaaggat tatagccatc gatgccttgt
-070 caggatagga ataaccgccg gaagtccgta taatgcgcag ccacatttgt ttcaagccgg agatttcaat
0001 atgcgtccag caggccgtag caataatcag gtgcgtcccg ttaccctgac tcgtaactat acaaaacatg
0071 cagaaggctc ggtgctggtc gaatttggcg ataccaaagt gttgtgtacc gcctctattg aagaaggcgt
0141 gccgcgcttc ctgaaaggtc agggccaggg ctggatcacc gcagagtacg gcatgctgcc acgttctacc
0211 cacacccgta acgctcgtga agcggcgaaa ggtaagcagg gtggacgcac aatggaaatc cagcgtctga
0281 tcgcccgtgc tcttcgcgcg gcagtagatt tgaaagcgct gggtgagttc accattacgc tggactgcga
0351 cgtgcttcag gctgatggtg gcacgcgtac cgcgtcgatt acgggtgcct gcgtggcgct ggtagatgcg
0421 ctacagaagc tggtggaaaa cggcaagctg aaaaccaatc cgatgaaagg gatggtagcc gcagtttctg
0491 tcggaattgt gaacggcgaa gcggtttgcg atctggaata cgttgaagac tctgccgcag agaccgacat
0561 gaacgtagtg atgaccgaag acgggcgcat cattgaagtg caggggacgg cagaaggcga gccgttcacc
0631 catgaagagc tactcatctt gttggctctg gcccgagggg aatcgaatcc attgtagcga cgcagaaggc
0701 ggcgctggca aactgatttt taaggcgact gatgagtcgc cttttttttg tctgtagaaa agtaagatga
0771 ggagcgaagg catgaaa
|
