MG1655
W3110
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Gene Report : lacY
PEC Original Annotations
Essentiality
     Class non-essential
     References (PMID)  
     Deletion 9-1 (LD)  ,  DE(gpt-lac)5  ,  DE(argF-lac)169  ,  OCR36,37-6 (LD 9C::Ap) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  lacY
Related strains
     Strains Search Search strains by gene name:  lacY   Search strains by all related name:  lacY b0343 ECK0340 JW0334

General information  (Go to Linear View:)
 Gene Name lacY  
 Alternative name b0343,ECK0340,JW0334  
 Location, Length 361,150 - 362,403 (  -  ) ;   7.78 min ; 1254 (bp) ,   417 (aa) Go to Linear View
 Product lactose permease  
 Operon Name lacZYA  
 Note galactoside permease (lactose permease, M protein) (MFS family); GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process  
 Function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols,1.1.1 metabolism; carbon utilization; carbon compounds,4.2.A.1 transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Major Facili  
 Gene Ontology GO:0005215; transporter activity ( lacY )
GO:0005351; sugar:hydrogen ion symporter activity ( lacY )
GO:0005403; hydrogen:sugar symporter activity ( lacY )
GO:0005886; plasma membrane ( lacY )
GO:0006810; transport ( lacY )
GO:0008643; carbohydrate transport ( lacY )
GO:0015293; symporter activity ( lacY )
GO:0016020; membrane ( lacY )
GO:0016021; integral to membrane ( lacY )
 PID 1786538  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details [ 2 more] )
  botton Entry name(Acc.no) LACY_ECOLI ( P02920 )
    -  Protein name Lactose permease  
    -  Synonyms Lactose-proton symport  
    -  Gene name Name=lacY; OrderedLocusNames=b0343;  

 Linear View (Whole Mode)
View Location
   350.0  –  375.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP lacY (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of Lactose Permease  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Lactose permease  
         PSI-BLAST High-affinity glucose transporter SNF3
    nr     (database updated : 2007.02.20 )
         BLAST Lactose permease [Escherichia coli CFT073]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    GLYCOSAMINOGLYCAN    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    PROKAR_LIPOPROTEIN    LACY_1    LACY_2     

Other Cross-Reference
    EcoCyc lacY 

MMBR References
Nature. 1980;283(5747):537-40.
In vitro and in vivo products of E. coli lactose permease gene are identical.
Ehring R, Beyreuther K, Wright JK, Overath P. ( 6986561 )
Biochemistry. 1996;35(39):12909-14.
Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of EScherichia coli.
He MM, Sun J, Kaback HR. ( 8841135 )
Genetics 1963;48:157-169
The genetic basis of hypersynthesis of beta-galactoside.
Horiuchi, T., S. Horiuchi, and A. Novick.
J. Microbiol. Serol. 1970;16:83-89
Use of autocytotoxic beta-D-galactosides for selective growth of Salmonella typhimurium in the presence of coliforms.
Johnston, M. A., and H. Pivnick.
Proc Natl Acad Sci U S A. 1968;59(4):1259-64.
Mutants that mke more lac repressor.
Muller-Hill B, Crapo L, Gilbert W. ( 4870861 )
J Bacteriol. 1993;175:6269-6275
Lactose permease mutants which transport malto-oligosaccharides.
Olsen, S. G., K. M. Greene, and R. J. Brooker.
Proc Natl Acad Sci U S A. 1992;89:1524-1528
Functional complementation of internal deletion mutants in the lactose permease of Escherichia coli.
Bibi, E., and H. R. Kaback.
Prog Nucleic Acid Res Mol Biol. 1996;55:1-86.
Experimental analysis of global gene regulation in Escherichia coli.
Blumenthal RM, Borst DW, Matthews RG. ( 8787606 )
J Mol Biol. 1971;59(2):273-305.
The nature of lactose operator constitive mutations.
Smith TF, Sadler JR. ( 4935786 )
J Bacteriol. 1980;143(3):1374-83.
Pleiotropic mutations rendering Escherichia coli K-12 resistant to bacteriophage TP1.
Wandersman C, Moreno F, Schwartz M. ( 6997269 )
Nature. 1980;283(5747):541-5.
Sequence of the lactose permease gene.
Buchel DE, Gronenborn B, Muller-Hill B. ( 6444453 )

Sequences
Amino acid
FASTA format
0001 MYYLKNTNFW MFGLFFFFYF FIMGAYFPFF PIWLHDINHI SKSDTGIIFA AISLFSLLFQ PLFGLLSDKL 
0071 GLRKYLLWII TGMLVMFAPF FIFIFGPLLQ YNILVGSIVG GIYLGFCFNA GAPAVEAFIE KVSRRSNFEF 
0141 GRARMFGCVG WALCASIVGI MFTINNQFVF WLGSGCALIL AVLLFFAKTD APSSATVANA VGANHSAFSL 
0211 KLALELFRQP KLWFLSLYVI GVSCTYDVFD QQFANFFTSF FATGEQGTRV FGYVTTMGEL LNASIMFFAP 
0281 LIINRIGGKN ALLLAGTIMS VRIIGSSFAT SALEVVILKT LHMFEVPFLL VGCFKYITSQ FEVRFSATIY 
0351 LVCFCFFKQL AMIFMSVLAG NMYESIGFQG AYLVLGLVAL GFTLISVFTL SGPGPLSLLR RQVNEVA

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             gctgagcgcc ggtcgctacc attaccagtt 
-070 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag gaaatccatt 
0001 atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt tttatcatgg 
0071 gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat 
0141 tatttttgcc gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc 
0211 gggctgcgca aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgccgttc tttattttta 
0281 tcttcgggcc actgttacaa tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg 
0351 ttttaacgcc ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt 
0421 ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc atgttcacca 
0491 tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc gccgttttac tctttttcgc 
0561 caaaacggat gcgccctctt ctgccacggt tgccaatgcg gtaggtgcca accattcggc atttagcctt 
0631 aagctggcac tggaactgtt cagacagcca aaactgtggt ttttgtcact gtatgttatt ggcgtttcct 
0701 gcacctacga tgtttttgac caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg 
0771 tacgcgggta tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt ctttgcgcca 
0841 ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac tattatgtct gtacgtatta 
0911 ttggctcatc gttcgccacc tcagcgctgg aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc 
0981 gttcctgctg gtgggctgct ttaaatatat taccagccag tttgaagtgc gtttttcagc gacgatttat 
1051 ctggtctgtt tctgcttctt taagcaactg gcgatgattt ttatgtctgt actggcgggc aatatgtatg 
1121 aaagcatcgg tttccagggc gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt 
1191 gttcacgctt agcggccccg gcccgctttc cctgctgcgt cgtcaggtga atgaagtcgc ttaagcaatc 
1261 aatgtcggat gcggcgcgag cgccttatcc gaccaacata tcataacgga gtgatcgcat tgaacatgcc 
1331 aatgaccgaa agaataagag cagg