|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
|
|
|
Deletion
|
9-1
(LD)
,
OCR36,37-6 (LD 9C::Ap)
(D)
,
OCR37
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
mhpB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
mhpB
Search strains by all related name:
mhpB b0348 ECK0345 JW0339
|
General information (Go to Linear View: )
|
|
Gene Name
|
mhpB
|
|
Alternative name
|
b0348,ECK0345,JW0339
|
|
Location, Length
|
369,501 - 370,445
(
+
)
;
7.96
min
;
945
(bp) ,
314
(aa)
|
|
Product
|
2,3-dihydroxyphenylpropionate 1,2-dioxygenase
|
|
Operon Name
|
mhpABCDFE
|
|
Note
|
GO_process: GO:0019395 - fatty acid oxidation
|
|
Function
|
enzyme; Degradation of small molecules: Carbon compounds
|
|
Gene Ontology
|
GO:0005506
;
iron ion binding ( mhpB )
GO:0006118
;
electron transport ( mhpB )
GO:0006725
;
aromatic compound metabolic process ( mhpB )
GO:0008198
;
ferrous iron binding ( mhpB )
GO:0016491
;
oxidoreductase activity ( mhpB )
GO:0016702
;
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen ( mhpB )
GO:0019439
;
aromatic compound catabolic process ( mhpB )
|
|
PID
|
1786544
|
EC number
(KEGG Pathway)
|
1.13.11.-
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
mhpB
(
EG20274
)
|
|
Number of removed
|
0 aa cleaved
|
|
SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
MHPB_ECOLI
(
P54711
;
P77048; P77461
)
|
|
- Protein name
|
2,3-dihydroxyphenylpropionate 1,2-dioxygenase
|
|
- Synonyms
|
EC 1.13.11.-
|
|
- Gene name
|
Name=mhpB; OrderedLocusNames=b0348, z0446, ECs0403;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
mhpB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain B, Three-Dimensional Structure Of Ligab
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
2,3-dihydroxyphenylpropionate 1,2-dioxygenase
|
|
PSI-BLAST
|
2,3-dihydroxyphenylpropionate 1,2-dioxygenase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
2,3-dihydroxyphenylpropionate 1,2-dioxygenase [Escherichia coli O157:H7 EDL933]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
MYRISTYL
|
|
MMBR References
|
J Bacteriol. 1997;179:2573-2581
Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12.
Ferrandez, A., J. L. Garcia, and E. Diaz.
|
J Bacteriol. 1996;178:5249-5256
Catechol dioxygenases from Escherichia coli (MhpB) and Alcaligenes eutrophus (MpcI): sequence analysis and biochemical properties of a third family of extradiol dioxygenases.
Spence, E. L., M. Kawamukai, J. Sanvoisin, H. Braven, and T. D. Bugg.
|
Ph.D. thesis. University of Minnesota, Minneapolis-St. Paul. 1983
The degradation of 3-phenylpropionic acid by Escherichia coli.
Burlingame, R. P.
|
J Bacteriol. 1986;168(1):55-64.
Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation.
Burlingame RP, Wyman L, Chapman PJ.
(
3531186
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MHAYLHCLSH SPLVGYVDPA QEVLDEVNGV IASARERIAA FSPELVVLFA PDHYNGFFYD VMPPFCLGVG
0071 ATAIGDFGSA AGELPVPVEL AEACAHAVMK SGIDLAVSYC MQVDHGFAQP LEFLLGGLDK VPVLPVFING
0141 VATPLPGFQR TRMLGEAIGR FTSTLNKRVL FLGSGGLSHQ PPVPELAKAD AHMRDRLLGS GKDLPASERE
0211 LRQQRVISAA EKFVEDQRTL HPLNPIWDNQ FMTLLEQGRI QELDAVSNEE LSAIAGKSTH EIKTWVAAFA
0281 AISAFGNWRS EGRYYRPIPE WIAGFGSLSA RTEN
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCCATTCCGC AAACCCTGGG CAAGACCCTG
-070 AATAAACTGG CGTCGGTGAT GACGCTGACC CGCCCTGATG CCGACGTTTC TGTCGAAAAG GTAGCCTGAT
0001 atgcacgctt atcttcactg tctttcccac tcgccgctgg tggggtatgt cgacccggcg caagaggtgc
0071 tcgatgaggt caatggcgtg attgccagcg cccgcgagcg tattgcggca ttctcccctg aactggtggt
0141 gctgtttgcg ccagatcact acaacggctt tttctatgac gtgatgccac cgttctgttt aggcgttgga
0211 gcgacggcaa ttggtgattt cggcagtgcg gcaggagagc tgcccgtgcc tgtggagctg gcggaggcct
0281 gtgcgcatgc cgtcatgaag agcgggatcg atcttgccgt ttcttactgt atgcaggtgg accacgggtt
0351 cgcccagccg ctggagttcc tgctcggtgg gctggataag gtgccagttc tgcctgtgtt catcaacggt
0421 gtcgccacgc cgctgcccgg tttccagcgt acccgcatgt tgggtgaagc cattggacgt ttcaccagca
0491 ctctcaataa acgcgtgctg ttcctgggtt ccggtgggct ttcccatcag ccgccggtgc ccgaactggc
0561 gaaagccgat gcccatatgc gcgaccgtct gttggggagc gggaaagatt tacccgccag tgagcgcgaa
0631 ttgcgtcagc aacgggtgat tagcgccgct gagaagtttg ttgaggatca gagaacgctg catccgctca
0701 acccgatttg ggataaccag ttcatgactt tgctggagca gggacgcata caggaactgg atgccgtcag
0771 taacgaagag ctttccgcca ttgccggaaa gtcgacacat gaaatcaaaa cctgggtcgc cgcttttgcc
0841 gctatttctg cgtttggcaa ctggcgtagc gaagggcgtt attaccgccc aatcccggag tggattgccg
0911 gatttggctc gttaagcgcc agaacagaga actgaATATG CAGGAGAAGA TGATGAGTTA TCAGCCACAA
0981 ACCGAAGCCG CCACCAGCCG TTTTCTGAAT GTAGAAGAAG CGGGTAAAAC GCTGCGCATC CATTT
|
