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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCL07
(D)
|
|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
pyrE
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Related strains
|
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Strains Search
|
Search strains by gene name:
pyrE
Search strains by all related name:
pyrE b3642 ECK3632 f213 JW3617
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General information (Go to Linear View: )
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Gene Name
|
pyrE
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Alternative name
|
b3642,ECK3632,f213,JW3617
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Location, Length
|
3,813,150 - 3,813,791
(
-
)
;
82.19
min
;
642
(bp) ,
213
(aa)
|
|
Product
|
orotate phosphoribosyltransferase
|
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Operon Name
|
rph-pyrE
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006221 - pyrimidine nucleotide biosynthetic process
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Function
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enzyme; Pyrimidine ribonucleotide biosynthesis
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Gene Ontology
|
GO:0000287
;
magnesium ion binding ( pyrE )
GO:0004588
;
orotate phosphoribosyltransferase activity ( pyrE )
GO:0005515
;
protein binding ( pyrE )
GO:0006221
;
pyrimidine nucleotide biosynthetic process ( pyrE )
GO:0009116
;
nucleoside metabolic process ( pyrE )
GO:0016740
;
transferase activity ( pyrE )
GO:0016757
;
transferase activity, transferring glycosyl groups ( pyrE )
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|
PID
|
1790073
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EC number
(KEGG Pathway)
|
2.4.2.10
|
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
pyrE
(
EG10808
)
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Number of removed
|
1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
|
PYRE_ECOLI
(
P00495
)
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- Protein name
|
Orotate phosphoribosyltransferase
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- Synonyms
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EC 2.4.2.10, OPRT, OPRTase
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- Gene name
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Name=pyrE; OrderedLocusNames=b3642, c4466;
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MMBR References
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J Bacteriol. 1993;175:3401-3407
The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels.
Jensen, K. F.
|
EMBO J. 1984;3:1783-1790
Structure of the Escherichia coli pyrE operon and control of pyrE expression by a UTP modulated intercistronic attenuation.
Poulsen, P., F. Bonekamp, and K. F. Jensen.
|
Mol Microbiol. 1989;3(3):393-404.
Molecular and mutational analysis of three genes preceding pyrE on the Escherichia coli chromosome.
Poulsen P, Andersen JT, Jensen KF.
(
2664418
)
|
Res Microbiol. 1991;142(2-3):283-8.
Three genes preceding pyrE on the Escherichia coli chromosome are essential for survival and normal cell morphology in stationary culture and at high temperature.
Poulsen P, Jensen KF.
(
1925027
)
|
Eur J Biochem. 1983;135:223-229
Nucleotide sequence of the Escherichia coli pyrE gene and of the DNA in front of the protein-coding region.
Poulsen, P., K. F. Jensen, P. Valentin-Hansen, P. Carlsson, and L. G. Lundberg.
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Gene. 1980;9(3-4):321-36.
Cloning of the dut (deoxyuridine triphosphatase) gene of Escherichia coli.
Taylor AF, Siliciano PG, Weiss B.
(
6104620
)
|
Eur J Biochem. 1992;206:381-390
Attenuation in the rph-pyrE operon of Escherichia coli and processing of the dicistronic mRNA.
Andersen, J. T., P. Poulsen, and K. F. Jensen.
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| Sequences |
Amino acid
FASTA format
|
0001 MKPYQRQFIE FALSKQVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLALLG RFYAEALVDS GIEFDLLFGP
0071 AYKGIPIATT TAVALAEHHD LDLPYCFNRK EAKDHGEGGN LVGSALQGRV MLVDDVITAG TAIRESMEII
0141 QANGATLAGV LISLDRQERG RGEISAIQEV ERDYNCKVIS IITLKDLIAY LEEKPEMAEH LAAVKAYREE
0211 FGV
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ttgtagcgac gcagaaggcg gcgctggcaa
-070 actgattttt aaggcgactg atgagtcgcc ttttttttgt ctgtagaaaa gtaagatgag gagcgaaggc
0001 atgaaaccat atcagcgcca gtttattgaa tttgcgctta gcaagcaggt gttaaagttt ggcgagttta
0071 cgctgaaatc cgggcgcaaa agcccctatt tcttcaacgc cgggctgttt aataccgggc gcgatctggc
0141 actgttaggc cgtttttacg ctgaagcgtt ggtggattcc ggcattgagt tcgatctgct gtttggccct
0211 gcttacaaag ggatcccgat tgccaccaca accgctgtgg cactggcgga gcatcacgac ctggacctgc
0281 cgtactgctt taaccgcaaa gaagcaaaag accacggtga aggcggcaat ctggttggta gcgcgttaca
0351 aggacgcgta atgctggtag atgatgtgat caccgccgga acggcgattc gcgagtcgat ggagattatt
0421 caggccaatg gcgcgacgct tgctggcgtg ttgatttcgc tcgatcgtca ggaacgcggg cgcggcgaga
0491 tttcggcgat tcaggaagtt gagcgtgatt acaactgcaa agtgatctct atcatcaccc tgaaagacct
0561 gattgcttac ctggaagaga agccggaaat ggcggaacat ctggcggcgg ttaaggccta tcgcgaagag
0631 tttggcgttt aaagaaactc gccggatgaa aagtcatccg gcgtcatatt actgcaactg tgccgcaatt
0701 agcggccagc gggcgtcaaa atcatccgtc gggcggtatt ta
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