MG1655
W3110
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Gene Report : malS
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion 2-2 (LD)  ,  OCL09,11-1 (LD 2-2'_CRS) (D)  ,  OCL09,11-2 (LD 2-2'_P/O) (D)  ,  OCL10 (D)  ,  OCL11 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  malS
Related strains
     Strains Search Search strains by gene name:  malS   Search strains by all related name:  malS b3571 ECK3560 JW3543

General information  (Go to Linear View:)
 Gene Name malS  
 Alternative name b3571,ECK3560,JW3543  
 Location, Length 3,735,520 - 3,737,550 (  +  ) ;   80.51 min ; 2031 (bp) ,   676 (aa) Go to Linear View
 Product alpha-amylase  
 Operon Name malS  
 Note GO_component: GO:0042597 - periplasmic space; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0000272 - polysaccharide catabolic process; GO_process: GO:0006508 - proteolysis  
 Function enzyme; Degradation of polysaccharides  
 Gene Ontology GO:0003824 ; catalytic activity ( malS )
GO:0004556 ; alpha-amylase activity ( malS )
GO:0005509 ; calcium ion binding ( malS )
GO:0005975 ; carbohydrate metabolic process ( malS )
GO:0008152 ; metabolic process ( malS )
GO:0016787 ; hydrolase activity ( malS )
GO:0016798 ; hydrolase activity, acting on glycosyl bonds ( malS )
GO:0042597 ; periplasmic space ( malS )
GO:0043169 ; cation binding ( malS )
GO:0046872 ; metal ion binding ( malS )
 PID 1789995  
 EC number
  (KEGG Pathway)
 
3.2.1.1  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene malS ( EG11316 )  
    Number of removed 17 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) AMY1_ECOLI ( P25718 )
    -  Protein name Alpha-amylase precursor  
    -  Synonyms EC 3.2.1.1, 1,4-alpha-D-glucan glucanohydrolase  
    -  Gene name Name=malS; OrderedLocusNames=b3571;  

 Linear View (Whole Mode)
View Location
   3725.0  –  3750.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP malS (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain , Cyclomaltodextrin Glucanotransferase  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Alpha-amylase precursor  
         PSI-BLAST Alpha-amylase precursor
    nr     (database updated : 2007.02.20 )
         BLAST periplasmic alpha-amylase precursor [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL     

Other Cross-Reference
    EcoCyc malS 

MMBR References
J Biol Chem. 1986;261:2946-2953
Alpha-amylase of Escherichia coli, mapping and cloning of the structural gene, malS, and identification of its product as a periplasmic protein.
Freundlieb, S., and W. Boos.
J Biol Chem. 1992;267:5148-5154
Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by MalS.
Schneider, E., S. Freundlieb, S. Tapio, and W. Boos.

Sequences
Amino acid
FASTA format
0001 MKLAACFLTL LPGFAVAASW TSPGFPAFSE QGTGTFVSHA QLPKGTRPLT LNFDQQCWQP ADAIKLNQML 
0071 SLQPCSNTPP QWRLFRDGEY TLQIDTRSGT PTLMISIQNA AEPVASLVRE CPKWDGLPLT VDVSATFPEG 
0141 AAVRDYYSQQ IAIVKNGQIM LQPAATSNGL LLLERAETDT SAPFDWHNAT VYFVLTDRFE NGDPSNDQSY 
0211 GRHKDGMAEI GTFHGGDLRG LTNKLDYLQQ LGVNALWISA PFEQIHGWVG GGTKGDFPHY AYHGYYTQDW 
0281 TNLDANMGNE ADLRTLVDSA HQRGIRILFD VVMNHTGYAT LADMQEYQFG ALYLSGDEVK KSLGERWSDW 
0351 KPAAGQTWHS FNDYINFSDK TGWDKWWGKN WIRTDIGDYD NPGFDDLTMS LAFLPDIKTE STTASGLPVF 
0421 YKNKMDTHAK AIDGYTPRDY LTHWLSQWVR DYGIDGFRVD TAKHVELPAW QQLKTEASAA LREWKKANPD 
0491 KALDDKPFWM TGEAWGHGVM QSDYYRHGFD AMINFDYQEQ AAKAVDCLAQ MDTTWQQMAE KLQGFNVLSY 
0561 LSSHDTRLFR EGGDKAAELL LLAPGAVQIF YGDESSRPFG PTGSDPLQGT RSDMNWQDVS GKSAASVAHW 
0631 QKISQFRARH PAIGAGKQTT LLLKQGYGFV REHGDDKVLV VWAGQQ

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CCTTGCTTCT CATCCTGTGG ACTTACCGCT 
-070 CAGGGATGAG TTTTGTTTGG CTTATCGCTG GCAAACTGTC TGAAATCGCA GCAATAAGGA CTCATCCGCC 
0001 atgaaactcg ccgcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg 
0071 ggtttcccgc ctttagcgaa caggggacag gaacatttgt cagccacgcg cagttgccca aaggtacgcg 
0141 tccactaacg ctaaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa tcagatgctt 
0211 tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga cggcgaatat acgctgcaaa 
0281 tagacacccg ctccggtacg ccaacattga tgatttccat ccagaacgcc gccgaaccgg tagcaagcct 
0351 ggtccgtgaa tgcccgaaat gggatggatt accgctcaca gtggatgtca gcgccacttt cccggaagga 
0421 gccgccgtac gggattatta cagccagcaa attgcgatag tgaagaacgg tcaaataatg ttacaacccg 
0491 ctgccaccag caacggttta ctcctgctgg aacgggcaga aactgacaca tccgcccctt tcgactggca 
0561 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga ccagagttac 
0631 ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga tttacgcggc ctgaccaaca 
0701 aactggatta cctccagcag ttgggcgtta atgctttatg gataagcgcc ccatttgagc aaattcacgg 
0771 ctgggtcggc ggcggtacaa aaggcgattt cccgcattat gcctaccacg gttattacac acaggactgg 
0841 acgaatcttg atgccaatat gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg 
0911 gtattcgtat tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta 
0981 tcagtttggc gcgttatatc tttctggtga cgaagtgaaa aaatcgctgg gtgaacgctg gagcgactgg 
1051 aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt cagcgacaaa acaggctggg 
1121 ataaatggtg gggaaaaaac tggatcagaa cggatatcgg cgattacgac aatcctggat tcgacgatct 
1191 cactatgtcg ctagcctttt tgccggatat caaaaccgaa tcaactaccg cttctggtct gccggtgttc 
1261 tataaaaaca aaatggatac ccacgccaaa gccattgacg gctatacgcc gcgcgattac ttaacccact 
1331 ggttaagtca gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt 
1401 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc taaccccgac 
1471 aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca cggcgtgatg caaagtgact 
1541 actatcgcca cggcttcgat gcgatgatca atttcgatta tcaggagcag gcggcgaaag cagtcgactg 
1611 tctggcgcag atggatacga cctggcagca aatggcggag aaattgcagg gtttcaacgt gttgagctac 
1681 ctctcgtcgc atgatacccg cctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc 
1751 caggcgcggt acaaatcttt tatggtgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct 
1821 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccagcgt cgcgcactgg 
1891 cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa acaaacgaca cttttgctga 
1961 agcagggcta cggctttgtt cgtgagcatg gcgacgataa agtgctggtc gtctgggcag ggcaacagta 
2031 aCTTTTCCGG CTTCCCGTTC GTCAGTACCT CGGGAAGCCG CCAACCAGGA TAAAATGTCA GCCCTAATCA 
2101 GCGTTGCAGG ATAAAGCACC GCTCACTCTT C