MG1655
W3110
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Gene Report : lacZ
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 9-1 (LD)  ,  DE(argF-lac)169  ,  OCR36,37-6 (LD 9C::Ap) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lacZ
Related strains
     Strains Search Search strains by gene name:  lacZ   Search strains by all related name:  lacZ b0344 ECK0341 JW0335

General information  (Go to Linear View:)
 Gene Name lacZ  
 Alternative name b0344,ECK0341,JW0335  
 Location, Length 362,455 - 365,529 (  -  ) ;   7.81 min ; 3075 (bp) ,   1024 (aa) Go to Linear View
 Product beta-D-galactosidase  
 Operon Name lacZYA  
 Note GO_component: GO:0005737 - cytoplasm; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function enzyme; Degradation of small molecules: Carbon compounds  
 Gene Ontology GO:0003824; catalytic activity ( lacZ )
GO:0004553; hydrolase activity, hydrolyzing O-glycosyl compounds ( lacZ )
GO:0004565; beta-galactosidase activity ( lacZ )
GO:0005515; protein binding ( lacZ )
GO:0005975; carbohydrate metabolic process ( lacZ )
GO:0008152; metabolic process ( lacZ )
GO:0009341; beta-galactosidase complex ( lacZ )
GO:0016787; hydrolase activity ( lacZ )
GO:0016798; hydrolase activity, acting on glycosyl bonds ( lacZ )
GO:0030246; carbohydrate binding ( lacZ )
GO:0043169; cation binding ( lacZ )
 PID 1786539  
 EC number
  (KEGG Pathway)
 
3.2.1.23  
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene lacZ ( EG10527 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) BGAL_ECOLI ( P00722 )
    -  Protein name Beta-galactosidase  
    -  Synonyms EC 3.2.1.23, Lactase  
    -  Gene name Name=lacZ; OrderedLocusNames=b0344;  

 Linear View (Whole Mode)
View Location
   350.0  –  375.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP lacZ (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain I, Beta-Galactosidase (Chains I-P)  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Beta-galactosidase  
         PSI-BLAST Beta-galactosidase
    nr     (database updated : 2007.02.20 )
         BLAST beta-D-galactosidase [Escherichia coli K12]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    RGD    ATP_GTP_A    GLYCOSYL_HYDROL_F2_2    GLYCOSYL_HYDROL_F2_1     

Other Cross-Reference
    COG COG3250G 
    EcoCyc lacZ 

MMBR References
Gene. 1983;22(2-3):281-7.
A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome.
Hadley RG, Hu M, Timmons M, Yun K, Deonier RC. ( 6307829 )
Genetics 1963;48:157-169
The genetic basis of hypersynthesis of beta-galactoside.
Horiuchi, T., S. Horiuchi, and A. Novick.
J. Microbiol. Serol. 1970;16:83-89
Use of autocytotoxic beta-D-galactosides for selective growth of Salmonella typhimurium in the presence of coliforms.
Johnston, M. A., and H. Pivnick.
EMBO J. 1983;2(4):593-7.
Sequence of the lacZ gene of Escherichia coli.
Kalnins A, Otto K, Ruther U, Muller-Hill B. ( 6313347 )
Mol Gen Genet. 1969;105(1):74-83.
Mutations conferring quantitative and qualitative increases in beta-galactosidase activity in Escherichia coli.
Langridge J. ( 4899399 )
J Bacteriol. 1973;114(2):882-4.
Selective autocytotoxicity in a model system of Escherichia coli K-12 recombinants.
Martinez D, Whitehouse F Jr. ( 4574705 )
J Bacteriol. 1992;174:6256-6263
DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli.
Shi, W., Y. Zhou, J. Wild, J. Adler, and C. A. Gross.
J Bacteriol. 1994;176:1683-1688
An efficient Shine-Dalgarno sequence but not translation is necessary for lacZ mRNA stability in Escherichia coli.
Wagner, L. A., R. F. Gesteland, T. J. Dayhuff, and R. B. Weiss.

Sequences
Amino acid
FASTA format
0001 MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPPFASWRNS EEARTDRPSQ QLRSLNGEWR FAWFPAPEAV 
0071 PESWLECDLP EADTVVVPSN WQMHGYDAPI YTNVTYPITV NPPFVPTENP TGCYSLTFNV DESWLQEGQT 
0141 RIIFDGVNSA FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA GENRLAVMVL RWSDGSYLED QDMWRMSGIF 
0211 RDVSLLHKPT TQISDFHVAT RFNDDFSRAV LEAEVQMCGE LRDYLRVTVS LWQGETQVAS GTAPFGGEII 
0281 DERGGYADRV TLRLNVENPK LWSAEIPNLY RAVVELHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPL 
0351 LIRGVNRHEH HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG 
0421 MVPMNRLTDD PRWLPAMSER VTRMVQRDRN HPSVIIWSLG NESGHGANHD ALYRWIKSVD PSRPVQYEGG 
0491 GADTTATDII CPMYARVDED QPFPAVPKWS IKKWLSLPGE TRPLILCEYA HAMGNSLGGF AKYWQAFRQY 
0561 PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR QFCMNGLVFA DRTPHPALTE AKHQQQFFQF 
0631 RLSGQTIEVT SEYLFRHSDN ELLHWMVALD GKPLASGEVP LDVAPQGKQL IELPELPQPE SAGQLWLTVR 
0701 VVQPNATAWS EAGHISAWQQ WRLAENLSVT LPAASHAIPH LTTSEMDFCI ELGNKRWQFN RQSGFLSQMW 
0771 IGDKKQLLTP LRDQFTRAPL DNDIGVSEAT RIDPNAWVER WKAAGHYQAE AALLQCTADT LADAVLITTA 
0841 HAWQHQGKTL FISRKTYRID GSGQMAITVD VEVASDTPHP ARIGLNCQLA QVAERVNWLG LGPQENYPDR 
0911 LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH QWRGDFQFNI SRYSQQQLME TSHRHLLHAE 
0981 EGTWLNIDGF HMGIGGDDSW SPSVSAEFQL SAGRYHYQLV WCQK

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             tagctcactc attaggcacc ccaggcttta 
-070 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 
0001 atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 
0071 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 
0141 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg 
0211 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc 
0281 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac 
0351 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg 
0421 cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc 
0491 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt 
0561 gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc 
0631 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg 
0701 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt 
0771 aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc 
0841 gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg 
0911 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga 
0981 agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg 
1051 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg 
1121 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc 
1191 gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc 
1261 atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa 
1331 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc 
1401 taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc 
1471 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc 
1541 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg 
1611 cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat 
1681 ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca 
1751 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct 
1821 ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc 
1891 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc 
1961 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg 
2031 taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc 
2101 gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg 
2171 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga 
2241 tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg 
2311 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca 
2381 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta 
2451 ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct 
2521 cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc 
2591 aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg 
2661 ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc 
2731 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga 
2801 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca 
2871 gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa 
2941 gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag 
3011 tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata 
3081 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa aaacacaaac 
3151 ttttggatgt tcggtttatt ctttt