|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
9-1
(LD)
,
OCR36,37-6 (LD 9C::Ap)
(D)
,
OCR37
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
mhpD
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
mhpD
Search strains by all related name:
mhpD b0350 ECK0347 JW0341 mhpS o271
|
General information (Go to Linear View: )
|
|
Gene Name
|
mhpD
|
|
Alternative name
|
b0350,ECK0347,JW0341,mhpS,o271
|
|
Location, Length
|
371,339 - 372,148
(
+
)
;
8.00
min
;
810
(bp) ,
269
(aa)
|
|
Product
|
2-keto-4-pentenoate hydratase
|
|
Operon Name
|
mhpABCDFE
|
|
Note
|
GO_process: GO:0019395 - fatty acid oxidation
|
|
Function
|
enzyme; Degradation of small molecules: Carbon compounds
|
|
Gene Ontology
|
GO:0003824
;
catalytic activity ( mhpD )
GO:0006725
;
aromatic compound metabolic process ( mhpD )
GO:0008152
;
metabolic process ( mhpD )
GO:0016829
;
lyase activity ( mhpD )
GO:0019439
;
aromatic compound catabolic process ( mhpD )
|
|
PID
|
87081722
|
EC number
(KEGG Pathway)
|
4.2.1.80
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
mhpD
(
EG14274
)
|
|
Number of removed
|
1 aa cleaved
|
|
SWISS-PROT
(
Show details
[
1
more]
)
|
Entry name(Acc.no)
|
MHPD_ECOLI
(
P77608
;
P71205; P77045
)
|
|
- Protein name
|
2-keto-4-pentenoate hydratase
|
|
- Synonyms
|
EC 4.2.1.-, 2-hydroxypentadienoic acid hydratase
|
|
- Gene name
|
Name=mhpD; OrderedLocusNames=b0350;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
mhpD
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of 2-Hydroxypentadienoic Acid Hydratase From Escherichia Coli
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
2-keto-4-pentenoate hydratase
|
|
PSI-BLAST
|
2-keto-4-pentenoate hydratase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
probable hydratase (EC 4.2.1.-) mhpD - Escherichia coli (strain K-12)
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
AMIDATION
|
|
MMBR References
|
J Bacteriol. 1997;179:2573-2581
Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12.
Ferrandez, A., J. L. Garcia, and E. Diaz.
|
Ph.D. thesis. University of Minnesota, Minneapolis-St. Paul. 1983
The degradation of 3-phenylpropionic acid by Escherichia coli.
Burlingame, R. P.
|
J Bacteriol. 1986;168(1):55-64.
Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation.
Burlingame RP, Wyman L, Chapman PJ.
(
3531186
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MTKHTLEQLA ADLRRAAEQG EAIAPLRDLI GIDNAEAAYA IQHINVQHDV AQGRRVVGRK VGLTHPKVQQ
0071 QLGVDQPDFG TLFADMCYGD NEIIPFSRVL QPRIEAEIAL VLNRDLPATD ITFDELYNAI EWVLPALEVV
0141 GSRIRDWSIQ FVDTVADNAS CGVYVIGGPA QRPAGLDLKN CAMKMTRNNE EVSSGRGSEC LGHPLNAAVW
0211 LARKMASLGE PLRTGDIILT GALGPMVAVN AGDRFEAHIE GIGSVAATFS SAAPKGSLS
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCATATCTTC CGCGACTGTG GTCACTGGGC
-070 GCAGTGGGAA CATGCCGACG CTTTCAATCA ACTGGTGCTG AATTTCCTCG CACGCCCTTA AGGAATGGTC
0001 atgacgaagc atactcttga gcaactggcg gcggatttac gccgcgccgc agagcagggc gaagcgattg
0071 caccgctgcg cgatctgatt ggtatcgata acgctgaagc ggcttacgcc attcagcaca taaatgtgca
0141 acatgacgtt gcgcaggggc gtcgcgtggt agggcgtaaa gtgggcctga cacatccgaa agtgcaacaa
0211 caactgggcg ttgatcaacc ggattttggg acgttatttg ccgacatgtg ttatggcgat aacgaaatca
0281 ttcctttttc ccgtgttctg caaccccgca ttgaagcgga gatcgcactg gtgttgaacc gcgatttgcc
0351 cgcaaccgat atcaccttcg acgaattgta taacgccatt gaatgggtac ttccggcgct ggaagtggtg
0421 gggagccgca ttcgcgactg gtcgattcag tttgtcgata ccgtggcaga taacgcctcc tgtggggtgt
0491 atgtcatcgg cggtccggcg caacgtccgg cggggttaga cctgaaaaac tgcgccatga agatgacgcg
0561 taataacgaa gaggtttcta gcgggcgcgg cagcgaatgc ctgggacatc cgcttaatgc ggccgtctgg
0631 ctggcacgca aaatggccag tctgggtgaa ccgctgcgca ccggagatat cattcttacc ggggcattag
0701 gtccgatggt ggcggtgaat gcgggcgatc gttttgaagc ccatattgaa ggcataggtt cagttgctgc
0771 gacattttca agcgcagccc caaaaggaag tctgtcatga GTAAGCGTAA AGTCGCCATT ATCGGTTCTG
0841 GCAACATTGG TACCGATCTG ATGATTAAAA TTTTGCGTCA CGGTCAGCAT CTGGAGATGG CGGTGATGGT
|
