MG1655
W3110
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Gene Report : rpoH
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCL17,18-1 (D)  ,  OCL18 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rpoH
Related strains
     Strains Search Search strains by gene name:  rpoH   Search strains by all related name:  rpoH b3461 ECK3445 fam hin htpR JW3426

General information  (Go to Linear View:)
 Gene Name rpoH  
 Alternative name b3461,ECK3445,fam,hin,htpR,JW3426  
 Location, Length 3,597,952 - 3,598,806 (  -  ) ;   77.55 min ; 855 (bp) ,   284 (aa) Go to Linear View
 Product RNA polymerase, sigma 32 (sigma H) factor  
 Operon Name rpoH  
 Note RNA polymerase, sigma(32) factor; regulation of proteins induced at high temperatures; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0006350 - transcription; GO_process: GO:0009266 - response to temperature stimulus  
 Function factor; Global regulatory functions  
 Gene Ontology GO:0003677; DNA binding ( rpoH )
GO:0003700; transcription factor activity ( rpoH )
GO:0006350; transcription ( rpoH )
GO:0006352; transcription initiation ( rpoH )
GO:0006355; regulation of transcription, DNA-dependent ( rpoH )
GO:0006950; response to stress ( rpoH )
GO:0008270; zinc ion binding ( rpoH )
GO:0009408; response to heat ( rpoH )
GO:0016987; sigma factor activity ( rpoH )
 PID 1789871  
 EC number
  (KEGG Pathway)
 
 
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) RP32_ECOLI ( P00580 )
    -  Protein name RNA polymerase sigma-32 factor  
    -  Synonyms Heat shock regulatory protein F33.4  
    -  Gene name OrderedLocusNames=b3461, c4254, z4835, ECs4310;  

 Linear View (Whole Mode)
View Location
   3585.0  –  3610.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP rpoH (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain F, Crystal Structure Of The Rna Polymerase Holoenzyme From Thermus Thermophilus At 2.6a Resolution  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST RNA polymerase sigma-32 factor (Heat shock regulatory protein F33.4)  
         PSI-BLAST RNA polymerase sigma-32 factor
    nr     (database updated : 2007.02.20 )
         BLAST RNA polymerase sigma factor [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    SIGMA70_1    SIGMA70_2     

Other Cross-Reference
    COG COG0568K 
    EcoCyc rpoH 

MMBR References
Cell. 1984;38(2):383-90.
The htpR gene product of E. coli is a sigma factor for heat-shock promoters.
Grossman AD, Erickson JW, Gross CA. ( 6380765 )
J Bacteriol. 1985;161(3):939-43.
Mutations in the rpoH (htpR) gene of Escherichia coli K-12 phenotypically suppress a temperature-sensitive mutant defective in the sigma 70 subunit of RNA polymerase.
Grossman AD, Zhou YN, Gross C, Heilig J, Christie GE, Calendar R. ( 3882672 )
J Bacteriol. 1994;176:5648-5653
Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma32 in Escherichia coli.
Kanemori, M., H. Mori, and T. Yura.
Cell. 1984;38(1):175-82.
Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor.
Landick R, Vaughn V, Lau ET, VanBogelen RA, Erickson JW, Neidhardt FC. ( 6088062 )
J Bacteriol. 1983;153(2):597-603.
Molecular cloning and expression of a gene that controls the high-temperature regulon of Escherichia coli.
Neidhardt FC, VanBogelen RA, Lau ET. ( 6337122 )
Mol Gen Genet. 1984;195(1-2):10-6.
Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli.
Tobe T, Ito K, Yura T. ( 6092838 )
Proc Natl Acad Sci U S A. 1986;83:6959-6963
Heat shock response in Escherichia coli influences cell division.
Tsuchido, T., R. A. VanBogelen, and F. C. Neidhardt.
Proc Natl Acad Sci U S A. 1984;81:6803-6807
Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature.
Yura, T., T. Tobe, K. Ito, and T. Osawa.
J Bacteriol. 1988;170(8):3479-84.
Deletion and insertion mutations in the rpoH gene of Escherichia coli that produce functional sigma 32.
Calendar R, Erickson JW, Halling C, Nolte A. ( 2841288 )

Sequences
Amino acid
FASTA format
0001 MTDKMQSLAL APVGNLDSYI RAANAWPMLS ADEERALAEK LHYHGDLEAA KTLILSHLRF VVHIARNYAG 
0071 YGLPQADLIQ EGNIGLMKAV RRFNPEVGVR LVSFAVHWIK AEIHEYVLRN WRIVKVATTK AQRKLFFNLR 
0141 KTKQRLGWFN QDEVEMVARE LGVTSKDVRE MESRMAAQDM TFDLSSDDDS DSQPMAPVLY LQDKSSNFAD 
0211 GIEDDNWEEQ AANRLTDAMQ GLDERSQDII RARWLDEDNK STLQELADRY GVSAERVRQL EKNAMKKLRA 
0281 AIEA

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             ggtctgataa aacagtgaat gataacctcg 
-070 ttgctcttaa gctctggcac agttgttgct accactgaag cgccagaaga tatcgattga gaggatttga 
0001 atgactgaca aaatgcaaag tttagcttta gccccagttg gcaacctgga ttcctacatc cgggcagcta 
0071 acgcgtggcc gatgttgtcg gctgacgagg agcgggcgct ggctgaaaag ctgcattacc atggcgatct 
0141 ggaagcagct aaaacgctga tcctgtctca cctgcggttt gttgttcata ttgctcgtaa ttatgcgggc 
0211 tatggcctgc cacaggcgga tttgattcag gaaggtaaca tcggcctgat gaaagcagtg cgccgtttca 
0281 acccggaagt gggtgtgcgc ctggtctcct tcgccgttca ctggatcaaa gcagagatcc acgaatacgt 
0351 tctgcgtaac tggcgtatcg tcaaagttgc gaccaccaaa gcgcagcgca aactgttctt caacctgcgt 
0421 aaaaccaagc agcgtctggg ctggtttaac caggatgaag tcgaaatggt ggcccgtgaa ctgggcgtaa 
0491 ccagcaaaga cgtacgtgag atggaatcac gtatggcggc acaggacatg acctttgacc tgtcttccga 
0561 cgacgattcc gacagccagc cgatggctcc ggtgctctat ctgcaggata aatcatctaa ctttgccgac 
0631 ggcattgaag atgataactg ggaagagcag gcggcaaacc gtctgaccga cgcgatgcag ggtctggacg 
0701 aacgcagcca ggacatcatc cgtgcgcgct ggctggacga agacaacaag tccacgttgc aggaactggc 
0771 tgaccgttac ggcgtttccg ctgagcgtgt acgccagctg gaaaagaacg cgatgaaaaa attgcgtgct 
0841 gccattgaag cgtaatttcc gctattaagc agagaaccct ggatgagagt ccggggtttt tgttttttgg 
0911 gcctctgtaa taatcaattt cccctccggc aaaacgccaa tcccc