MG1655
W3110
Search for
Japanese | English
Gene Report : lacI
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 9-1 (LD)  ,  OCR36,37-6 (LD 9C::Ap) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lacI
Related strains
     Strains Search Search strains by gene name:  lacI   Search strains by all related name:  lacI b0345 ECK0342 JW0336

General information  (Go to Linear View:)
 Gene Name lacI  
 Alternative name b0345,ECK0342,JW0336  
 Location, Length 365,652 - 366,734 (  -  ) ;   7.88 min ; 1083 (bp) ,   360 (aa) Go to Linear View
 Product DNA-binding transcriptional repressor  
 Operon Name mhpR-lacI  
 Note transcriptional repressor of the lac operon; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016564 - transcription repressor activity; GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0006350 - transcription  
 Function regulator; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0003677; DNA binding ( lacI )
GO:0003700; transcription factor activity ( lacI )
GO:0005515; protein binding ( lacI )
GO:0005622; intracellular ( lacI )
GO:0006350; transcription ( lacI )
GO:0006355; regulation of transcription, DNA-dependent ( lacI )
 PID 1786540  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene lacI ( EG10525 )  
    Number of removed 0 aa cleaved  
SWISS-PROT  ( Show details [ 1 more] )
  botton Entry name(Acc.no) LACI_ECOLI ( P03023 ; O09196; P71309; Q47338 )
    -  Protein name Lactose operon repressor.  
    -  Synonyms  
    -  Gene name Name=lacI; OrderedLocusNames=b0345;  

 Linear View (Whole Mode)
View Location
   355.0  –  380.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP lacI (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Lac Repressor  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Lactose operon repressor  
         PSI-BLAST HTH-type transcriptional repressor purR
    nr     (database updated : 2007.02.20 )
         BLAST lac repressor [Cloning vector pCMVLacI]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE CAMP_PHOSPHO_SITE    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    CRYSTALLIN_BETAGAMMA    HTH_LACI_FAMILY     

Other Cross-Reference
    COG COG1609K 
    EcoCyc lacI 

MMBR References
Nature. 1978;274(5673):765-9.
Sequence of the lacI gene.
Farabaugh PJ. ( 355891 )
J Mol Biol. 1988;200(2):239-51.
Missense mutation in the lacI gene of Escherichia coli. Inferences on the structure of the repressor protein.
Gordon AJ, Burns PA, Fix DF, Yatagai F, Allen FL, Horsfall MJ, Halliday JA, Gray J, Bernelot-Moens C, Glickman BW. ( 3286877 )
J Biol Chem. 1982;257:11740-11746
A termination site for Lac 1 transcription is between the CAP site and the lac promoter.
Horowitz, H., and T. Platt.
Biochim Biophys Acta. 1964;90:609-10.
A new method for the selection of mutants of escherichia coli forming beta-glactosidase cnstitutively.
Kesslar DP, Ricknberg HV. ( 14237870 )
Mol Gen Genet. 1969;105(1):74-83.
Mutations conferring quantitative and qualitative increases in beta-galactosidase activity in Escherichia coli.
Langridge J. ( 4899399 )
Mol Gen Genet. 1978;164(2):163-9.
Analysis of the regulatory mechanisms controlling the synthesis of the hexitol transport systems in Escherichia coli K12.
Lengeler J, Steinberger H. ( 360044 )
J Bacteriol. 1957;73(4):544-52.
Genetic control of raffinose utilization in Escherichia coli.
Lester G, Bonner DM. ( 13428689 )
Biochem Biophys Res Commun. 1965;20(2):230-4.
Genetic control of catabolite repression of the lac operon in Escherichia coli.
Loomis WF Jr, Magasanik B. ( 5321900 )
J Mol Biol. 1967;23(3):487-94.
The catabolite repression gene of the lac operon in Escherichia coli.
Loomis WF Jr, Magasanik B. ( 5340246 )
J Mol Biol. 1994;240(5):421-33.
Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence.
Markiewicz P, Kleina LG, Cruz C, Ehret S, Miller JH. ( 8046748 )
Proc Natl Acad Sci U S A. 1968;59(4):1259-64.
Mutants that mke more lac repressor.
Muller-Hill B, Crapo L, Gilbert W. ( 4870861 )
J Bacteriol. 1977;130(1):100-6.
Pseudoreversion of lactose operator-constitutive mutants.
Norwood WI, Sadler JR. ( 323217 )
Gene. 1986;42(3):283-92.
Cloning and characterization of several dominant-negative and tight-binding mutants of lac repressor.
Betz JL. ( 3732806 )
Eur J Biochem. 1975;59:491-509
Amino-acid sequence of lac repressor from Escherichia coli. Isolation, sequence analysis and sequence assembly of tryptic peptides and cyanogen-bromide fragments.
Beyreuther, K., K. Adler, E. Fanning, C. Murray, A. Klemm, and N. Geisler.
J Bacteriol. 1978;133(3):1358-67.
Permease-specific mutations in Salmonella typhimurium and Escherichia coli that release the glycerol, maltose, melibiose, and lactose transport systems from regulation by the phosphoenolpyruvate:sugar phosphotransferase system.
Saier MH Jr, Straud H, Massman LS, Judice JJ, Newman MJ, Feucht BU. ( 346569 )
J Mol Biol. 1971;59(2):273-305.
The nature of lactose operator constitive mutations.
Smith TF, Sadler JR. ( 4935786 )
Mol Gen Genet. 1980;178(1):225-7.
DNA sequence alteration resulting from a mutation impairing promoter function in the lac repressor gene.
Calos MP, Miller JH. ( 6770231 )

Sequences
Amino acid
FASTA format
0001 MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS 
0071 LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC 
0141 TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI 
0211 QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS 
0281 CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA 
0351 RQVSRLESGQ 

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             cgaagcggca tgcatttacg ttgacaccat 
-070 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat 
0001 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 
0071 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 
0141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 
0211 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 
0281 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 
0351 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc 
0421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 
0491 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 
0561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 
0631 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 
0701 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 
0771 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca 
0841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 
0911 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 
0981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
1051 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 
1121 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taa